A5055089571- Hemoglobin structure and function
- Physiological and biochemical adaptations
- Marine Biology and Environmental Chemistry
- Developmental Biology and Gene Regulation
- Ocean Acidification Effects and Responses
- Echinoderm biology and ecology
- Environmental Toxicology and Ecotoxicology
- Bone Metabolism and Diseases
- Protein Structure and Dynamics
- Invertebrate Immune Response Mechanisms
- Photosynthetic Processes and Mechanisms
- Marine and coastal plant biology
- Polar Research and Ecology
- Aquaculture disease management and microbiota
- Alzheimer's disease research and treatments
- Nanoparticles: synthesis and applications
- Hemoglobinopathies and Related Disorders
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- Heme Oxygenase-1 and Carbon Monoxide
- Porphyrin Metabolism and Disorders
- Erythrocyte Function and Pathophysiology
- Calcium Carbonate Crystallization and Inhibition
- Animal Genetics and Reproduction
- NF-κB Signaling Pathways
National Research Council
2012-2024
Institute for Biomedical Research and Innovation
2019-2024
University of Perugia
2024
Ospedale Sant'Anna
2024
Azienda Socio Sanitaria Territoriale Lariana
2024
University of Naples Federico II
2023
Institute of Biosciences and Bioresources
2015-2020
Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy
2006-2019
Scuola Internazionale Superiore di Studi Avanzati
2015-2019
Institute of Protein Biochemistry
2009-2013
Sea urchins are emblematic models in developmental biology and display several characteristics that set them apart from other deuterostomes. To uncover the genomic cues may underlie these specificities, we generated a chromosome-scale genome assembly for sea urchin Paracentrotus lividus an extensive gene expression epigenetic profiles of its embryonic development. We found that, unlike vertebrates, retained ancestral chromosomal linkages but underwent very fast intrachromosomal order mixing....
Biodeterioration is a complex network of interactions between macro/micro-biological systems and organic/inorganic substrates involving physical chemical alterations, strictly related to their metabolic activities. Concerning microbial deterioration, finding correct approach counteract this process often difficult, requiring an understanding the kind alterations use methods that respect artwork human environmental health. Specific conservative remedial are used for aim. They comprise...
Abstract Titanium dioxide nanoparticles (TiO 2 NPs) are one of the most widespread-engineered particles in use for drug delivery, cosmetics and electronics. However, TiO NP safety is still an open issue, even ethical reasons. In this work, we investigated sea urchin Paracentrotus lividus immune cell model as a proxy to humans , elucidate potential pathway that can be involved persistent NP-immune interaction vivo . Morphology, phagocytic ability, changes activation/inactivation few...
Despite recent efforts in discovering novel long non-coding RNAs (lncRNAs) and unveiling their functions a wide range of biological processes applications as biotechnological or therapeutic tools are still at infancy. We have recently shown that AS Uchl1, natural lncRNA antisense to the Parkinson's disease-associated gene Ubiquitin carboxyl-terminal esterase L1 (Uchl1), is able increase UchL1 protein synthesis post-transcriptional level. Its activity requires two RNA elements: an embedded...
The development of multi-nucleated cells is critical for osteoclasts (OCs) maturation and function. Our objective was to extend knowledge on osteoclastogenesis, focusing pre-OC fusion timing behavior. RAW 264.7 cells, which a murine monocyte-macrophage cell line, provide valuable widely used tool in vitro studies osteoclastogenesis mechanisms. Cells were treated with the receptor activator nuclear factor κ-B ligand (RANKL) 1–4 days effects morphology, cytoskeletal organization, protein...
Titanium dioxide nanoparticles (TiO2NPs) are revolutionizing biomedicine due to their potential application as diagnostic and therapeutic agents. However, the TiO2NP immune-compatibility remains an open issue, even for ethical reasons. In this work, we investigated immunomodulatory effects of TiO2NPs in emergent proxy human non-mammalian model vitro basic translational immunology: sea urchin Paracentrotus lividus. To highlight on new insights into evolutionarily conserved intracellular...
The proteolysis of β‐amyloid (Aβ) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease neprilysin levels contributes to the accumulation amyloid deposits not only AD but also normal aging. analyzed mRNA and protein cerebral cortex from 10 cognitively elderly subjects with plaques (NA), cases AD, control free plaques. found significant both NA compared cases. Thereby, defect appears correlate Aβ deposition degeneration dementia.
Abstract Friedreich's ataxia (FRDA) is an untreatable disorder with neuro- and cardio-degenerative progression. This monogenic disease caused by the hyper-expansion of naturally occurring GAA repeats in first intron FXN gene, encoding for frataxin, a protein implicated biogenesis iron-sulfur clusters. As genetic defect interferes transcription, FRDA patients express normal frataxin but at insufficient levels. Thus, current therapeutic strategies are mostly aimed to restore physiological...
Objective: To report an ataxic variant of Alzheimer disease expressing a novel molecular phenotype.Design: Description phenotype associated with presenilin 1 mutation. Setting:The subject was outpatient who diagnosed at the local referral center.Patient: A 28-year-old man presented psychiatric symptoms and cerebellar signs, followed by cognitive dysfunction.Severe -amyloid (A) deposition accompanied neurofibrillary tangles cell loss in cerebral cortex Purkinje dendrite cerebellum.A gene...
Hemoglobin is the oxygen carrier in blood erythrocytes. Oxygen coordination mediated by α2β2 tetrameric structure via binding of ligand to heme iron atom. This essential for hemoglobin function blood. In last few years, expression has been found atypical sites, including brain. Transcripts α and β chains as well immunoreactivity have shown mesencephalic A9 dopaminergic neurons, whose selective degeneration leads Parkinson's disease. To gain further insights into roles brain, we examined its...
Ginger (Zingiber officinale) is commonly consumed as spice or herbal medicine with anti-inflammatory, antioxidant, and anticancer properties. It rich of many bioactive constituents, mainly gingerols shogaols. Although the constituents have been identified, molecular mechanisms ginger action are still limited, related signalling pathways not completely defined. Here, we used a simple ethanol/freeze-drying method to obtain new extract (GE), which was chemically characterised by...
Background: The erythrocyte sedimentation rate (ESR) is a routine and aspecific test that still widely used. reference-manual method for ESR determination the Westergren method. VES-MATIC 5 novel, fully automated, closed system based on modified This study conceived aim of comparing two analytical analysers, Test 1 5, with reference in practice. Methods: included 264 randomly analysed samples. A comparison between methods was performed, they were evaluated inter-run intra-run precision. In...
SUMMARY Specimens of Contracaecum spp. from Phalacrocorax auritus and Pelecanus occidentalis Florida were characterized by sequencing the small subunit mitochondrial ribosomal RNA gene ( rrnS ) PCR-based RFLP analysis same internal transcribed spacers (ITS) nuclear DNA. Analyses sequence data using MP UPGMA approaches yielded trees with similar topologies, delineating 3 main clusters. Ph. , morphologically assigned to C. rudolphii (s.l.), part cluster comprising also other 2 species complex...
Multicellular organisms have an immune system, which is essential for the survival of living beings. Interest in system has been expanded since common characteristics innate immunity between Drosophila melanogaster (Meigen, 1830) and mammals were discovered 1980. Since then, immunology mainly focused on adaptive that seems to be restricted vertebrates. Unlike immunity, one acquired after exposure a specific antigen (Ag) includes: antigen-presenting cells such as macrophages, proliferation B...
Hemoglobin (Hb) is the major protein in erythrocytes and carries oxygen (O2) throughout body. Recently, Hb has been found synthesized atypical sites, including brain. highly expressed A9 dopaminergic (DA) neurons of substantia nigra (SN), whose selective degeneration leads to Parkinson's disease (PD). Here we show that confers DA cells' susceptibility 1-methyl-4-phenylpyridinium (MPP+) rotenone, neurochemical cellular models PD. The toxic property does not depend on O2 binding associated...