A5030464140- Collagen: Extraction and Characterization
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Connective tissue disorders research
- Marine Biology and Environmental Chemistry
- Bone and Dental Protein Studies
- RNA Interference and Gene Delivery
- Proteoglycans and glycosaminoglycans research
- Cellular Mechanics and Interactions
- Marine Sponges and Natural Products
- Echinoderm biology and ecology
- Silk-based biomaterials and applications
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- Blood properties and coagulation
- Glycosylation and Glycoproteins Research
- Marine Ecology and Invasive Species
- Supramolecular Self-Assembly in Materials
- Developmental Biology and Gene Regulation
- TGF-β signaling in diseases
- Animal Genetics and Reproduction
- Enzyme Production and Characterization
- Osteoarthritis Treatment and Mechanisms
- Polysaccharides and Plant Cell Walls
- Biopolymer Synthesis and Applications
Université Claude Bernard Lyon 1
2012-2024
Centre National de la Recherche Scientifique
2008-2024
Biologie Tissulaire et Ingénierie Thérapeutique
2011-2024
Institut de Biologie et de Chimie des Protéines
1998-2016
Lyon College
1995-2010
Summry— Basement membrane structures, or their main component, type IV collagen, have been detected in all multicellular animal species, except sponges. We cancel this exception by the demonstration of collagenous sequences a new marine sponge species cDNA and genomic DNA studies. One these is long enough to demonstrate specific characteristics collagen chains. The 12 cysteines are at conserved positions carboxyl‐terminal non‐helical NC1 domain, as interruptions end triple helical domain....
Fibrillar collagens are the more abundant extracellular proteins. They form a metazoan-specific family, and highly conserved from sponge to human. Their structural physiological properties have been successfully used in food, cosmetic, pharmaceutical industries. On other hand, increase of jellyfish has led us consider this marine animal as natural product for food medicine. Here, we tested different Mediterranean species order investigate economic potential their collagens. We studied...
The use of tobacco plants as a novel expression system for the production human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding proα1(I) chain to generate transgenic expressing I. recombinant chains expressed disulfide‐bonded trimers and shown fold into stable triple helix. Moreover, procollagen was subsequently processed it occurs animals. Large amounts purified field grown plant material. data suggest that are valuable alternative various...
Sea urchins are emblematic models in developmental biology and display several characteristics that set them apart from other deuterostomes. To uncover the genomic cues may underlie these specificities, we generated a chromosome-scale genome assembly for sea urchin Paracentrotus lividus an extensive gene expression epigenetic profiles of its embryonic development. We found that, unlike vertebrates, retained ancestral chromosomal linkages but underwent very fast intrachromosomal order mixing....
We have characterized cDNA and genomic clones coding for a sponge collagen. The partial has an open reading frame encoding 547 amino acid residues. conceptual translation product contains probably incomplete triple-helical domain (307 acids) with one Gly-Xaa-Yaa-Zaa imperfection in the otherwise perfect Gly-Xaa-Yaa repeats carboxyl propeptide (240 that includes 7 cysteine Amino sequence comparisons indicate this collagen is homologous to vertebrate sea urchin fibrillar collagens. Partial...
Collagens are thought to represent one of the most important molecular innovations in metazoan line. Basement membrane type IV collagen is present all Eumetazoa and was found Homoscleromorpha, a sponge group with well-organized epithelium, which may first stage tissue differentiation during animal evolution. In contrast, spongin seems be demosponge-specific collagenous protein, can totally substitute an inorganic skeleton, such as well-known bath sponge. freshwater Ephydatia mülleri, we...
Transforming growth factor β (TGF-β) isoforms are secreted as inactive complexes formed through noncovalent interactions between the bioactive TGF-β entity and its N-terminal latency-associated peptide prodomain. Extracellular activation of latent complex is a crucial step in regulation function for tissue homeostasis. We show that fibrinogen-like (FBG) domain matrix glycoprotein tenascin-X (TNX) interacts physically with small vitro vivo, thus regulating bioavailability mature to cells by...
Tenascin‐X (TN‐X) is an extracellular matrix protein whose absence results in alteration of the mechanical properties connective tissue. To understand mechanisms integration TN‐X matrix, overlay blot assays were performed on skin extracts. A 100 kDa molecule interacting with was identified by this method and interaction abolished when extract digested chondroitinase. By solid‐phase assays, we showed that dermatan sulfate chains decorin bind to heparin‐binding site included within...
We isolated several overlapping cDNA clones covering the 4242 nucleotides of a Strongylocentrotus purpuratus transcript that codes for fibrillar procollagen chain. The sea urchin polypeptide includes 124-amino acid long amino pre-propeptide, 1064-amino alpha-chain inclusive 338 uninterrupted Gly-X-Y repeats, and 226-residue carboxyl-propeptide. distribution highly conserved cysteines within last domain together with structural configuration amino-propeptide organization corresponding coding...
mRNA-based vaccines have made a leap forward since the SARS-CoV-2 pandemic and are currently used to develop anti-infectious therapies. If selection of delivery system an optimized mRNA sequence two key factors reach in vivo efficacy, optimal administration route for those remains unclear. We investigated influence lipid components immunization regarding intensity quality humoral immune responses mice. The immunogenicity HIV-p55Gag encoded encapsulated into D-Lin-MC3-DMA or GenVoy-ionizable...
Two previously described sponge cDNAs, EmC4 and C23, respectively encoding a short chain collagen fibrillar collagen, were used to characterize gene families in freshwater sponge. detected several clones when screen cDNA library. overlapping clones, EmC13 1 2, sequenced appeared highly homologous EmC4. Contrarily hybridized with 10-12 fragments of genomic DNA digested restriction endonucleases 10 times more positive than C23 The clone G41 contained two closely related genes, COLNF13,...
Tenascin‐X is an extracellular matrix protein whose absence leads to Ehlers‐Danlos syndrome in humans, characterized mainly by disorganisation of collagen and elastic fibril networks. After producing recombinant full‐length tenascin‐X mammalian cells, we find that this assembled into disulfide‐linked oligomers. Trimers were the predominant form observed using rotary shadowing. By solid phase interaction studies, demonstrate interacts with types I, III V fibrillar molecules when they are...
Collagens are often considered a metazoan hallmark, with the fibril-forming fibrillar collagens present from sponges to human. From evolutionary studies, three collagen clades (named A, B, and C) have been defined shown be in mammals, whereas emergence of A B predates protostome/deuterostome split. Moreover, several C clade chains some invertebrate deuterostome genomes but not protostomes whose sequenced. The newly sequenced choanoflagellate Monosiga brevicollis, demosponge Amphimedon...
Cytotoxicity of nanoparticles and their sub-lethal effect on cell behavior fate are a high topic studies in the nanomaterial field. With an explosion nanoparticle types (size, shape, polarity, stiffness, composition, etc.), Drosophila has become attractive animal model for throughput analysis these nanocarriers drug delivery field with applications cancer therapy, or simply to generate fast complete cytotoxic study peculiar nanoparticle. In respect that, we have conducted cellulo poly(lactic...
Adhesive properties of tenascin‐X (TN‐X) were investigated using TN‐X purified from bovine skin and recombinant proteins encompassing the RGD sequence located within tenth fibronectin type‐III domain, fibrinogen‐like domain. Osteosarcoma (MG63) bladder carcinoma cells (ECV304) shown to adhere TN‐X, but did not spread assemble actin stress fibers. Both cell types adhered harboring contiguous domains 9 10 (FNX 9‐10) FNX domain alone. This adhesion 9‐10 was be mediated by αvβ3 integrin,...