A5068553618- Protease and Inhibitor Mechanisms
- Nitric Oxide and Endothelin Effects
- Cell Adhesion Molecules Research
- Connective tissue disorders research
- Peptidase Inhibition and Analysis
- Bone and Dental Protein Studies
- TGF-β signaling in diseases
- Connective Tissue Growth Factor Research
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
- Eicosanoids and Hypertension Pharmacology
- Collagen: Extraction and Characterization
- Biochemical and Structural Characterization
- Periodontal Regeneration and Treatments
- Wound Healing and Treatments
- Synthesis and Catalytic Reactions
- Angiogenesis and VEGF in Cancer
- Amino Acid Enzymes and Metabolism
- Protein Hydrolysis and Bioactive Peptides
- Skin and Cellular Biology Research
- Glycosylation and Glycoproteins Research
- Silk-based biomaterials and applications
- Receptor Mechanisms and Signaling
- Bone health and treatments
- Corneal Surgery and Treatments
Université Claude Bernard Lyon 1
2015-2024
Biologie Tissulaire et Ingénierie Thérapeutique
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Lyon College
2022
Inserm
2020
École Normale Supérieure de Lyon
2020
Centre National pour la Recherche Scientifique et Technique (CNRST)
2016
Institut de Biologie et de Chimie des Protéines
2001-2013
Universidad de Málaga
2012
Masimo (United States)
2012
A detailed comparison of the oxidation five compounds closely related to l-arginine (Arg) by purified recombinant neuronal and macrophage NO synthases (NOS I NOS II) was performed. Homo-l-arginine (homo-Arg) is oxidized both NOSs in presence NADPH with major formation homo-l-citrulline, a molar ratio close 1, minor Nω-hydroxyhomo-l-arginine (homo-NOHA). Oxidation homo-NOHA two also leads homocitrulline 1:1 ratio. On contrary, Nω-hydroxynor-l-arginine (nor-NOHA) very poor substrate II, which...
Type I fibrillar collagen is the most abundant protein in human body, crucial for formation and strength of bones, skin, tendon. Proteolytic enzymes are essential initiation assembly fibrils by cleaving off propeptides. We report that Mep1a −/− Mep1b mice revealed lower amounts mature compared with WT exhibited significantly reduced deposition along markedly decreased tissue tensile strength. While exploring mechanism this phenotype, we found cleavage full-length procollagen heterotrimers...
Abstract Fibrillar collagen molecules are synthesized as precursors, procollagens, with large propeptide extensions. While a homotrimeric form (three α1 chains) has been reported in embryonic tissues well diseases (cancer, fibrosis, genetic disorders), type I usually occurs heterotrimer (two chains and one α2 chain). Inside the cell, role of C-terminal propeptides is to gather together correct combination three α during molecular assembly, but how this for different forms same so far...
A disintegrin and metalloproteinase with thrombospondin type I motif (ADAMTS)2, 3, 14 are collectively named procollagen N-proteinases (pNPs) because of their specific ability to cleave the aminopropeptide fibrillar procollagens. Several reports also indicate that they could be involved in other biological processes, such as blood coagulation, development, male fertility, but potential substrates associated these activities remain unknown. Using recently described N-terminal amine isotopic...
ABSTRACT CENTA, a chromogenic cephalosporin, is readily hydrolyzed by β-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used detection β-lactamase-producing strains on agar plates, should quite useful kinetic studies and enzymes in crude extracts chromatographic fractions.
Tight regulation of collagen fibril deposition in the extracellular matrix is essential for normal tissue homeostasis and repair, defects which are associated with several degenerative or fibrotic disorders. A key regulatory step assembly C-terminal proteolytic processing soluble procollagen precursors. This step, carried out mainly by bone morphogenetic protein-1/tolloid-like proteinases, itself subject to C-proteinase enhancer proteins (PCPEs) can dramatically increase proteinase activity,...
The correct balance between collagen synthesis and degradation is essential for almost every aspect of life, from development to healthy aging, reproduction wound healing. When this compromised by external or internal stress signals, it very often leads disease as the case in fibrotic conditions. Fibrosis occurs context defective tissue repair characterized excessive, aberrant debilitating deposition fibril-forming collagens. Therefore, numerous proteins involved biosynthesis fibrillar...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTThe New α-Amino Acid Nω-Hydroxy-nor-l-arginine: a High-Affinity Inhibitor of Arginase Well Adapted To Bind to Its Manganese ClusterJ. Custot, C. Moali, M. Brollo, J. L. Boucher, Delaforge, D. Mansuy, P. Tenu, and ZimmermannView Author Information Laboratoire de Chimie et Biochimie Pharmacologiques Toxicologiques, URA 400 CNRS Université René Descartes, 45 rue des Saints-Pères 75270 Paris Cedex 06, France CNRS, 1116, Bât. 432 XI, 91405 Orsay,...
Bone morphogenetic protein-1 (BMP-1) and the tolloid-like metalloproteinases control several aspects of embryonic development tissue repair. Unlike other proteinases whose activities are regulated mainly by endogenous inhibitors, regulation BMP-1/tolloid-like relies mostly on proteins that stimulate activity. Among these, procollagen C-proteinase enhancers (PCPEs) markedly increase proteinase activity fibrillar procollagens, in a substrate-specific manner. Here, we performed detailed...
Procollagen C-proteinase enhancer-1 (PCPE-1) is a secreted protein that specifically accelerates proteolytic release of the C-propeptides from fibrillar procollagens, crucial step in fibril assembly. As such, it potential therapeutic target to improve tissue repair and prevent fibrosis, major cause mortality worldwide. Here we present crystal structure active CUB1CUB2 fragment PCPE-1 bound C-propeptide trimer procollagen III (CPIII). This shows two CUB domains bind different chains CPIII...
à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Transforming growth factor-β (TGF-β) isoforms are secreted as inactive complexes formed through non-covalent interactions between bioactive TGF-β entities and their N-terminal pro-domains called latency-associated peptides (LAP). Extracellular activation of latent within this complex is a crucial step in the regulation activity for tissue homeostasis immune cell function. We previously showed that matrix glycoprotein Tenascin-X (TN-X) interacted with small triggered cytokine into TGF-β. This...
BMP-1–mediated cleavage of TSP-1 reduces cell adhesion and promotes TGF-β signaling.