A5066906074- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Hearing, Cochlea, Tinnitus, Genetics
- Nanoparticle-Based Drug Delivery
- DNA Repair Mechanisms
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Hearing Loss and Rehabilitation
- Electron and X-Ray Spectroscopy Techniques
- Characterization and Applications of Magnetic Nanoparticles
- Cellular Mechanics and Interactions
- Marine animal studies overview
- Microtubule and mitosis dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bryophyte Studies and Records
- Epigenetics and DNA Methylation
- Plant Reproductive Biology
- Electrohydrodynamics and Fluid Dynamics
- Nanoparticles: synthesis and applications
- T-cell and B-cell Immunology
- Botany and Plant Ecology Studies
- Advanced Electron Microscopy Techniques and Applications
- Graphene and Nanomaterials Applications
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
Massachusetts Eye and Ear Infirmary
2020-2024
Harvard University
2020-2024
University of Massachusetts Chan Medical School
2022-2023
Moscow State University
2022
Lomonosov Moscow State University
2013-2022
International Medical Research (Germany)
2019
Research & Development Corporation
2019
Skolkovo Foundation
2019
Moscow Power Engineering Institute
2017
Moscow Aviation Institute
2017
Abstract Usher syndrome type 1 F (USH1F), caused by mutations in the protocadherin-15 gene ( PCDH15 ), is characterized congenital deafness, lack of balance, and progressive blindness. In hair cells, receptor cells inner ear, a component tip links, fine filaments which pull open mechanosensory transduction channels. A simple addition therapy for USH1F challenging because coding sequence too large adeno-associated virus (AAV) vectors. We use rational, structure-based design to engineer...
Superparamagnetic carbon-encapsulated iron carbide nanoparticles have been synthesized and tested for cytotoxicity.
The current progress in the study of spatial organization interphase chromosomes became possible owing to development chromosome conformation capture (3C) protocol. crucial step this protocol is proximity ligation—preferential ligation DNA fragments assumed be joined within nuclei by protein bridges and solubilized as a common complex after formaldehyde cross-linking cleavage. Here, we show that substantial, some cases major, part not from cross-linked treated with restriction...
The development of multi-nucleated cells is critical for osteoclasts (OCs) maturation and function. Our objective was to extend knowledge on osteoclastogenesis, focusing pre-OC fusion timing behavior. RAW 264.7 cells, which a murine monocyte-macrophage cell line, provide valuable widely used tool in vitro studies osteoclastogenesis mechanisms. Cells were treated with the receptor activator nuclear factor κ-B ligand (RANKL) 1–4 days effects morphology, cytoskeletal organization, protein...
Mammalian sperm show an unusual and heavily compacted genomic packaging state. In addition to its role in organizing the compact hydrodynamic head, it has been proposed that chromatin architecture helps program gene expression early embryo. Scores of genome-wide surveys have reported patterns accessibility, nucleosome localization, histone modification, chromosome folding. Here, we revisit these studies light recent reports obtained from mouse epididymis are contaminated with low levels...
The contribution of nucleoli to the cellular stress response has been discussed for over a decade. Stress-induced inhibition RNA polymerase I-dependent transcription is hypothesized as possible effector program in such response. In this study, we report new mechanism by which ribosomal DNA can be inhibited stress. Specifically, demonstrate that mild hypoosmotic induces stabilization R loops genes and thus provokes nucleoli-specific damage response, governed ATM- Rad3-related (ATR) kinase....
Mitochondria are important regulators of tumour growth and progression due to their specific role in cancer metabolism modulation apoptotic pathways. In this paper we describe that mitochondria-targeted antioxidant SkQ1 designed as a conjugate decyl-triphenylphosphonium cation (TPP+) with plastoquinone, suppressed the fibrosarcoma HT1080 rhabdomyosarcoma RD cells culture xenograft nude mouse model. Under same conditions, no detrimental effect on cell primary human subcutaneous fibroblasts...
Excess noise damages sensory hair cells, resulting in loss of synaptic connections with auditory nerves and, some cases, hair-cell death. The cellular mechanisms underlying mechanically induced damage and subsequent repair are not completely understood. Hair cells neuromasts larval zebrafish structurally functionally comparable to mammalian but undergo robust regeneration following ototoxic damage. We therefore developed a model for this highly tractable system. Free swimming larvae exposed...
A detailed understanding of the principles structural organization genetic material is great importance for elucidating mechanisms differential regulation genes in development. Modern ideas about spatial genome are based on a microscopic analysis chromatin structure and molecular data DNA-DNA contact using Chromatin conformation capture (3C) technology, ranging from "polymer melt" model to hierarchical folding concept. Heterogeneity depending its functional state cell cycle progression...
A new type of superparamagnetic nanoparticles with chemical formula Fe7C3@C (MNPs) showed higher value magnetization compared to traditionally used iron oxide-based as was shown in our previous studies. The vitro biocompatibility tests demonstrated that the MNPs display high efficiency cellular uptake and do not affect cyto-physiological parameters cultured cells. These effective magnetocontrollability homogeneous liquids but their behavior cytoplasm living cells under effect magnetic field...
Magnetite–gold dumbbell nanoparticles are essential for biomedical applications due to the presence of two surfaces with different chemical natures and potential combination magnetic plasmonic properties. Here, remote actuation Fe3O4-Au hybrid particles in a rotating (1 Hz, 7 mT), static (7 mT) or pulsed low-frequency (31 175 mT, 30 s pulse/30 pause) field was studied. The were synthesized by high-temperature wet chemistry protocol exhibited superparamagnetic properties saturation...
Theranostics application of superparamagnetic nanoparticles based on magnetite and maghemite is impeded by their toxicity. The use additional protective shells significantly reduced the magnetic properties nanoparticles. Therefore, iron carbides pure coated with multiple layers onion-like carbon sheath seem to be optimal for biomedicine. Fluorescent markers associated provide reliable means multimodal visualization. Here, biocompatibility graphite-like shell labeled Alexa 647 fluorescent...
During a cell's lifespan, DNA break formation is common event, associated with many processes, from replication to apoptosis. Most of breaks are readily repaired, but some meant persist in time, such as the chromosome ends, protected by telomeres. Besides them, eukaryotic genomes comprise shorter stretches interstitial telomeric repeats. We assumed that latter may also be time. In zebrafish and mouse embryos, cells containing numerous breakage foci were identified. These not apoptosis or...
The Ukrainian protests of November–December 2013 owed their success to the relocation modular repertoires contention (street protests, sit-ins and barricades) onto soil adaptation local traditions. Particular attention is given Veche (a gathering community members) Sich military camp Cossacks). communitarian model institutional transfers better suited for case than entrepreneurial model: were initiated, organized sustained by ordinary people as opposed leaders opposition parties. Three mass...
Abstract Excess noise damages sensory hair cells, resulting in loss of synaptic connections with auditory nerves and, some cases, hair-cell death. The cellular mechanisms underlying mechanically induced damage and subsequent repair are not completely understood. Hair cells neuromasts larval zebrafish structurally functionally comparable to mammalian but undergo robust regeneration following ototoxic damage. We therefore developed a model for this highly tractable system. Free swimming larvae...
The uptake of superparamagnetic Fe7C3@C nanoparticles into living cells and their behavior once inside the cell was investigated.The used were shown to absorb nanoparticle aggregates over first 30 minutes.After absorption, these moved towards center accumulated near nucleus.No toxic effects on physiology observed.In a magnetic field, particles aligned in along lines shifted magnet's side.During long-term cultivation, ultimately discarded via exocytosis.
ABSTRACT Mammalian sperm exhibit an unusual and heavily-compacted genomic packaging state. In addition to its role in organizing the compact hydrodynamic head, it has been proposed that chromatin architecture helps program gene expression early embryo. Scores of genome-wide surveys have reported patterns accessibility, histone localization, modification, chromosome folding. Here, we revisit these studies light recent reports obtained from mouse epididymis are contaminated with low levels...