A5054809339- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Nanoparticle-Based Drug Delivery
- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Characterization and Applications of Magnetic Nanoparticles
- Microtubule and mitosis dynamics
- Electron and X-Ray Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Hematopoietic Stem Cell Transplantation
- Nanoparticles: synthesis and applications
- Tissue Engineering and Regenerative Medicine
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Oral health in cancer treatment
- Telomeres, Telomerase, and Senescence
- Bone Metabolism and Diseases
- Elasticity and Material Modeling
- Skin and Cellular Biology Research
Lomonosov Moscow State University
2014-2022
Moscow State University
2022
International Medical Research (Germany)
2019
Research & Development Corporation
2019
Skolkovo Foundation
2019
Ministry of Health of the Russian Federation
2011-2013
Academy of Medical Sciences
2010
Russian Academy of Sciences
2010
The efficacy and the safety of administration multipotent mesenchymal stromal cells (MMSCs) for acute graft-versus-host disease (aGVHD) prophylaxis following allogeneic hematopoietic cell transplantation (HSCT) were studied. This prospective clinical trial was based on random patient allocation to two groups receiving (1) standard GVHD (2) combined with MMSCs infusion. Bone marrow from stem donors cultured administered recipients at doses 0.9–<svg...
The development of multi-nucleated cells is critical for osteoclasts (OCs) maturation and function. Our objective was to extend knowledge on osteoclastogenesis, focusing pre-OC fusion timing behavior. RAW 264.7 cells, which a murine monocyte-macrophage cell line, provide valuable widely used tool in vitro studies osteoclastogenesis mechanisms. Cells were treated with the receptor activator nuclear factor κ-B ligand (RANKL) 1–4 days effects morphology, cytoskeletal organization, protein...
A detailed understanding of the principles structural organization genetic material is great importance for elucidating mechanisms differential regulation genes in development. Modern ideas about spatial genome are based on a microscopic analysis chromatin structure and molecular data DNA-DNA contact using Chromatin conformation capture (3C) technology, ranging from "polymer melt" model to hierarchical folding concept. Heterogeneity depending its functional state cell cycle progression...
A new type of superparamagnetic nanoparticles with chemical formula Fe7C3@C (MNPs) showed higher value magnetization compared to traditionally used iron oxide-based as was shown in our previous studies. The vitro biocompatibility tests demonstrated that the MNPs display high efficiency cellular uptake and do not affect cyto-physiological parameters cultured cells. These effective magnetocontrollability homogeneous liquids but their behavior cytoplasm living cells under effect magnetic field...
Magnetite–gold dumbbell nanoparticles are essential for biomedical applications due to the presence of two surfaces with different chemical natures and potential combination magnetic plasmonic properties. Here, remote actuation Fe3O4-Au hybrid particles in a rotating (1 Hz, 7 mT), static (7 mT) or pulsed low-frequency (31 175 mT, 30 s pulse/30 pause) field was studied. The were synthesized by high-temperature wet chemistry protocol exhibited superparamagnetic properties saturation...
Theranostics application of superparamagnetic nanoparticles based on magnetite and maghemite is impeded by their toxicity. The use additional protective shells significantly reduced the magnetic properties nanoparticles. Therefore, iron carbides pure coated with multiple layers onion-like carbon sheath seem to be optimal for biomedicine. Fluorescent markers associated provide reliable means multimodal visualization. Here, biocompatibility graphite-like shell labeled Alexa 647 fluorescent...
During a cell's lifespan, DNA break formation is common event, associated with many processes, from replication to apoptosis. Most of breaks are readily repaired, but some meant persist in time, such as the chromosome ends, protected by telomeres. Besides them, eukaryotic genomes comprise shorter stretches interstitial telomeric repeats. We assumed that latter may also be time. In zebrafish and mouse embryos, cells containing numerous breakage foci were identified. These not apoptosis or...
The uptake of superparamagnetic Fe7C3@C nanoparticles into living cells and their behavior once inside the cell was investigated.The used were shown to absorb nanoparticle aggregates over first 30 minutes.After absorption, these moved towards center accumulated near nucleus.No toxic effects on physiology observed.In a magnetic field, particles aligned in along lines shifted magnet's side.During long-term cultivation, ultimately discarded via exocytosis.