A5032253265- RNA Research and Splicing
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- HIV Research and Treatment
- RNA regulation and disease
- Monoclonal and Polyclonal Antibodies Research
- Plant tissue culture and regeneration
- DNA Repair Mechanisms
- Viral-associated cancers and disorders
- DNA and Nucleic Acid Chemistry
- Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
- Transgenic Plants and Applications
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Clay minerals and soil interactions
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Therapeutic Uses of Natural Elements
- Prion Diseases and Protein Misfolding
- Iron oxide chemistry and applications
- Cancer-related gene regulation
- Seed Germination and Physiology
Lomonosov Moscow State University
2014-2024
Moscow State University
2008-2024
Russian Cancer Research Center NN Blokhin
2016
Russian Academy of Sciences
2010
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2008
V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry
2007
Engelhardt Institute of Molecular Biology
2007
Russian State Agrarian University - Moscow Timiryazev Agricultural Academy
2006
National Research Center for Hematology Russian Academy of Medical Sciences
2004
Abstract The goals of the study were: (1) to explore communication between human mesenchymal stem cells (MSC) and rat cardiac myocytes resulting in differentiation and, (2) evaluate role mitochondria it. Light fluorescence microscopy as well scanning electron revealed that after co‐cultivation, formed intercellular contacts transient exchange with cytosolic elements could be observed. transport entity had no specific direction. Noticeably, also transferred recipient a unidirectional fashion...
Human immunodeficiency virus (HIV) infection is associated with B-cell malignancies in patients though HIV-1 not able to infect B-cells. The rate of lymphomas HIV-infected individuals remains high even under the combined antiretroviral therapy (cART) that reconstitutes immune function. Thus, contribution oncogenesis enigmatic. induces oxidative stress and DNA damage infected cells via multiple mechanisms, including viral Tat protein. We have detected elevated levels reactive oxygen species...
The mechanical damage that often precedes the penetration of a leaf by pathogen promotes activation pectin methylesterase (PME); PME leads to emission methanol, resulting in "priming" effect on intact leaves, which is accompanied an increased sensitivity Tobacco mosaic virus (TMV) and resistance bacteria. In this study, we revealed mRNA levels methanol-inducible gene encoding Nicotiana benthamiana aldose 1-epimerase-like protein (NbAELP) leaves plants are very low compared with roots....
In this study, we report a method for the suppression of Escherichia coli (E. coli) vitality by means therapeutic ultrasound irradiation (USI) using biocompatible silicon nanoparticles as cavitation sensitizers. Silicon without (SiNPs) and with polysaccharide (dextran) coating (DSiNPs) were used. Both types nontoxic to Hep 2 cells up concentration mg/mL. The treatment bacteria application 1 W/cm2 USI resulted in reduction their viabilities 35 72% SiNPs DSiNPs, respectively. higher bacterial...
Abstract Despite the success of combination antiretroviral therapy, people living with human immunodeficiency virus (HIV) still have an increased risk Epstein−Barr (EBV)‐associated B cell malignancies. In HIV setting, physiology is altered by coexistence HIV‐infected cells and chronic action secreted viral proteins, for example, HIV‐1 Tat that, once released, efficiently penetrates noninfected cells. We modeled on ectopically expressing or TatC22G mutant in two lymphoblastoid lines. The...
Micellar peroxidase-catalyzed synthesis of chiral polyaniline (PANI) in the presence dodecylbenzenesulfonic acid (DBSA) was developed. The effect DBSA concentration on catalytic efficiency horseradish and palm tree peroxidases examined. Favorable conditions for enzymatic PANI, determined by a multiple factors design, demonstrated that PANIs with highest chirality were produced low concentrations optically active camphorsulphonic (CSA). Unexpectedly, PANI also synthesized absence CSA feed....
It has been demonstrated elsewhere that a high concentration of an antigen within the nucleolus may prevent its proper recognition by specific antibodies. In this study, authors found short proteinase treatment allowed for detection antigens in nucleoli. The described approach is compatible with simultaneous observation proteins fused to fluorescent tags and preembedding electron microscopy. appears method can be useful situations when antibodies disturbed density cellular structures or...
Nanocontainers based on solid materials have great potential for drug delivery applications. However, since nanocontainer-mediated can alter the internalization pathways and metabolism, it is important to find out what are mechanisms of cancer cell death induced by nanocontainers and, moreover, possible regulate them. Here, we report detailed investigation kinetics intracellular spatial distribution porous silicon nanoparticles (PSi NPs) loaded with doxorubicin (DOX) response cells treatment...
HIV-1 infects T cells, but the most frequent AIDS-related lymphomas are of B-cell origin. Molecular mechanisms HIV-1-induced oncogenic transformation B cells remain largely unknown. Tat protein may participate in this process by penetrating and regulating gene expression cells. Both immune cancer can reprogram communications between extracellular signals intracellular signaling pathways via Akt/mTORC1 pathway, which plays a key role cellular response to various stimuli including viral...
The mechanisms by which the supramolecular order is formed inside cell nucleus remain poorly understood. So far, two major hypotheses – ordered assembly and stochastic self-organization have been discussed. To determine mechanism responsible for formation of nuclear envelope, cells overexpressing one envelope proteins (lamin A, lamin B1, pom121 or ndc1) were investigated. According to model, presence an excessive amount a component has no effect in normal structure because it programmed...
A novel extraction protocol for cells cultured on coverslips is described. Observations of the process in a perfusion chamber reveal that all mitotic stages are not detached from during extraction, and can be recognized using phase contrast images. We studied extracted cell morphology distribution major scaffold component - topoisomerase IIalpha, metaphase anaphase cells. An 2M NaCl leads to destruction chromosomes at light microscope level. Immunogold studies demonstrate only residual...
Electron microscopic study of cardiomyocytes taken from healthy Wistar and OXYS rats naked mole (
During evolution, viruses had to adapt an increasingly complex environment of eukaryotic cells. Viral proteins that need enter the cell nucleus or associate with nucleoli possess nuclear localization signals (NLSs) and nucleolar (NoLSs) for accumulation, respectively. As viral are relatively small, acquisition novel sequences seems be a more complicated task than eukaryotes. Here, we carried out comprehensive analysis basic domain (BD) HIV-1 Tat show how might evolve NLSs NoLSs without...
Some nuclear envelope proteins are localised to both the and endoplasmic reticulum; therefore, it seems plausible that even small amounts of these can influence organisation reticulum. A simple method study possible effects on reticulum is analyze protein overexpression. Here, we demonstrate Lap2β overexpression induce formation cytoplasmic vesicular structures derived from membranes. Correlative light electron microscopy demonstrated were composed a series closely apposed membranes...
Microtubule (MT) inhibitors show anti-cancer activity in a wide range of tumors vitro and demonstrate high clinical efficacy. To date they are routinely included into many chemotherapeutic regimens. While the mechanisms MT inhibitors' interactions with tubulin have been well-established, relationship between their concentration effect on neoplastic cells is not completely understood. The common notion that tumor most vulnerable during division all block them mitosis induce mitotic...
Using an original high-salt extraction protocol, we observed a novel chromosome substructure, referred to as the peripheral scaffold. This domain contained perichromosomal layer proteins pKi-67, B23/nucleophosmin and fibrillarin, but no DNA fragments (i.e., loop bases were not associated with scaffold). Modern models of organization do predict existence scaffold domain, thus our observations have conceptual implications for understanding architecture.
Abstract Background The origin of the selective nuclear protein import machinery, which consists pore complexes and adaptor molecules interacting with localization signals (NLSs) cargo molecules, is one most important events in evolution eukaryotic cells. How proteins were selected for into forming nucleus remains an open question. Results Here, we demonstrate that functional NLSs may be integrated nucleotide-binding domains both prokaryotic coevolve these domains. Conclusion presence...