A5063639723- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Microbial Natural Products and Biosynthesis
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Cancer therapeutics and mechanisms
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Molecular Sensors and Ion Detection
- Free Radicals and Antioxidants
- Coenzyme Q10 studies and effects
- Antimicrobial Peptides and Activities
- Bioactive Compounds and Antitumor Agents
- Redox biology and oxidative stress
- Marine Sponges and Natural Products
- Metabolomics and Mass Spectrometry Studies
- Cancer-related gene regulation
- Chemical Synthesis and Analysis
- Neuroscience and Neuropharmacology Research
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Metabolism and Genetic Disorders
- Mass Spectrometry Techniques and Applications
Lomonosov Moscow State University
2016-2025
Moscow State University
2010-2019
Sechenov University
2013
Institute of Biomedical Chemistry
1992
In order to accelerate drug discovery, a simple, reliable, and cost-effective system for high-throughput identification of potential antibiotic mechanism action is required. To facilitate such screening new antibiotics, we created double-reporter not only antimicrobial activity detection but also simultaneous sorting antimicrobials into those that cause ribosome stalling induce the SOS response due DNA damage. this reporter system, red fluorescent protein gene rfp was placed under control...
The mitochondrial membrane potential (∆Ψ) is the driving force providing electrical component of total transmembrane hydrogen ions generated by proton pumps, which utilized ATP synthase. role ∆Ψ not limited to its in bioenergetics since it takes part other important intracellular processes, leads mandatory requirement homeostasis ∆Ψ. Conventionally, living cells estimated fluorescence probes such as rhodamine 123, tetramethylrodamine, etc. However, when assessing fluorescence, possibility...
The mechanical damage that often precedes the penetration of a leaf by pathogen promotes activation pectin methylesterase (PME); PME leads to emission methanol, resulting in "priming" effect on intact leaves, which is accompanied an increased sensitivity Tobacco mosaic virus (TMV) and resistance bacteria. In this study, we revealed mRNA levels methanol-inducible gene encoding Nicotiana benthamiana aldose 1-epimerase-like protein (NbAELP) leaves plants are very low compared with roots....
The introduction of a halogen or methyl substituent changes the speed flip-flop penetrating cations and anions in opposite way.
Chloramphenicol (CHL) is a ribosome-targeting antibiotic that binds to the peptidyl transferase center (PTC) of bacterial ribosome and inhibits peptide bond formation. As an approach for modifying potentially improving properties this inhibitor, we explored binding inhibitory semi-synthetic triphenylphosphonium analog CHL-CAM-C4-TPP. Our data demonstrate compound exhibits ~5-fold stronger affinity higher potency as in vitro protein synthesis inhibitor compared CHL. The X-ray crystal structure
The alkyltriphenylphosphonium (TPP) group is the most widely used vector targeted to mitochondria. Previously, length of alkyl linker was varied as well structural modifications in TPP phenyl rings obtain optimal therapeutic effect a pharmacophore conjugated with lipophilic cation. In present work, we synthesized butyltriphenylphosphonium cations halogenated and methylated (C4TPP-X) measured electrical current through planar lipid bilayer presence C4TPP-X. permeability C4TPP-X range 6 orders...
Nucleic acid aptamers are prospective molecular recognizing elements. Similar to antibodies, capable of providing specific recognition due their spatial structure. However, the apparent simplicity oligonucleotide folding is often elusive, as there a balance between several conformations and, in some cases, oligomeric structures. This research focused on establishing thermodynamic background and conformational heterogeneity taking series thrombin DNA having G-quadruplex duplex modules an...
Thechain-breaking antioxidant activities of reduced form novel type ofgeroprotectors, mitochondria-targeted quinones (QH2) havequantitatively been measured for the first time. To this end, chainperoxidation methyl linoleate (ML) in Triton micelles was used as akinetic testing model. The studied QH2 were lipophilictriphenylphosphonium cations conjugated by an aliphatic linker to anantioxidant, i.e. a ubiquinol moiety (MitoQH2) or plastoquinolmoiety (SkQH2). activity characterized bythe rate...
Nucleic acid aptamers are generally accepted as promising elements for the specific and high-affinity binding of various biomolecules. It has been shown a number that complexes with several related proteins may possess similar affinity. An outstanding example is G-quadruplex DNA aptamer RHA0385, which binds to hemagglutinins influenza A virus strains. These have homologous tertiary structures but moderate-to-low amino sequence identities. Here, experiment was inverted, targeting same protein...
The healthy human body contains small amounts of metabolic formaldehyde (FA) that mainly results from methanol oxidation by pectin methylesterase, which is active in a vegetable diet and the gastrointestinal microbiome. With age, ability to maintain low level FA decreases, increases risk Alzheimer's disease dementia. It has been shown 1,2-dithiolane-3-pentanoic acid or alpha lipoic (ALA), naturally occurring dithiol antioxidant cofactor mitochondrial α-ketoacid dehydrogenases, glutathione...