A5007261739- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Cancer therapeutics and mechanisms
- Antibiotic Resistance in Bacteria
- Telomeres, Telomerase, and Senescence
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Crystallization and Solubility Studies
- DNA and Nucleic Acid Chemistry
- Synthesis and biological activity
- X-ray Diffraction in Crystallography
- Computational Drug Discovery Methods
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Marine Sponges and Natural Products
- Antimicrobial Peptides and Activities
Institute of Bioorganic Chemistry
2018-2025
Lomonosov Moscow State University
2016-2025
Skolkovo Institute of Science and Technology
2016-2025
Moscow State University
2005-2025
Institute of Bioorganic Chemistry
2024
Physico-Technical Institute
2011-2015
Max Planck Society
2012
Max Planck Innovation
2012
Federal Medical-Biological Agency
2011
In-Q-Tel
2010
Regulation of gene expression at the level translation accounts for up to three orders magnitude in its efficiency. We systematically compared impact several mRNA features on initiation first an operon with those second gene. Experiments were done a system internal control based dual cerulean and red (CER/RFP) fluorescent proteins. demonstrated significant differences efficiency Shine Dalgarno sequences acting leading following genes operon. The majority frequent intercistronic arrangements...
This review centers on the stationary phase of bacterial culture. The basic processes specific to phase, as well regulatory mechanisms that allow bacteria survive in conditions stress, are described.
Senescence has been the focus of research for many centuries. Despite significant progress in extending average human life expectancy, process aging remains largely elusive and, unfortunately, inevitable. In this review, we attempted to summarize current theories and approaches understanding it.
Endophytic actinobacteria are one of the important pharmaceutical resources and well known for producing different types bioactive substances. Nevertheless, detection novelty, diversity bioactivity on endophytic isolated from mangrove plants scarce. In this study, five plants, Avicennia marina, Aegiceras corniculatum, Kandelia obovota, Bruguiera gymnorrhiza Thespesia populnea,were collected Beilun Estuary National Nature Reserve in Guangxi Zhuang Autonomous Region, China. One-hundred strains...
In order to accelerate drug discovery, a simple, reliable, and cost-effective system for high-throughput identification of potential antibiotic mechanism action is required. To facilitate such screening new antibiotics, we created double-reporter not only antimicrobial activity detection but also simultaneous sorting antimicrobials into those that cause ribosome stalling induce the SOS response due DNA damage. this reporter system, red fluorescent protein gene rfp was placed under control...
We have synthesized and characterized a panel of new binuclear mixed valence Cu(I,II) complexes containing substituted 2-alkylthio-5-arylmethylene-4H-imidazolin-4-ones with unusual structure. These are shown to be cytotoxic for various cell lines. found that these compounds did not intercalate DNA, inhibited number polymerases (telomerase predominantly), accumulated in the nucleus, caused DNA degradation. Preliminary studies revealed lead compound human breast adenocarcinoma growth mice model.
The ribosomal RNA (rRNA) of Escherichia coli contains 24 methylated residues. A set 22 methyltransferases responsible for modification 23 residues has been described previously. Herein we report the identification yhiR gene as encoding enzyme that modifies 23S rRNA nucleotide A2030, last whose was not known. YhiR prefers protein-free to ribonucleoprotein particles containing only part 50S subunit proteins and does methylate assembled subunit. We suggest renaming rlmJ according...
Yield of protein per translated mRNA may vary by four orders magnitude. Many studies analyzed the influence features on translation yield. However, a detailed understanding how sequence determines its propensity to be is still missing. Here, we constructed set reporter plasmid libraries encoding CER fluorescent preceded randomized 5΄ untranslated regions (5΄-UTR) and Red (RFP) used as an internal control. Each library was transformed into Escherchia coli cells, separated efficiency cell...
Abstract Amicoumacin A is an antibiotic that was recently shown to target bacterial ribosomes. It affects translocation and provides additional contact interface between the ribosomal RNA mRNA. The binding site of amicoumacin formed by universally conserved nucleotides rRNA. In this work, we showed inhibits translation in yeast mammalian systems affecting elongation. We determined structure complex with ribosomes at a resolution 3.1 Å. Toxicity measurement demonstrated human cancer cell...
Many pharmaceutical companies are avoiding the development of novel antibacterials due to a range rational reasons and high risk failure. However, there is an urgent need for antibiotics especially against resistant bacterial strains. Available in silico models suffer from many drawbacks and, therefore, not applicable scoring molecules with structural diversity by their antibacterial potency. Considering this, overall aim this study was develop efficient model able find compounds which have...
Ribosomal RNAs in all organisms are methylated. The functional role of the majority modified nucleotides is unknown. We systematically questioned influence rRNA methylation Escherichia coli on a number characteristics bacterial cells with help set methyltransferase (MT) gene knockout strains from Keio collection. Analysis ribosomal subunits sedimentation profiles revealed surprisingly small MT that significantly affected ribosome assembly. Accumulation assembly intermediates was observed...
The article analyzes modern scientific publications on plant-based meat substitutes. authors discuss a growing interest in diets and alternatives the prospects of using soy-based products. Objective. To analyze substitutes, including issues their contents, production, safety. Materials Methods. literature search analysis were conducted MedLine/PubMed, Cochrane, Google Scholar, RSCI, WHO, Food Agriculture Organization United Nations (FAO) databases for 2010—2024. Results. review shows that...
Spermatozoid’s flagella assemble in transcriptionally silent spermatids and thus depends on post-transcriptional regulation of gene expression. Mutations Nsun7 are known to cause male infertility human mice. We identified m5C-specific RNA methyltransferase as a protein present elongated interacting with RNAs specific for this type spermatozoid’s precursor cells. Inactivation mice leads upregulation its interactors, indicating that downregulates set the spermatids. A physiologic consequence...
Objective Many long noncoding RNAs (lncRNAs) are associated with liver cancers, mainly hepatocellular carcinoma (HCC) and to a smaller extent intrahepatic cholangiocarcinoma (CCA). Most of such lncRNAs show similar dysregulation patterns when the two types tumors compared, suggesting that these aberrations characteristic features tumor types. In present study, we aimed identify some candidate specifically CCA. Methods According The Cancer Genome Atlas data, chose LINC00941 , ABHD11-AS1 CASC8...
N2-Methylguanine 966 is located in the loop of Escherichia coli 16 S rRNA helix 31, forming a part P-site tRNA-binding pocket. We found yhhF to be gene encoding for m2G966 specific methyltransferase. Disruption by kanamycin resistance marker leads loss modification at G966. The could rescued expression recombinant protein from plasmid carrying gene. Moreover, purified methyltransferase, presence S-adenosylomethionine (AdoMet), able methylate 30 ribosomal subunits that were knock-out strain...
A number of ribosomal proteins in Escherichia coli undergo posttranslational modifications. Six are methylated (S11, L3, L11, L7/L12, L16, and L33), three acetylated (S5, S18, L7), protein S12 is methylthiolated. Extra amino acid residues added to S6. C-terminal partially removed from L31. The functional significance these modifications has remained unclear. These not vital the cells, it likely that they have regulatory functions. This paper reviews all known coli. Certain enzymes...