A5016453551- Gut microbiota and health
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Clostridium difficile and Clostridium perfringens research
- Reproductive tract infections research
- Mycobacterium research and diagnosis
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Bacterial Identification and Susceptibility Testing
- Tuberculosis Research and Epidemiology
- Plant Virus Research Studies
- Helicobacter pylori-related gastroenterology studies
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Bacterial Infections and Vaccines
- Inflammatory Bowel Disease
- Plant Pathogenic Bacteria Studies
- Alcohol Consumption and Health Effects
- Antibiotic Resistance in Bacteria
- Plant-Microbe Interactions and Immunity
- Aquaculture disease management and microbiota
InSysBio (Russia)
2022-2025
Ministry of Health of the Russian Federation
2000-2025
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2025
Institute for Systems Biology
2024
Moscow State University of Medicine and Dentistry
2023-2024
Institute of Bioorganic Chemistry
2011-2023
Federal Medical-Biological Agency
2012-2023
IFC Research (United Kingdom)
2023
State Scientific Center of the Russian Federation - Federal Medical Biophysical Center named after A.I. Burnazyan
2023
Moscow Institute of Physics and Technology
2011-2022
Abstract Exosomes, small (40–100 nm) extracellular membranous vesicles, attract enormous research interest because they are carriers of disease markers and a prospective delivery system for therapeutic agents. Differential centrifugation, the prevalent method exosome isolation, frequently produces dissimilar improper results faulty practice using common centrifugation protocol with different rotors. Moreover, as recommended by suppliers, adjusting duration according to rotor K-factors does...
Alcohol abuse has deleterious effects on human health by disrupting the functions of many organs and systems. Gut microbiota been implicated in pathogenesis alcohol-related liver diseases, with its composition manifesting expressed dysbiosis patients suffering from alcoholic dependence. Due to inherent plasticity, gut is an important target for prevention treatment these diseases. Identification impact alcohol associated psychiatric symptoms community structure confounded dysfunction. In...
The microbial community of the human gut has a crucial role in sustaining host homeostasis. High-throughput DNA sequencing delineated structural and functional configurations metagenomes world populations. microbiota Russian population is particular interest to researchers, because Russia encompasses uniquely wide range environmental conditions ethnogeographical cohorts. Here we conduct shotgun metagenomic analysis samples from 96 healthy adult subjects, which reveals novel structures....
Abstract Summary Phigaro is a standalone command-line application that able to detect prophage regions taking raw genome and metagenome assemblies as an input. It also produces dynamic annotated ‘prophage maps’ marks possible transposon insertion spots inside prophages. applicable for mining from large metagenomic datasets. Availability implementation Source code freely available download at https://github.com/bobeobibo/phigaro along with test data. The written in Python. Supplementary...
Autoantibody-mediated tissue destruction is among the main features of organ-specific autoimmunity. This report describes “an antibody enzyme” (abzyme) contribution to site-specific degradation a neural antigen. We detected proteolytic activity toward myelin basic protein (MBP) in fraction antibodies purified from sera humans with multiple sclerosis (MS) and mice induced experimental allergic encephalomyelitis. Chromatography zymography data demonstrated that this preparation was exclusively...
There are strong genetic components to cardiorespiratory fitness and its response exercise training. It would be useful understand the differences in genomic profile of highly trained endurance athletes world class caliber sedentary controls. An international consortium (GAMES) was established order compare elite ethnicity-matched controls a case-control study design. Genome-wide association studies were undertaken on two cohorts (GENATHLETE Japanese runners), from which panel 45 promising...
Abstract Introduction This study was aimed at deciphering the secretome of adipose-derived mesenchymal stromal cells (ADSCs) cultured in standard and hypoxic conditions to reveal proteins, which may be responsible for regenerative action these cells. Methods Human ADSCs were isolated from 10 healthy donors 3–4 passages. Cells serum deprived cell purity assessed using multiple surface markers. Conditioned media collected analyzed LC-MS with a focus on characterizing secreted proteins. Results...
Anhydrobiosis represents an extreme example of tolerance adaptation to water loss, where organism can survive in ametabolic state until returns. Here we report the first comparative analysis examining genomic background desiccation tolerance, which is exclusively found larvae only anhydrobiotic insect, Polypedilum vanderplanki. We compare genomes P. vanderplanki and a congeneric desiccation-sensitive midge nubifer. determine that genome species specifically contains clusters multi-copy genes...
Illumina's Infinium HumanMethylation450 BeadChip arrays were used to examine genome-wide DNA methylation profiles in 22 sample pairs from colorectal cancer (CRC) and adjacent tissues 19 colon tissue samples cancer-free donors. We show that the of tumors healthy can be clearly distinguished one another main source variability is associated with disease status. different statistical approaches evaluate data. In general, at CpG-site level, we found common CRC-specific patterns consist least...
Due to its rapid spread and association with the numerous outbreaks, global of East Asian lineage Mycobacterium tuberculosis strains presents a concern. Although there were many attempts describe population structure, no consensus has been reached yet. To define unbiased classification that will facilitate future studies this lineage, we analyzed performance congruence eight different genotyping schemes based on phylogenetic analysis 1,398 from 32 countries using whole-genome sequencing...
Abstract Numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of most popular topics for research. For example, outer membrane (OMVs) Bacteroides fragilis play an important nutritional role with respect other microorganisms promote anti-inflammatory effects on immune cells. However, toxigenic B. (ETBF) contributes bowel disease, even causing colon cancer. If nontoxigenic...
Ovarian cancer ascites is a native medium for cells that allows investigation of their secretome in natural environment. This interest as promising source potential biomarkers, and also cell–cell communication. The aim this study was to elucidate specific features the malignant metabolome proteome. In order omit components systemic response formation, we compared with cirrhosis ascites. Metabolome analysis revealed 41 differed significantly between Most identified cancer-specific metabolites...
Abstract Whole-exome sequencing is an attractive alternative to microarray analysis because of the low cost and potential ability detect copy number variations (CNV) various sizes (from 1–2 exons several Mb). Previous comparison most popular CNV calling tools showed a high portion false-positive calls. Moreover, due lack gold standard set, results are limited incomparable. Here, we aimed perform comprehensive capable germline available at moment using single reference sample set. Compiling...
Tuberculosis (TB) poses a worldwide threat due to advancing multidrug-resistant strains and deadly co-infections with Human immunodeficiency virus. Today large amounts of Mycobacterium tuberculosis whole genome sequencing data are being assessed broadly yet there exists no comprehensive online resource that connects M. variants geographic origin, drug resistance or clinical outcome. Here we describe inclusive unifying Genome-wide Variation (GMTV) database, ( http://mtb.dobzhanskycenter.org )...
To investigate the association between multiple single-nucleotide polymorphisms (SNPs), aerobic performance and elite endurance athlete status in Russians. By using GWAS approach, we examined 1,140,419 SNPs relative maximal oxygen consumption rate ([Formula: see text]O2max) 80 international-level Russian athletes (46 males 34 females). validate obtained results, further performed case-control studies by comparing frequencies of most significant (with P < 10(-5)-10(-8)) 218 opposite cohorts...
Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success FTM in rCDI led to a number studies investigating effectiveness its application other gastrointestinal diseases. However, majority effects FMT were evaluated on patients with initially altered microbiota. The aim our study was estimate gut composition healthy volunteers and monitor long-term outcomes.We have performed combined analysis three...
Genomes contain millions of short (<100 codons) open reading frames (sORFs), which are usually dismissed during gene annotation. Nevertheless, peptides encoded by such sORFs can play important biological roles, and their impact on cellular processes has long been underestimated. Here, we analyzed approximately 70,000 transcribed in the model plant Physcomitrella patens (moss). Several distinct classes that differ terms position transcripts level evolutionary conservation present moss...
Background. The ongoing pandemic of the novel coronavirus infection (COVID-19) draws attention to significance molecular and genetic monitoring SARS-CoV-2 spread among population Russian Federation. aim study was analyze dynamics circulation variants in Russia.Materials methods. analysis for Russia carried out, covering period from 28/12/2020 26/6/2022. included data Rospotrebnadzor Report No. 970 "Information about Infectious Diseases Individuals with Suspected Novel Coronavirus Infection"...
The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has significantly impacted global healthcare, underscoring importance of exploring virus's effects on infected individuals beyond treatments and vaccines. Notably, recent findings suggest that can infect gut, thereby altering gut microbiota. This study aimed to analyze microbiota composition differences between patients experiencing mild severe symptoms. We conducted 16S rRNA metagenomic sequencing fecal samples from 49 43 cases upon...