A5084958731- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Liver physiology and pathology
- Proteoglycans and glycosaminoglycans research
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
- Cancer-related gene regulation
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Escherichia coli research studies
- RNA Research and Splicing
- Bacterial biofilms and quorum sensing
- Cancer, Hypoxia, and Metabolism
- Fibroblast Growth Factor Research
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Neonatal Respiratory Health Research
- Biochemical and Molecular Research
- Porphyrin Metabolism and Disorders
- Enzyme Structure and Function
- Liver Disease Diagnosis and Treatment
- Photoacoustic and Ultrasonic Imaging
Skolkovo Institute of Science and Technology
2018-2024
Lomonosov Moscow State University
2011-2015
Moscow State University
2011
Physico-Technical Institute
2011
A number of ribosomal proteins in Escherichia coli undergo posttranslational modifications. Six are methylated (S11, L3, L11, L7/L12, L16, and L33), three acetylated (S5, S18, L7), protein S12 is methylthiolated. Extra amino acid residues added to S6. C-terminal partially removed from L31. The functional significance these modifications has remained unclear. These not vital the cells, it likely that they have regulatory functions. This paper reviews all known coli. Certain enzymes...
A new promising trend in personalized medicine is the use of autologous cells (macrophages or stem cells) for cell-based therapy and also as a "Trojan horse" targeted delivery drug carrier. The natural ability macrophages chemotaxis allows them to deliver cargo damaged area, significantly reducing side effects on healthy organ tissues. Therefore, it important develop tools track their behavior organism. While labeled containers can serve anchored tags imaging vivo, they affect properties...
4-methylumbelliferone (4MU) is a well-known hyaluronic acid synthesis inhibitor and an approved drug for the treatment of cholestasis. In animal models, 4MU decreases inflammation, reduces fibrosis, lowers body weight, serum cholesterol, insulin resistance. It also inhibits tumor progression metastasis. The broad spectrum effects suggests multiple yet unknown targets 4MU. Aiming at target deconvolution, we have analyzed publicly available data bases, including: 1. Small molecule library Bio...
Abstract Due to breakthroughs in RNAi and genome editing methods the past decade, it is now easier than ever study fine details of protein synthesis animal models. However, most our understanding translation comes from unicellular organisms cultured mammalian cells. In this study, we demonstrate feasibility perturbing a mouse liver by targeting elongation factor 2 (eEF2) with RNAi. We were able achieve over 90% knockdown efficacy maintain for weeks effectively slowing down rate elongation....
Modification of ribosomal RNA is ubiquitous among living organisms. Its functional role well established for only a limited number modified nucleotides. There are examples rRNA modification involvement in the gene expression regulation cell. need large data set analysis search potential partners modification. In this study, we extracted phylogenetic profile, genome neighbourhood, co-expression and phenotype profile co-purification regarding Escherichia coli enzymes from public databases....
Abstract Hepatic fibrosis remains a significant clinical challenge due to ineffective treatments. 4-methylumbelliferone (4MU), hyaluronic acid (HA) synthesis inhibitor, has proven safe in phase one trials. In this study, we aimed ameliorate liver by inhibiting HA synthesis. We compared two groups of mice with CCl 4 -induced fibrosis, treated (4MU) and hyaluronan synthase 2 (HAS2) targeting siRNA (siHAS2). The administration 4MU siHAS2 significantly reduced collagen deposition, as well...
Microcin C (McC) is a peptide-nucleotide antibiotic produced by Escherichia coli cells harboring plasmid-borne operon mccABCDE.The heptapeptide MccA converted into McC adenylation catalyzed the MccB enzyme.Since substrate for MccB, mechanism that regulates MccA/MccB ratio likely exists.Here, we show transcription from promoter located upstream of mccA directs synthesis two transcripts: short highly abundant transcript containing ORF and longer minor downstream ORFs.The generated when RNA...
Abstract Elevated plasma levels of hyaluronic acid (HA) is a disease marker in liver pathology and other inflammatory disorders. Inhibition HA synthesis with coumarin 4-methylumbelliferone (4MU) has beneficial effect animal models fibrosis, inflammation, cancer metabolic syndrome. 4MU an active compound approved choleretic drug hymecromone low bioavailability broad spectrum action. New, more specific efficient inhibitors hyaluronan synthases (HAS) are required. We have tested several newly...
Autologous macrophage transfer is an emerging platform for cell therapy. It anticipated that conventional reprogramming based on ex vivo polarization using cytokines and ligands of TLRs may enhance the therapeutic effect. We describe alternative approach small interfering RNA (siRNA) knockdown selected molecular cues polarization, namely EGR2, IRF3, IRF5, TLR4 in Raw264.7 monocyte/macrophage line mouse-bone-marrow-derived macrophages (BMDMs). The impact IRF5 was most pronounced, curtailing...
In bacteria, rapid adaptation to changing environmental conditions depends on the interplay between housekeeping and alternative σ factors, responsible for transcription of specific regulons by RNA polymerase (RNAP). comparison with primary σs contain poorly conserved region 1.1, whose functions in are only partially understood. We found that a single mutation 1.1 Escherichia coli σ70 rewires regulation during cell growth resulting profound phenotypic changes. Despite its destabilizing...