A5088876382- Extracellular vesicles in disease
- Photoacoustic and Ultrasonic Imaging
- Photonic and Optical Devices
- Analytical Chemistry and Sensors
- Nanoparticle-Based Drug Delivery
- Advanced Biosensing Techniques and Applications
- Nanoplatforms for cancer theranostics
- Lattice Boltzmann Simulation Studies
- Nanopore and Nanochannel Transport Studies
- Ultrasound and Hyperthermia Applications
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
- Lanthanide and Transition Metal Complexes
- Thermal properties of materials
- Gold and Silver Nanoparticles Synthesis and Applications
- Biosensors and Analytical Detection
- Phase Equilibria and Thermodynamics
- Advanced biosensing and bioanalysis techniques
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Polymer Surface Interaction Studies
- Intracerebral and Subarachnoid Hemorrhage Research
- Electrical and Bioimpedance Tomography
- Ultrasound and Cavitation Phenomena
- Advanced Fiber Optic Sensors
Skolkovo Institute of Science and Technology
2017-2025
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation
2023-2025
Moscow Institute of Physics and Technology
2018-2022
Lomonosov Moscow State University
2021
University of Utah
2006-2015
CellProfiler has enabled the scientific research community to create flexible, modular image analysis pipelines since its release in 2005. Here, we describe 3.0, a new version of software supporting both whole-volume and plane-wise three-dimensional (3D) stacks, increasingly common biomedical research. CellProfiler's infrastructure is greatly improved, provide protocol for cloud-based, large-scale processing. New plugins enable running pretrained deep learning models on images. Designed by...
Exosomes and other extracellular vesicles (EVs) are molecular complexes consisting of a lipid membrane vesicle, its surface decoration by proteins molecules, diverse luminal content inherited from parent cell, which includes RNAs, proteins, DNAs. The characterization the hydrodynamic sizes EVs, depends on size vesicle coronal layer formed decorations, has become routine. For exosomes, smallest relative difference between is significant. cryogenic transmission electron microscopy (cryo-TEM)...
Abstract We developed a novel asymmetric depth filtration (DF) approach to isolate extracellular vesicles (EVs) from biological fluids that outperforms ultracentrifugation and size‐exclusion chromatography in purity yield of isolated EVs. By these metrics, single‐step DF matches or exceeds the performance multistep protocols with dedicated purification procedures isolation plasma demonstrate selective transit capture nanoparticles pores by size elasticity, low surface binding medium, ability...
The concept of liquid biopsy has emerged as a novel approach for cancer screening, which is based on the analysis circulating biomarkers in body fluids. Among various biomarkers, including Food and Drug Administration (FDA)-approved tumor cells (CTC) DNA (ctDNA), exosomes have attracted tremendous attention due to their ability diagnose its early stages with high efficiency. Recently, surface-enhanced Raman spectroscopy (SERS) been applied detection sensitivity, specificity, multiplexing...
Alzheimer’s disease (AD) is one of the most common progressive neurodegenerative diseases leading to impairments in memory, orientation, and behavior. However, significant work still needed fully understand progression such develop novel therapeutic agents for AD prevention treatment. Small extracellular vesicles (sEVs) have received attention recent years due their potential effects on AD. The aim this study was determine effect sEVs an vitro model were isolated from human Wharton’s jelly...
Microbubbles have already reached clinical practice as ultrasound contrast agents for angiography. However, modification of the bubbles’ shell is needed to produce probes and multimodal (fluorescence/photoacoustic) imaging methods in combination with theranostics (diagnostics therapeutics). In present work, hybrid structures based on microbubbles an air core a composed bovine serum albumin, albumin-coated gold nanoparticles, clinically available photodynamic dyes (zinc phthalocyanine,...
Photonic biosensors based on photonic integrated circuits (PICs) and microfluidic channels (MFCs) have become the subject of intensive research for point-of-care (POC) device applications. In presented work, we demonstrate possibility identifying complex refractive index (RI) analyzed liquids through optimization geometry configuration MFCs under PICs by experimental numerical approaches. Our results suggest that real imaginary parts RI analytes study can be determined from spectrum devices...
The isolation of small extracellular vesicles (sEVs), including those secreted by pathological cells, with high efficiency and purity is highly demanded for research studies practical applications. Conventional sEV methods suffer from low yield, presence contaminants, long-term operation costs. Bead-assisted platforms are considered to be effective trapping sEVs recovery yield sufficient further molecular profiling. In this study, magnetically responsive beads made calcium carbonate (CaCO3)...
Fluorocarbons are highly hydrophobic, biocompatible compounds with a variety of medical applications. Despite significant interest, the study interfacial properties fluorocarbons in aqueous systems has received limited attention. In this study, we investigate influence perfluoropentane and perfluorohexane vapors on surface tension water at room temperature. The results show substantial decrease presence perfluorocarbon vapors. investigated range partial pressures up to saturation value,...
Today, a lab-on-a-chip is one of the most promising ways to create sensor devices for gas and liquid analysis environmental monitoring, early diagnosis, treatment effectiveness assessment. On hand, this requires large number measurements and, on other involves minimum consumption test analytes. Combination highly sensitive photonic integrated circuits (PICs) with microfluidic channels (MFCs) necessary solve problem. In work, PICs based silicon nitride platform MFCs studying liquids gases...
Microbubbles are routinely used ultrasound contrast agents in the clinic. While a soft protein shell is commercially preferable for imaging purposes, rigid polymer demonstrates prolonged agent stability. Hence, combining polymers and proteins one composition can advance microbubble properties. We formulated hybrid "protein–copolymer" with complex of bovine serum albumin an amphiphilic copolymer N-vinyl-2-pyrrolidone acrylic acid. The resulting microbubbles demonstrated advanced...
Liquid/surfactant/gas interfaces are promising objects for nanoengineered multimodal contrasts, which can be used biomedical imaging in preclinical and clinical applications. Microbubbles with the gaseous core shell made of lipids/proteins have already acted as ultrasound (US) contrast agents angiography. In present work, microbubbles a composed Span 60 Tween 80 surfactants functionalized fluorescein isothiocyanate gold nanorods to achieve combination US, fluorescence, optoacoustic...
Today, the search for disease biomarkers and techniques their detection is one of most important focuses in modern healthcare. Extracellular vesicles (EVs) are known to be related pathogenesis various illnesses, such as cancer, neurodegenerative disease, cardiovascular disease. Specific EV potential control amount biological fluids can provide a promising therapeutic strategy that involves reduction production circulation normal levels prevent progression. To foundation research development,...
The microfluidic approach enables both to assemble the structured silica monolayer patterns atop photonic integrated chips and apply them as optical gas sensors.