A5065320376- Chemical Synthesis and Analysis
- Mitochondrial Function and Pathology
- DNA and Nucleic Acid Chemistry
- Molecular Sensors and Ion Detection
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Nerve Injury and Rehabilitation
- Advanced biosensing and bioanalysis techniques
- Bioactive Compounds and Antitumor Agents
- Synthesis and Characterization of Heterocyclic Compounds
- Biochemical effects in animals
- RNA Interference and Gene Delivery
- Free Radicals and Antioxidants
- Photoreceptor and optogenetics research
- Pain Management and Treatment
- Carbohydrate Chemistry and Synthesis
- Neuropeptides and Animal Physiology
- Coenzyme Q10 studies and effects
- Fluorine in Organic Chemistry
- Neuroscience and Neuropharmacology Research
- Neurological Disorders and Treatments
- Orthopaedic implants and arthroplasty
- Bee Products Chemical Analysis
Lomonosov Moscow State University
2014-2024
Saratov State Medical University
2017-2023
Moscow State University
1990-2023
Candid
2022
Kuban State Agrarian University
2022
Central Scientific Research Institute of Traumatology and Orthopedics
2022
Materials Technology (United Kingdom)
2022
University of Philosophical Research
2022
St Petersburg University
2022
Saratov State University
2018-2019
Caffeic acid phenethyl ester, an active component of propolis extract, inhibits 5-lipoxygenase in the micromolar concentration range. The inhibition is uncompetitive type, i.e. inhibitor binds to enzyme-substrate complex but not free enzyme. ester also exhibits antioxidant properties. At a 10 microM, it completely blocks production reactive oxygen species human neutrophils and xanthine/xanthine oxidase system.
A unique phenomenon of mitochondria-targeted protonophores is described. It consists in a transmembrane H + -conducting fatty acid cycling mediated by penetrating cations such as 10-(6’-plastoquinonyl)decyltriphenylphosphonium (SkQ1) or dodecyltriphenylphosphonium (C 12 TPP). The has been modeled molecular dynamics and directly proved experiments on bilayer planar phospholipid membrane, liposomes, isolated mitochondria, yeast cells. In the concerted action acids found to result conversion pH...
A limited decrease in mitochondrial membrane potential can be beneficial for cells, especially under some pathological conditions, suggesting that mild uncouplers (protonophores) causing such an effect are promising candidates therapeutic uses. The great majority of protonophores weak acids capable permeating across membranes their neutral and anionic forms. In the present study, protonophorous activity a series derivatives cationic rhodamine 19, including dodecylrhodamine (C(12)R1) its...
Mitochondria-targeted antioxidants are known to alleviate mitochondrial oxidative damage that is associated with a variety of diseases. Here, we showed SkQ1, decyltriphenyl phosphonium cation conjugated quinone moiety, exhibited strong antibacterial activity towards Gram-positive Bacillus subtilis, Mycobacterium sp. and Staphylococcus aureus Gram-negative Photobacterium phosphoreum Rhodobacter sphaeroides in submicromolar micromolar concentrations. SkQ1 less antibiotic Escherichia coli due...
We explored the neuroprotective properties of natural plant-derived antioxidants plastoquinone and thymoquinone (2-demethylplastoquinone derivative) modified to be specifically accumulated in mitochondria. The modification was performed through chemical conjugation quinones with penetrating cations: Rhodamine 19 or tetraphenylphosphonium. Neuroprotective were evaluated a model middle cerebral artery occlusion. demonstrate that mitochondria-targeted compounds, introduced immediately after...
Multiple sclerosis (MS) is one of the most widespread chronic neurological diseases that manifests itself by progressive demyelination in central nervous system. The study MS pathogenesis begins with onset relapsing-remitting phase disease, which becomes apparent due to microglia activation, neuroinflammation and demyelination/ remyelination white matter. following accompanied severe symptoms when neurodegeneration are spread both gray In this review, we discuss a possible role mitochondrial...
The mitochondrial membrane potential (∆Ψ) is the driving force providing electrical component of total transmembrane hydrogen ions generated by proton pumps, which utilized ATP synthase. role ∆Ψ not limited to its in bioenergetics since it takes part other important intracellular processes, leads mandatory requirement homeostasis ∆Ψ. Conventionally, living cells estimated fluorescence probes such as rhodamine 123, tetramethylrodamine, etc. However, when assessing fluorescence, possibility...
This study assesses a protective effect of mitochondria-targeted antioxidant SkQT1 (a mixture 10-(6'-toluquinonyl) decyltriphenylphosphonium and 10-(5'-toluquinonyl) in proportion 1.4:1), using an open focal trauma model the rat brain sensorimotor cortex amyloid-beta1-42 (Abeta)-induced impairment hippocampal long-term potentiation (LTP), kind synaptic plasticity associated with learning memory. It was found that trauma-induced neurological deficit could be partially improved daily...
Linking decyl-triphenyl-phosphonium to fluorescein yields a fluorescent probe that accumulates in energized mitochondria, facilitates proton transfer across membranes and stimulates mitochondrial respiration. This features mitochondria-targeted uncoupler, being of potential interest for therapeutic use against oxidative stress-related diseases.
The introduction of a halogen or methyl substituent changes the speed flip-flop penetrating cations and anions in opposite way.
Appending a lipophylic alkyl chain by ester bond to fluorescein has been previously shown convert this popular dye into an effective protonophoric uncoupler of oxidative phosphorylation in mitochondria, exhibiting neuro- and nephroprotective effects murine models. In line with finding, we here report data on the pronounced depolarizing effect series decyl esters bacterial cells. The binding derivatives Bacillus subtilis cells was monitored fluorescence microscopy correlation spectroscopy...
The alkyltriphenylphosphonium (TPP) group is the most widely used vector targeted to mitochondria. Previously, length of alkyl linker was varied as well structural modifications in TPP phenyl rings obtain optimal therapeutic effect a pharmacophore conjugated with lipophilic cation. In present work, we synthesized butyltriphenylphosphonium cations halogenated and methylated (C4TPP-X) measured electrical current through planar lipid bilayer presence C4TPP-X. permeability C4TPP-X range 6 orders...