A5063099776- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Photosynthetic Processes and Mechanisms
- Single-cell and spatial transcriptomics
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Cell Image Analysis Techniques
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Microfluidic and Bio-sensing Technologies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Cellular transport and secretion
- Algal biology and biofuel production
- Cancer-related Molecular Pathways
- Extracellular vesicles in disease
- Molecular Biology Techniques and Applications
- Cancer Treatment and Pharmacology
- 14-3-3 protein interactions
Lomonosov Moscow State University
2014-2025
Astana Medical University
2024-2025
Nazarbayev University
2016-2025
National Research Center for Maternal and Child Health
2023
Moscow State University
1996-2018
Center for Theoretical Problems of Physicochemical Pharmacology
2018
Boston Children's Hospital
2012-2015
Harvard University
2012-2015
National Research Center for Hematology Russian Academy of Medical Sciences
2003-2014
Chinese University of Hong Kong
2014
CLIP-associating protein (CLASP) 1 and CLASP2 are mammalian microtubule (MT) plus-end binding proteins, which associate with CLIP-170 CLIP-115. Using RNA interference in HeLa cells, we show that the two CLASPs play redundant roles regulating density, length distribution stability of interphase MTs. In both concentrate on distal MT ends a narrow region at cell margin. stabilize MTs by promoting pauses restricting growth shortening episodes to this peripheral region. We demonstrate middle part...
A study was made of the structure centrosome in cell cycle a nonsynchronous culture pig kidney embryo (PE) cells. In spindle pole metaphase there are two mutually perpendicular centrioles (mother and daughter) which differ their ultrastructure. An electron-dense halo, surrounds only mother centriole is site where microtubules converge, disappears at end telophase. anaphase, situated to axis. At beginning G1 period, pericentriolar satellites formed on with attached them; diverge. The...
Microtubule dynamics were investigated in CHO and NRK cells by novel experimental approaches designed to evaluate the microtubule behavior cell interior. These were: (1) laser photobleaching of a path through centrosome; (2) direct observation microtubules centrosome-containing cytoplasts; (3) GFP-CLIP-170 expression as marker for plus end growth; (iv) sequential subtraction analysis. The combination these allowed us obtain data where density had previously prevented conventional methods be...
Imaging flow cytometry (IFC) captures multichannel images of hundreds thousands single cells within minutes. IFC is seeing a paradigm shift from low- to high-information-content analysis, driven partly by deep learning algorithms. We predict wealth applications with potential translation into clinical practice. combines the high-throughput, multiparameter capabilities conventional morphological and spatial information, all at single-cell resolution. Multichannel digital individual can be...
The ends of growing microtubules (MTs) accumulate a set diverse factors known as MT plus end–tracking proteins (+TIPs), which control microtubule dynamics and organization. In this paper, we identify SLAIN2 key component +TIP interaction networks. We showed that the C-terminal part bound to end-binding (EBs), cytoplasmic linker (CLIPs), CLIP-associated characterized in detail with EB1 CLIP-170. Furthermore, found N-terminal interacted ch-TOG, mammalian homologue polymerase XMAP215. Through...
DNA methylation profiling has become an important aspect of biomedical molecular analysis. Polymerase chain reaction (PCR) amplification bisulphite-treated is a processing step that common to many currently used methods quantitative Preferential unmethylated alleles—known as PCR-bias—may significantly affect the accuracy quantification. To date, no universal experimental approach been reported overcome problem. This study presents effective method correcting biased data. The procedure...
Wound healing assay performed with automated microscopy is widely used in drug testing, cancer cell analysis, and similar approaches. It easy to perform, the results are reproducible. However, it usually as a semi-quantitative approach because of inefficient image segmentation transmitted light microscopy. Recently, several algorithms for wound quantification were suggested, but none them was tested on large dataset. In current study, we develop pipeline allowing achieve correct edges...
ABSTRACT The origin of non-centrosomal microtubules was investigated in a variety animal cells culture by means time-lapse digital fluorescence microscopy. A previous study (Keating et al. (1997)Proc. Nat. Acad. Sci. USA 94, 5078-5083) demonstrated pathway for formation release from the centrosome. Here we show parallel not dependent upon Correlative immunostaining with anti-tubulin antibodies and electron microscopy established that apparent free observed vivo were growing ends long stable...
This study comprises the comprehensive toxicological assessment of thiolated organosilica nanoparticles (NPs) synthesised from 3-mercaptopropyltrimethoxysilane (MPTS). We investigated influence three different types 3-mercaptopropyltrimethoxysilane: starting silica (Si-NP-SH) and their derivatives prepared by surface PEGylation with PEG 750 (Si-NP-PEG750) 5000 Da (Si-NP-PEG5000m) on biological subjects in vitro to vivo experiments explore possible applications those biomedical research. As a...
Tubulins are among the most successful targets for cancer chemotherapy. However, emergence of drug resistance stimulates continuous search novel chemotherapeutics. Here, we discover that coumarin-30, a widely available laser dye, binds to colchicine site tubulin and inhibits microtubule dynamics cell division at submicromolar concentrations. By combining coumarin-30 as fluorescent probe with microscale thermophoresis approach, develop versatile assay detecting tubulin–ligand interactions...
Tubulins are among the most successful targets for cancer chemotherapy. However, emergence of drug resistance stimulates continuous search novel chemotherapeutics. Here, we discover that coumarin-30, a widely available laser dye, binds to colchicine site tubulin and inhibits microtubule dynamics cell division at submicromolar concentrations. By combining coumarin-30 as fluorescent probe with microscale thermophoresis approach, develop versatile assay detecting tubulin–ligand interactions...
Polarized radial arrays of cytoplasmic microtubules (MTs) with minus ends clustered at the cell center define organization cytoplasm through interaction microtubule motors bound to membrane organelles or chromosomes. It is generally assumed that results from nucleation MTs centrosome. However, MT array can also be attained self-organization requires activity a minus-end-directed motor, dynein. In this study we examine role dynein in fragments fish melanophores lacking After activation...
ABSTRACT Turnover is important for the maintenance and remodeling of cytoskeleton during processes cell morphogenesis, mitosis motility. Microtubule (MT) turnover thought to occur by dynamic instability, growth shortening at distal (plus) ends. Recent observation MT release from centrosome depolymerization proximal (minus) ends indicates existence a minus end pathway. To evaluate relative contributions plus pathways turnover, we analyzed dynamics in model system, fish melanophore, large...
Abstract Epithelial cells undergoing EMT experience significant alterations at transcriptional and morphological levels. However, changes in the cytoskeleton, especially cytoskeleton dynamics are poorly described. Addressing question we induced three cell lines (MCF-7, HaCaT A-549) analyzed cytoskeletal there using immunostaining life imaging of transfected with microtubule focal adhesion markers. In all studied lines, area after increased, MCF-7 A-549 became elongated, while kept aspect...
Irradiation of the mitotic (prophase and prometaphase) tissue culture PK (pig kidney embryo) cells using mercury arc lamp band-pass filters postponed or inhibited anaphase onset. The biological responses observed after irradiation were: (i) normal cell division, (ii) delay in metaphase then incomplete cytokinesis, (iii) exit into interphase without separation chromosomes, (iv) complete blockage. Cell sensitivity to light at wavelengths from 423 488 nm was nearly same; near UV (wavelength 360...