A5004897328- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Organometallic Compounds Synthesis and Characterization
- Metal complexes synthesis and properties
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- MicroRNA in disease regulation
- Plant Reproductive Biology
- Polymer Surface Interaction Studies
- Polysaccharides Composition and Applications
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Edible Oils Quality and Analysis
- Bacteriophages and microbial interactions
- Botanical Research and Applications
- Light effects on plants
- Phytochemicals and Antioxidant Activities
- Bone Tissue Engineering Materials
- Extracellular vesicles in disease
- Cancer, Lipids, and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Biocrusts and Microbial Ecology
- Nuclear Receptors and Signaling
- Protist diversity and phylogeny
National Research Council
2009-2025
Instituto di Biofisica
2013-2023
Washington State University
2019
Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy
2003-2011
Universidade Estadual de Maringá
2007
University of Palermo
1990-1994
In the last decades, plant extracts have received great attention. particular, many studies pointed out potential neuroprotective effect of polyphenols-rich from plants. Evidence indeed highlights action polyphenols, both as antioxidants and inhibitors in formation amyloid protein aggregates, known to be involved neurodegenerative diseases. this work, aqueous obtained at high room temperature leaves Moringa oleifera (MO) harvested Sicily were characterized for polyphenolic content,...
Human ageing can be characterized by a profile of circulating microRNAs (miRNAs), which are potentially predictors biological age. They used as biomarker risk for age-related inflammatory outcomes, and senescent endothelial cells (ECs) have emerged possible source miRNAs. In this paper, panel four miRNAs including miR-146a-5p, miR-126-3p, miR-21-5p, miR-181a-5p, involved in several pathways related to inflammation, ECs senescence that seem characteristic the healthy phenotype. The levels...
Ginger (Zingiber officinale) is commonly consumed as spice or herbal medicine with anti-inflammatory, antioxidant, and anticancer properties. It rich of many bioactive constituents, mainly gingerols shogaols. Although the constituents have been identified, molecular mechanisms ginger action are still limited, related signalling pathways not completely defined. Here, we used a simple ethanol/freeze-drying method to obtain new extract (GE), which was chemically characterised by...
A 659 bp cDNA clone 1 coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The insert showed open reading frame 429 allergenic protein 14,866 Da deduced amino acid containing 143 residues. expressed recombinant represented the major Par j I since it reacted with 95% sera Pj‐allergic patients ( n = 22) two I‐specific monoclonal antibodies. No similarity other known sequences detected.
Amphiphilic cyclodextrin (ACyD) provides water-soluble and adaptable nanovectors by modulating the balance between hydrophobic hydrophilic chains at both CyD sides. This work aimed to design nanoassemblies based on nonionic ACyD (SC6OH) for delivery of a poor-water-soluble organotin(IV)-porphyrin derivative [(Bu3Sn)4TPPS] melanoma cancer cells. To characterize porphyrin derivatives under simulated physiological conditions, speciation was performed using complementary techniques. In aqueous...
A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using pool 23 sera Pj‐allergic patients. The contained insert 622 nucleotides with open reading frame 133 amino acids (aa) putative signal peptide 31 aa giving deduced mature processed protein 102 molecular mass 11 344 Da. expressed recombinant protein, named rPar j 2.0101, was major since it reacted IgE 82% (23/28) the subjects analyzed. It shown to be new...
Abstract Par j 1.0101 is one of the two major allergens Parietaria judaica (Pj) pollen, and its three-dimensional structure was built by structural homology modeling. The resultant model used to identify putative IgE binding regions. Western blot analysis gene fragmentation products showed that 1 30 region capable specific from a pool sera (n = 30) patients allergic Pj pollen. Using as guide, deletion site-directed mutagenesis performed, amino acids involved in were identified. In addition,...
Two cDNA clones named P9* and P1* of 794 631 bp, respectively, were isolated from a lambda ZAP expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies pool sera (n = 23) patients allergic to Pj. Sequence analysis showed open reading frames 176 138 amino acids. Both contain putative signal peptide giving two mature processed proteins Par j 1.0102 14,726 D 1.0201 10,677 D. These represent isoallergenic forms the major Pj allergen 1.0101 (clone P5) previously reported....
Parietaria pollen is one of the major cause pollinosis in southern Europe. Specific immunotherapy only treatment able to modify natural outcome disease restoring a normal immunity against allergens.We designed recombinant molecule (PjEDloop1) comprised genetic-engineered variants allergens (Par j 2/Par 1). Purity and chemical-physical properties derivative were analysed by RP-HPLC chromatography Photon Correlation Spectroscopy. Immunological activity was evaluated means Western blotting,...
Fabry disease (FD) is a lysosomal storage disorder (LSD) characterized by accumulation of glycosphingolipids in wide variety cytotypes, including endothelial cells (ECs). FD patients experience significantly reduced life expectancy compared to the general population; therefore, association with premature aging process would be plausible. To assess this hypothesis, miR-126-3p, senescence-associated microRNA (SA-miRNAs), was considered as an biomarker. The levels miR-126-3p contained small...
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels different dimensions for purposes, is described. Preparation such as easy agarose gel preparation, polymerized are used to fractionate proteins or small DNA fragments using common electrophoretic tank. This was electrophoretically immuno-blot analyses, particularly in the study allergenic response Parietaria judaica pollen senescence, Southern-blot hybridizations polymorphisms.
Bryophytes comprise of the mosses, liverworts, and hornworts. Cryphaea heteromalla, (Hedw.) D. Mohr, is a non-vascular lower plant belonging to mosses group. To date, most chemically characterized species belong while only 3.2% 8.8% hornworts, respectively, have been investigated. In this work, we present Folin-Ciocalteu oxygen radical absorbance capacity (ORAC) data related crude extracts C. heteromalla obtained by three different extraction solvents: pure water (WT), methanol:water (80:20...
Pollen allergens are multivalent proteins that cross‐link IgE antibodies on mast or basophil cells, inducing secretion of biologic mediators, and resulting in various allergic symptoms. The IgE‐binding regions the Parietaria judaica ( Pj ) pollen major allergen rPar j 2 were investigated. Twenty‐nine single sera from ‐allergic subjects tested by Western blot against five recombinant peptides. At least four putative epitopes identified. analysis their diffusion suggested a heterogeneous...