A 659 bp cDNA clone 1 coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The insert showed open reading frame 429 allergenic protein 14,866 Da deduced amino acid containing 143 residues. expressed recombinant represented the major Par j I since it reacted with 95% sera Pj‐allergic patients ( n = 22) two I‐specific monoclonal antibodies. No similarity other known sequences detected.

Static RAM-based field programmable gate arrays (SRAM-based FPGAs) are widely adopted in trigger and data acquisition systems of high-energy physics detectors for implementing fast logic due to their reconfigurability, large real-time processing capabilities embedded high-speed serial IOs. These devices sensitive radiation-induced upsets, which may alter the functionality implemented circuit. Presently, usage on-detector is limited there a strong interest finding solutions improving...

A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using pool 23 sera Pj‐allergic patients. The contained insert 622 nucleotides with open reading frame 133 amino acids (aa) putative signal peptide 31 aa giving deduced mature processed protein 102 molecular mass 11 344 Da. expressed recombinant protein, named rPar j 2.0101, was major since it reacted IgE 82% (23/28) the subjects analyzed. It shown to be new...

Abstract Par j 1.0101 is one of the two major allergens Parietaria judaica (Pj) pollen, and its three-dimensional structure was built by structural homology modeling. The resultant model used to identify putative IgE binding regions. Western blot analysis gene fragmentation products showed that 1 30 region capable specific from a pool sera (n = 30) patients allergic Pj pollen. Using as guide, deletion site-directed mutagenesis performed, amino acids involved in were identified. In addition,...

In the past years, precise measurements of time intervals have been realized using methods such as time- stretching, Vernier and delay line. this paper, we present two high-resolution time-interval measuring system implemented in a SRAM-based FPGA device. The ought to be used for interpolation within clock cycle. first method, dedicated carry lines are perform fine measurement, while second one differential tapped line is used. paper compare architectures show their performance terms...

Digitally-controlled delay lines (DCDLs) play a key role in timing distribution for trigger and data acquisition systems (TDAQ) of high energy Physics (HEP), where it is often necessary to add an open-loop fine-grained programmable phase distributed clocks and/or lines. In this work, we present the performance DCDLs implemented according all-digital novel architecture. The architecture completely technology-independent, described by means hardware description language can be placed routed...

We investigated the capacitance of a forward-biased silicon pn diode using impedance spectroscopy. Despite extensive research spanning decades, no single model in literature adequately describes behavior for bias up to built-in voltage. By employing 1N4007 as case study, we analyzed over wide frequency range, from 1 Hz MHz. Our analysis reveals that can be effectively studied by combining two models. In both models, depletion is assumed an ideal capacitor with value independent frequency....

Two cDNA clones named P9* and P1* of 794 631 bp, respectively, were isolated from a lambda ZAP expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies pool sera (n = 23) patients allergic to Pj. Sequence analysis showed open reading frames 176 138 amino acids. Both contain putative signal peptide giving two mature processed proteins Par j 1.0102 14,726 D 1.0201 10,677 D. These represent isoallergenic forms the major Pj allergen 1.0101 (clone P5) previously reported....

High-speed serial links are a key component of data acquisition systems for High Energy Physics. They carry physics events and often also clock, trigger fast control signals. For the latter applications, jitter on clock recovered from stream is critical parameter since it directly affects timing performance systems. Latest Field Programmable Gate Arrays (FPGAs) include multi-gigabit transceivers, which configurable with various options support many encodings. However, an in-depth...

Fixed-latency serial links find application in trigger and data acquisition systems of high energy physics (HEP) experiments requiring a predictable transfer timing. In some architectures, there is the need to clock out from link synchronously with system (i.e., synchronous transfers) instead using recovered stream. this work, we present architecture based on high-speed transceivers embedded latest generation field programmable gate arrays (FPGAs). These are typically designed for...

Dense wavelength division multiplexing (DWDM) is an optical technology that allows transmitting across a fiber many wavelengths, which can be added and dropped by means of passive components. We present discuss the performance complex DWDM network data transmission system will find application in NEMO underwater neutrino telescope. The tests cover qualification complete multipoint consisting transponders, fibers, filters, amplifier. behavior evaluated final arrangement bit error ratio (BER)...

So-called stacking forces, of particular interest in the structural stability nucleic acids, have been studied a simple model system planar, non-hydrogen-bonding dye molecules (Methylene Blue). The dimerization process has by spectral analyses at different concentrations and temperatures D2O as well H2O. Computer-aided data-processing techniques used to obtain detailed information on process.Results show that high positive (hydrophobic) contribution 'unitary' entropy term due solute–solvent...

Abstract We report on experiments showing that the presence of some monohydric alcohols in solution medium perturbs kinetics bovine liver β‐galactosidase. The effect increases with increasing alcohol concentration and alkyl group size. To explain this we suggest a model considers existence functional nonfunctional enzyme conformations thermodynamical equilibrium. This equilibrium is dependent both

An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels different dimensions for purposes, is described. Preparation such as easy agarose gel preparation, polymerized are used to fractionate proteins or small DNA fragments using common electrophoretic tank. This was electrophoretically immuno-blot analyses, particularly in the study allergenic response Parietaria judaica pollen senescence, Southern-blot hybridizations polymorphisms.

The spectrometer of the ATLAS experiment has been designed to identify muon tracks with transverse momenta up 1 TeV/c. Its barrel section is made monitored drift tubes and resistive plate chambers (RPCs) arranged into 32 sectors, form two wheels surrounding interaction point. RPC subsystem provides level-1 trigger in it read out by a specific DAQ system. On-detector electronics pack data frames an event number assigned logic transmit them counting room optically. Data from each sector are...

Typically, TDAQ systems of High Energy Physics experiments, require time measurements, for example in synchronization or calibration activities. Usually, mixed-signal design is used the measurement circuits, but recent past research also studying all-digital design, that have many advantages with respect to previous approach. In this work, we present a full-digital TDC application, based on synthesizable DCO. The DCO technology-independent, it described by means hardware description language...

The construction and design process of a high-resolution time-interval measuring system implemented in SRAM-based FPGA device is discussed this paper. TDC can increase the precision on measurement by interpolating time within clock cycle. A two step phase interpolation has been performed, one based information delivered VIRTEX-5 DCM thus providing fine time, second level was carry lines delivering an iper measurement. We have designed built PCB hosting Virtex-5 Xilinx FPGA. In paper we show...

Pollen allergens are multivalent proteins that cross‐link IgE antibodies on mast or basophil cells, inducing secretion of biologic mediators, and resulting in various allergic symptoms. The IgE‐binding regions the Parietaria judaica ( Pj ) pollen major allergen rPar j 2 were investigated. Twenty‐nine single sera from ‐allergic subjects tested by Western blot against five recombinant peptides. At least four putative epitopes identified. analysis their diffusion suggested a heterogeneous...

Abstract Par j 2.0101, a major allergen of the Parietaria judaica pollen, was expressed in E. coli, purified to homogeneity and fully characterised both at structural functional level. The recombinant rPar 2.0101 protein showed an allergenic activity histamine release, skin prick tests capacity bind IgE, almost identical that native allergens from aqueous pollen extract. complete pattern S-S bridges determined by enzymatic digestion with endoproteinase Lys-C followed mass spectrometric...

High-speed analog-to-digital converters (ADCs) are key components in a huge variety of systems, including trigger and data acquisition (TDAQ) systems nuclear subnuclear physics experiments. Over the last decades, sample rate dynamic range high-speed ADCs underwent continuous growth, it required development suitable interface protocols, such as new JESD204B serial protocol. In this paper, we present an original JESD204B-compliant architecture designed, which is able to operate ADC remote...

Dense wavelength division multiplexing (DWDM) is a technology that assigns to the users of an optical network specific wavelengths or "colors" and mixes them in single strand fiber. Heterogeneous data transmitted simultaneously across lanes light, which are demuxed at receiving end delivered clients. Instead deploying more fibers increasing transfer rate, bandwidth link scalable by adding system. DWDM has been pioneered for applications long-haul telecom networks, requiring bulky,...