A5043909142- Marine Biology and Environmental Chemistry
- Genomics, phytochemicals, and oxidative stress
- Silk-based biomaterials and applications
- Echinoderm biology and ecology
- Lipid Membrane Structure and Behavior
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- Insect Resistance and Genetics
- Marine and coastal plant biology
- Insect and Arachnid Ecology and Behavior
- RNA Research and Splicing
- Food Allergy and Anaphylaxis Research
- Alzheimer's disease research and treatments
- Allergic Rhinitis and Sensitization
- Neurobiology and Insect Physiology Research
- Plant Reproductive Biology
- Contact Dermatitis and Allergies
- Protein Hydrolysis and Bioactive Peptides
- Invertebrate Immune Response Mechanisms
- Insect-Plant Interactions and Control
- Bacteriophages and microbial interactions
- Environmental Toxicology and Ecotoxicology
- Fungal Biology and Applications
- Supramolecular Self-Assembly in Materials
- Molecular Biology Techniques and Applications
Istituto di Farmacologia Traslazionale
2023
Institute for Biomedical Research and Innovation
2020-2022
Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy
2008-2019
National Research Council
2009
Instituto di Biofisica
1994-2007
Institute of Nanostructured Materials
2007
University of Palermo
1990-2007
United States Nuclear Regulatory Commission
1996
Oxidative stress and dysfunctional mitochondria are among the earliest events in AD, triggering neurodegeneration. The use of natural antioxidants could be a neuroprotective strategy for blocking cell death. Here, antioxidant action ferulic acid (FA) on different paths leading to degeneration recombinant beta-amyloid peptide (rAbeta42) treated cells was investigated. Further, improve its delivery, novel drug delivery system (DDS) used. Solid lipid nanoparticles (SLNs), empty or containing...
Ferulic acid (FA), a phenolic compound with significant antioxidant activity in Alzheimers disease, was entrapped into several solid lipid nanoparticles (SLN and NLC) by using the microemulsion technique. Stable SLN NLC formulations having mean size ranging between 94-140 nm high zeta potential were obtained. The sample obtained as matrix Compritol 888 ATO chosen for further characterization because, among these particles, showed Loading Capacity (LC%) best characteristics terms of size,...
Gene transfer represents an important advance in the treatment of both genetic and acquired diseases. In this article, suitability cationically modified solid-lipid nanoparticles (SLN) as a nonviral vector for gene delivery was investigated, order to obtain stable materials able condense RNA. Cationic SLN were produced by microemulsion using Compritol ATO 888 matrix lipid, Pluronic F68 tenside, dimethyldioctadecylammonium bromide (DDAB) cationic lipid. The resulting particles approximately...
Substance P (SP) is a neuropeptide that mediates many physiological as well inflammatory responses. Recently, SP has been implicated in the resolution of inflammation through induction M2 macrophages phenotype. The shift between M1-like and M2-like, allowing processes, also takes place by means hemeoxygenase-1 (HO-1). HO-1 induced response to oxidative stress stimuli modulates immune polarisation. induces expression human periodontal ligament (PDL), latter potentially plays role...
A distinctive feature of Alzheimer's disease is the deposition amyloid beta-protein (Abeta) in senile or diffuse plaques. The 42 residue beta-peptide (Abeta42) predominant form found In present work we report a high-yield expression and purification method production recombinant Abeta42. purified peptide shows characteristics similar to synthetic human peptide. Different size aggregates, either small oligomers larger were obtained upon dissolving Abeta42 under different conditions at pH 7.2...
We assessed the relationship between V, Cr, Mn, Hg, As, Cd, Sn, Sb and Pb concentrations in Mytilus galloprovincialis samples from coasts of Sicily expression metallothioneins. Toxic mineral elements assessment was carried out by A.A. Spectrometry ICP-MS. The metallothioneins performed q-PCR method. Low metals' levels were found mussel examined, comparison with what reported literature. highest mean values toxic Gela (Cr 0.178 ± 0.03 mg/Kg, Mn 4.325 0.012 As 3.706 0.009 Sn 0.148 0.014 0.004...
Parietaria pollen is one of the major cause pollinosis in southern Europe. Specific immunotherapy only treatment able to modify natural outcome disease restoring a normal immunity against allergens.We designed recombinant molecule (PjEDloop1) comprised genetic-engineered variants allergens (Par j 2/Par 1). Purity and chemical-physical properties derivative were analysed by RP-HPLC chromatography Photon Correlation Spectroscopy. Immunological activity was evaluated means Western blotting,...
We demonstrated that two mRNAs are synthesized during the vitellogenic period of oogenesis and code for cell surface proteins asymmetrically distributed in unfertilized egg Paracentrotus lividus. At fertilization, these RNAs rapidly localize cortical zone at animal pole egg. They then detected mesomeres macromeres, but not micromeres, thereafter found ectoderm vegetal plate, mesenchyme cells, or early intestine. disappear late gastrula. The by show same territorial location examined here,...
Abstract Two cDNA clones related to Paracentrotus lividus butanol‐extracted proteins, presumably belonging cell surface were isolated by a λgt11 expression library of ovary poly A + RNA. These clones, called bep 1 and bep4, 1,110 1,071 bp, respectively, belong multigene family. By sequencing analysis, special structural organization in the coding region is detected. single copy inserted between two regions different from each other but similar which constitute perfectly preserved domains...
Abstract: Parietaria pollen is one of the major causes allergic reaction in southern Europe, affecting about 30% all patients this area. Specific immunotherapy only treatment able to modify natural outcome disease by restoring a normal immunity against allergens. The preparation allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica allergen Par j 2, was investigated. 2 expressed large amount Escherichia coli and purified homogeneity. Its immunological...
The Keap1 protein is the master modulator of Nrf2 pathway; moreover, it hub such important processes as cancer, cell stress, inflammation, and chemio- radio-resistance. That why has become an intriguing pharmacological target. Many recent data show that interacts with HSP90 protein. In this study, we use ferulic acid (FA) antioxidant anti-inflammatory agent, able to relieve inflammatory response. It known treatment 100 μg FA can significantly decrease oxidative so turns be useful study...
Bep mRNAs, i.e., maternal messengers coding for cell surface proteins, are localized in the animal part of Paracertrotus lividus egg and embryos. Here we have examined involvement cytoskeleton asymmetric distribution bep3 mRNA. Moreover, order to understand whether how cis ‐and trans ‐acting factors necessary mRNA localization, looked vitro ‐specific interactions between proteins By northwestern assay identified a 54‐kDa protein that binds 3′UTR This also permits association with elements,...
We have isolated and characterized a cDNA clone corresponding to new member of bep (butanol, extracted, proteins) Paracentrotus lividus multigene family coding for cell surface proteins. The cDNA, called bep3, encodes 370 amino acid protein shares the same structural organization in region with other members gene already characterized. Expression this studied by Northern blot whole mount hybridization shows that bep3 messenger is transcribed during oogenesis utilized till gastrula stage,...
Bep mRNAs are localized at the animal pole of P. lividus eggs. In present communication secondary structures 3'UTRs bep1, bep3 and bep4 reported. The minimal lengths these regions required to bind 54-kDa protein, previously shown be involved in localization anchoring RNAs, is estimated. Microinjection 3'UTR into egg shows that this RNA fragment also able become one poles, as happens for entire RNA.