A5074931366- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Advanced Proteomics Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Hemoglobin structure and function
- Protein purification and stability
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Endoplasmic Reticulum Stress and Disease
- Polyamine Metabolism and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Hydrolysis and Bioactive Peptides
- Cultural Heritage Materials Analysis
- Chemical Synthesis and Analysis
- Nitric Oxide and Endothelin Effects
- Algal biology and biofuel production
- Heat shock proteins research
- Analytical Chemistry and Chromatography
University of Naples Federico II
2014-2024
Università degli Studi Suor Orsola Benincasa
2015-2024
Tunis El Manar University
2013
Institut Pasteur de Tunis
2013
University of the Basque Country
2013
Consejo Superior de Investigaciones Científicas
2013
University of Molise
2013
Istituto Neurologico Mediterraneo
2002-2006
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2005
Ceinge Biotecnologie Avanzate (Italy)
1996-2004
A considerable fraction of life develops in the sea at temperatures lower than 15°C. Little is known about adaptive features selected under those conditions. We present analysis genome sequence fast growing Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. find that it copes with increased solubility oxygen low temperature by multiplying dioxygen scavenging while deleting whole pathways producing reactive species. Dioxygen-consuming lipid desaturases achieve both protection against...
Abstract Biofilm is the most successful and widely distributed form of life on earth, it not simply structured collections cells attached to surfaces but a dynamic complex biological system able respond environmental changes. The biofilm characteristics make unique central microbial evolution adaptation. ability establish biofilms key trait for microorganisms growing in extreme environments like temperature, high radiation, acidic or alkaline pH values, heavy metal pollution, salinity. In...
By a combination of 1D and 2D 1 H‐ 13 C‐NMR, FAB‐MS, chemical enzymatic reactions carried out at the milligram level, it has been demonstrated that syringomycin E, major phytotoxic antibiotic produced by Pseudomonas syringae pv. , is new lipodepsipeptide. Its amino acid sequence Ser‐Ser‐Dab‐Dab‐Arg‐Phe‐Dhb‐4(Cl)Thr‐3(OH)Asp with β‐carboxy group C‐terminal residue closing macrocyclic ring on OH N‐terminal Ser, which in turn N ‐acylated 3‐hydroxydodecanoic acid. Syringomycins A G, two other...
Hypertension affects nearly 20% of the population in Western countries and strongly increases risk for cardiovascular diseases. In pathogenesis hypertension, vasoactive peptide renin-angiotensin system, angiotensin II its G protein–coupled receptors (GPCRs), play a crucial role by eliciting reactive oxygen species (ROS) mediating vessel contractility. Here we show that mice lacking GPCR-activated phosphoinositide 3-kinase (PI3K)γ are protected from hypertension is induced administration...
Human interleukin-6 or B-cell stimulatory factor-2 is a cytokine involved in acute phase and immune response. Cloning of cDNA for human the pT7.7 expression plasmid under control bacteriophage T7 RNA polymerase promoter system allows rapid production Escherichia coli. Upon cell induction with isopropyl thiogalactopyranoside, recombinant overexpressed forms insoluble inclusion bodies. Solubilization protein 6 M guanidine hydrochloride refolding presence reduction/oxidation results...
ABSTRACT A new extracellular protease (PoSl; Pleurotus ostreatus subtilisin-like protease) from P. culture broth has been purified and characterized. PoSl is a monomeric glycoprotein with molecular mass of 75 kDa, pI 4.5, an optimum pH in the alkaline range. The inhibitory profile indicates that serine protease. N-terminal three tryptic peptide sequences have determined. homology one internal conserved sequence around Asp residue catalytic triad subtilase family suggests This hypothesis...
The primary structure of some new lipodepsipeptides named syringopeptins, produced by plant pathogenic strains Pseudopmonas syringae pv. has been determined a combination chemical methods, 1 H and 13 C NMR spectroscopy FAB mass spectrometry. Two syringomycin‐producing afforded 3‐hydroxydecanoyl‐Dhb‐Pro‐Val‐Val‐Ala‐Ala‐Val‐Val‐Dhb‐Ala‐Val‐Ala‐Ala‐Dhb‐aThr‐Ser‐Ala‐Dhb‐Ala‐Dab‐Dab‐Tyr, with Tyr acylating Thr to form macrolactone ring, smaller amounts the 3‐hydroxydodecanoyl homologue. Evidence...
Proteomic strategies are herein proved to be a complementary approach the well established amino acid composition analysis for characterization of aging and deterioration phenomena occurring proteinaceous materials in works-of-art. Amino analyses on several samples demonstrated that proteins frescoes from Camposanto Monumentale Pisa deteriorated as revealed by decrease Met, Lys, Tyr content presence all malonic result Ser, Phe, Cys oxidation. identified deamidation at Asn Gln further major...
The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacterium isolated from subzero Arctic marine sediments, provides model study life in cold environments. We report here identification detailed molecular primary secondary structures capsular polysaccharide...
Staphylococcus epidermidis is a harmless human skin colonizer responsible for approximately 20% of orthopaedic device-related infections due to its capability form biofilm. Nowadays there an interest in the development anti-biofilm molecules. Marine bacteria represent still underexploited source biodiversity able synthesize broad range bioactive compounds, including Previous results have demonstrated that culture supernatant Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125...
In order to exploit the rich reservoir of marine cold-adapted bacteria as a source bioactive metabolites, ethyl acetate crude extracts thirteen polar were tested for their antiproliferative activity on A549 lung epithelial cancer cells. The extract from Pseudoalteromonas haloplanktis TAC125 was most active in inhibiting cell proliferation. Extensive bioassay-guided purification and mass spectrometric characterization allowed identification 4-hydroxybenzoic acid (4-HBA) molecule responsible...
Alzheimer's disease (AD) has been recently associated with vascular risk factors. β-amyloid peptides (AβP), the main component of senile plaques typical AD, circulate in soluble globular form bloodstream. Interestingly, AβP is able to induce endothelial dysfunction, and this effect may represent link between neuronal pathophysiological factors involved AD. We aimed clarify molecular mechanisms underlying AβP-induced toxicity. Using several methodological approaches, we have observed that...
Monitoring food quality is a critical task for analytical chemistry and an important way to preserve human health. Fish valuable source of highly digestible proteins contains large amounts polyunsaturated fatty acids fat-soluble vitamins. Since the world's wild fish stocks are limited, farmed nowadays proposed as alternative consumers. It now emerging that muscle protein content assuming great importance from aquaculture perspective. Many data have been collected on physiology biochemistry...
The gene coding for aspartate aminotransferase (EC 2.6.1.1) has been cloned from the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus strain MT4. Partial sequence data obtained directly purified protein and two cyanogen‐bromide‐generated peptides confirm primary structure of inferred nucleotide its gene. A comparison enzyme with other aminotransferases revealed an interesting similarity tyrosine rat liver 2.6.1.5) allowed some tentative assignments residues implied in...
Aspartate aminotransferase from the archaebacterium Sulfolobus solfataricus, a thermoacidophilic organism isolated an acidic hot spring (optimal growth conditions: 87 OC, pH 3.5) was purified to homogeneity, The enzyme is dimer (Mr subunit = 53,000) showing microheterogeneity when submitted chromatofocusing and/or isoelectric focusing analysis (two main bands having PI 6.8 and 6.3 were observed).The N-terminal sequence (22 residues) does not show any homology with stretch of known aspartate...