A5019815285- Cultural Heritage Materials Analysis
- Enzyme Structure and Function
- Conservation Techniques and Studies
- Protein Structure and Dynamics
- Amino Acid Enzymes and Metabolism
- Building materials and conservation
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Biosensors and Analytical Detection
- Enzyme-mediated dye degradation
- Enzyme Catalysis and Immobilization
- Microbial bioremediation and biosurfactants
- Identification and Quantification in Food
- Biochemical and Molecular Research
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Collagen: Extraction and Characterization
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Bone Tissue Engineering Materials
- Cardiomyopathy and Myosin Studies
- Ubiquitin and proteasome pathways
- bioluminescence and chemiluminescence research
- Cancer, Hypoxia, and Metabolism
University of Naples Federico II
2015-2024
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2015-2018
Institute of Organic Chemistry
2010
European Molecular Biology Laboratory
2000
University of Basel
1998
Sapienza University of Rome
1997
Cardiff University
1995
Twenty-four Actinobacteria strains, isolated from Arundo donax, Eucalyptus camaldulensis and Populus nigra biomasses during natural biodegradation with potential enzymatic activities specific for the degradation of lignocellulosic materials, were identified by a polyphasic approach. All strains belonged to Streptomyces (S.) genus in particular, most representative species was S. argenteolus including 50% strains; while 8 as members flavogriseus (synonym flavovirens) fimicarius (synonyms...
Laccases are oxidative enzymes linked to biological degradation of lignin. The aim this work was evaluate the effect inducers and different concentrations nitrogen on production level total laccase activity pattern isoforms, produced in solid state fermentation sugarcane bagasse by a selected strain Pleurotus ostreatus. addition yeast extract 5 g/L, copper sulfate 150 μM ferulic acid 2 mM provided highest enzymatic (167 U/g) zymograms indicated presence six isoforms (POXA1b, POXA3, POXC...
Proteomic strategies are herein proved to be a complementary approach the well established amino acid composition analysis for characterization of aging and deterioration phenomena occurring proteinaceous materials in works-of-art. Amino analyses on several samples demonstrated that proteins frescoes from Camposanto Monumentale Pisa deteriorated as revealed by decrease Met, Lys, Tyr content presence all malonic result Ser, Phe, Cys oxidation. identified deamidation at Asn Gln further major...
Gas chromatography/mass spectrometry, proteomic and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS) analyses of residue on a stone flake from 49,000 year-old layer Sibudu (South Africa) indicate mixture ochre casein milk, likely obtained by killing lactating wild bovid. Ochre powder production use are documented in Middle Stone Age South African sites but until now there has been no evidence the milk as binder. Our show that this ochre-based was neither...
Abstract ICOSL/ICOS are costimulatory molecules pertaining to immune checkpoints; their binding transduces signals having anti-tumor activity. Osteopontin (OPN) is here identified as a ligand for ICOSL. OPN binds different domain from that used by ICOS, and the induces conformational change in OPN, exposing domains relevant its functions. Here we show vitro, ICOSL triggering cell migration, while inhibiting anchorage-independent growth. The mouse 4T1 breast cancer model confirms these data....
Abstract Background The use of lignocellulosic materials for second generation ethanol production would give several advantages such as minimizing the conflict between land food and fuel production, providing less expensive raw than conventional agricultural feedstock, allowing lower greenhouse gas emissions those first ethanol. However, cellulosic biofuels are not produced at a competitive level yet, mainly because high costs cellulolytic enzymes. Therefore, this study was aimed discovering...
Cellulases and xylanases are the key enzymes involved in conversion of lignocelluloses into fermentable sugars. Western Ghat region (India) has been recognized as an active hot spot for isolation new microorganisms. The aim this work was to isolate microorganisms producing cellulases be applied brewer's spent grain saccharification.93 were isolated from screened production cellulase xylanase activities. Fourteen cellulolytic seven xylanolytic further liquid culture. Particular attention...
Ninety bacteria isolated from raw composting materials were screened for their cellulolytic activity on solid medium containing carboxymethylcellulose. The producing the highest levels identified by 16S rRNA sequencing as Bacillus licheniformis strain 1, subtilis subsp. B7B, spizizenii 6, and amyloliquefaciens B31C. Cellulase production most productive B. B31C was optimized in liquid culture varying carbon source. Comparison of growth curves at temperatures 28 to 47 °C indicated its...
Two fungal strains, Aspergillus terreus MUT 271 and Trichoderma harzianum 290, isolated from a Mediterranean marine site chronically pervaded by oil spills, can use crude as sole carbon source. Herein, these strains were investigated producers of biosurfactants, apt to solubilize organic molecules preliminary step metabolize them. Both fungi secreted low molecular weight proteins identified cerato-platanins, small, conserved, hydrophobic proteins, included among the surface-active proteins....
The gene encoding aspartate aminotransferase from the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC 125 was cloned, sequenced and overexpressed in Escherichia coli . recombinant protein ( Ph AspAT) characterized both at structural functional level comparison with E. enzyme Ec AspAT), which is most closely related (52% sequence identity) bacterial counterpart. AspAT rapidly inactivated 50 °C (half‐life = 6.8 min), whereas this temperature stable for least 3 h. optimal activity ≈...
RATIONALE The aim of this study was to demonstrate, and characterize by high‐resolution mass spectrometry that it is possible preferentially induce covalent cross‐links in peptides using high‐energy femtosecond ultraviolet (UV) laser pulses. cross‐link readily formed only when aromatic amino acids are present the peptide sequence. METHODS Three peptides, xenopsin, angiotensin I, interleukin, individually or combination, were exposed UV pulses, either alone presence spin trapping molecules,...
A novel method for the analysis of proteinaceous materials present on painted surfaces was developed by taking advantage adhesive ability some fungal proteins which can form a stable and homogeneous layer flexible transparency sheets able to capture trypsin in fully active form. We demonstrated that bioactive were efficiently digest proteins, as such, tests historical samples, releasing peptides allow an easy confident identification binders standard bottom-up proteomic approach. By this...
Animal glues are widely used in restoration as adhesives, binders, and consolidants for organic inorganic materials. Their variable performances intrinsically linked to the adhesive properties of collagen, which determine chemical, physical, mechanical glue. We have molecularly characterized protein components a range homemade commercial using mass spectrometry techniques. A shotgun proteomic analysis provided animal origin, even when blended, allowed us distinguish between hide bone glue on...
A combination of hydrogen/deuterium (H/D) exchange and limited proteolysis experiments coupled to mass spectrometry analysis was used depict the conformation in solution HAMLET, folding variant human alpha-lactalbumin, complexed oleic acid, that induces apoptosis tumor immature cells. Although near- far-UV CD fluorescence spectroscopy were not able discriminate between HAMLET apo-alpha-lactalbumin, H/D clearly showed they correspond two distinct conformational states, with incorporating a...
Catechol 2,3-dioxygenase (C2,3O) from <i>Pseudomonas stutzeri</i> OX1, which is able to grow on various aromatic substrates as the sole source of carbon and energy, has been expressed in <i>Escherichia coli</i>, purified, characterized, found be very similar other dioxygenases <i>Pseudomonas</i> species. Interestingly, activity protein shows a rather unusual pH dependence when assayed catechol. A model catalytic mechanism was developed that reproduce behavior function pH. The includes...
A chemical investigation of the organic paint binders Giant Buddhas Bāmiyān was performed using an analytical approach based on mass spectrometry, combining traditional gas chromatography/mass spectrometry protocols with advanced proteomics methodologies. The research carried out a selection rescued fragments. data revealed use egg proteins as original layers, in accordance this proteinaceous medium antiquity, spanning from Mediterranean basin to Far East, and already Bronze Age. Egg tempera...
Titin is an exceptionally large protein (M.Wt. approximately 3 MDa) that spans half the sarcomere in muscle, from Z-disk to M-line. In Z-disk, it interacts with alpha-actinin homodimers are a principal component of Z-filaments linking actin filaments. The interaction between titin and involves repeating 45 amino acid sequences (Z-repeats) near N-terminus C-lobe C-terminal calmodulin-like domain alpha-actinin. conformation Z-repeat 7 (ZR7) when complexed 73-amino portion (EF34) was studied by...
Identification of proteins in paintings and polychrome objects is a challenge, which requires the development tailored analytical approaches. In present study, targeted proteomics approach was developed for discriminating among three most common proteinaceous materials used as paint binders, i.e. milk, egg, animal glue. this study specific database peptides created based on tandem MS analyses tryptic digests several samples collected from variety art different ages conservation conditions....
Pectinolytic enzymes play an important role in the processing of lignocellulosic materials because their ability to improve access cellulases substrate by removing pectins.The strain Paenibacillus xylanolyticus 2-6L3 was isolated from mature compost obtained agroindustrial wastes, and enzyme pectate lyase P. 2-6L3, named PaenxylPel, partially purified subjected structural functional characterisation.The exhibited optimum temperature between 60 70 °C optimal pH value 9.0 for its pectinase...