A5074690135- Cultural Heritage Materials Analysis
- Mass Spectrometry Techniques and Applications
- Conservation Techniques and Studies
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Building materials and conservation
- Identification and Quantification in Food
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- Fermentation and Sensory Analysis
- Advanced Combustion Engine Technologies
- Heme Oxygenase-1 and Carbon Monoxide
- Oral microbiology and periodontitis research
- Carbohydrate Chemistry and Synthesis
- Microbial Community Ecology and Physiology
- Parasitic Infections and Diagnostics
- Metabolomics and Mass Spectrometry Studies
- Tea Polyphenols and Effects
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Mitochondrial Function and Pathology
- Peptidase Inhibition and Analysis
- Proteoglycans and glycosaminoglycans research
- Dental materials and restorations
University of Naples Federico II
2016-2025
Università degli Studi Suor Orsola Benincasa
2020-2024
Federico II University Hospital
2011-2020
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2009-2013
Boston University
2010-2013
Research Center Borstel - Leibniz Lung Center
2009
Sigma Tau (Italy)
2006
ABSTRACT Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most gram-negative bacteria, and its structure biosynthetic pathway are well known. Nevertheless, mechanisms transport assembly this molecule at cell surface poorly understood. The inner (IM) protein MsbA responsible for flipping LPS across IM. Additional components machinery downstream have been identified, including OM complex LptD/LptE (formerly Imp/RlpB), periplasmic LptA protein, IM-associated...
The prototypic long pentraxin PTX3 is a unique fluid-phase pattern recognition receptor that plays nonredundant role in innate immunity and female fertility. C-terminal domain required for C1q complement activation contains single N-glycosylation site on Asn 220. In the present study, we characterized structure of human glycosidic moiety investigated its relevance interaction classical pathway. By specific endo exoglycosidases digestion direct mass spectrometric analysis, found both...
Arbuscular mycorrhizal fungi (AMF) are obligate plant biotrophs that may contain endobacteria in their cytoplasm. Genome sequencing of Candidatus Glomeribacter gigasporarum revealed a reduced genome and dependence on the fungal host. RNA-seq analysis AMF Gigaspora margarita presence absence endobacterium indicated have an important role pre-symbiotic phase by enhancing bioenergetic capacity. To improve understanding fungal-endobacterial interactions, iTRAQ (isobaric tags for relative...
ABSTRACT Coccidia are protozoan parasites that cause significant human disease and of major agricultural importance. Cryptosporidium spp. diarrhea in humans animals, while Toxoplasma causes disseminated infections fetuses untreated AIDS patients. Eimeria is a pathogen commercial chickens. Oocysts, which the infectious form one two forms (the other tissue cysts undercooked meat), have multilayered wall. Recently we showed inner layer oocyst walls porous scaffold fibers β-1,3-glucan, also...
Glioblastoma Multiforme (GBM) is the most common and aggressive human brain tumor, associated with very poor survival despite surgery, radiotherapy chemotherapy.The epidermal growth factor receptor (EGFR) platelet-derived β (PDGFRβ) are hallmarks in GBM driving roles tumor progression. In approximately half of tumors amplified EGFR, EGFRvIII truncated extracellular mutant detected. does not bind ligands, highly oncogenic its expression confers resistance to EGFR tyrosine kinase inhibitors...
Commercial uses of bioactive peptides require low cost, effective methods for their production. We developed a new carrier protein high yield production recombinant in Escherichia coli very well suited the toxic like antimicrobial peptides. GKY20, short peptide derived from C-terminus human thrombin, was fused to Onconase, small ribonuclease (104 amino acids), which efficiently drove into inclusion bodies with expression levels (about 200-250 mg/L). After purification fusion by immobilized...
The exploitation of easily accessible and nontoxic natural catechol compounds for surface functionalization coating is attracting growing interest biomedical applications. We report herein the deposition on different substrates chemically stable thin films by autoxidation 1 mM caffeic acid (CA) solutions at pH 9 in presence equimolar amounts hexamethylenediamine (HMDA). UV–visible, mass spectrometric, solid state 13C 15N NMR analysis indicated covalent incorporation amine during CA...
The infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or duodenalis) is the cyst. cyst wall contains fibrils a unique β-1,3-linked N-acetylgalactosamine (GalNAc) homopolymer at least three proteins (CWPs) composed Leu-rich repeats (CWPLRR) C-terminal conserved Cys-rich region (CWPCRR). Our goals were to dissect structure determine how it disrupted during excystation. intact thin (∼400 nm), easily fractured by sonication, impermeable small molecules. Curled...
The use of indwelling medical devices is associated with a significant risk infections by Staphylococcus aureus (S. aureus) which possesses variety virulence factors including many toxins and the ability to invade eukaryotic cells or form biofilm on biotic abiotic surfaces. above described are often related proteins exposed bacterial surface. Blocking S. colonization may reduce incidence invasive infectious diseases. Previously reports evaluated anti-infective properties serratiopeptidase...
Chemical compounds within tea (Camellia sinensis) are characterized by an extensive heterogeneity; some of them crucial for their protective and defensive role in plants, closely connected to the benefits that consumption can provide. This paper is mainly focused on characterization polyphenols (secondary metabolites generally involved defense against ultraviolet radiation aggression pathogens) metals, extracted from nine Chinese samples, integrating different mass spectrometry...
Summary As other arbuscular mycorrhizal fungi, Gigaspora margarita contains unculturable endobacteria in its cytoplasm. A cured fungal line has been obtained and showed it was capable of establishing a successful colonization. However, previous OMICs physiological analyses have demonstrated that the fungus is impaired some functions during pre‐symbiotic phase, leading to lower respiration activity, ATP, antioxidant production. Here, by combining deep dual‐mRNA sequencing proteomics applied...
Animal glues are widely used in restoration as adhesives, binders, and consolidants for organic inorganic materials. Their variable performances intrinsically linked to the adhesive properties of collagen, which determine chemical, physical, mechanical glue. We have molecularly characterized protein components a range homemade commercial using mass spectrometry techniques. A shotgun proteomic analysis provided animal origin, even when blended, allowed us distinguish between hide bone glue on...
Dramatic changes in glycan biosynthesis during oncogenic transformation result the emergence of marker glycans on cell surface. We analysed N-linked L1CAM from different stages melanoma progression, using high-performance liquid chromatography combined with exoglycosidase sequencing, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and lectin probes. oligosaccharides are heavily sialylated, mainly digalactosylated, biantennary complex-type structures galactose...
Examining the features distinguishing organic carbon from soot is crucial for understanding source, effect on environment and their respective role in aerosol chemistry formation. Beside to obvious PAH picking-out low-mass mode (C number < 40), challenging identification of PAHs high-mass > 40) carbon, separated by particulate matter extraction young mature thermophoretically sampled premixed flames, was done laser-desorption-time-of-flight mass spectrometry, exploiting laser power increase....
Ovarian cancer is the fifth leading cause of cancer-related deaths in women. Standard treatment consists tumor debulking surgery followed by platinum and paclitaxel chemotherapy; yet, despite initial response, about 70-75% patients develop resistance to chemotherapy. Gold compounds represent a family very promising anticancer drugs. Among them, we previously investigated cytotoxic pro-apoptotic properties Au(NHC) Au(NHC)2PF6, i.e., monocarbene gold(I) complex corresponding bis(carbene)...