A5029900874- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Lipid Membrane Structure and Behavior
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Endoplasmic Reticulum Stress and Disease
- Academic Publishing and Open Access
- Bacterial biofilms and quorum sensing
- Ethics in Clinical Research
- Fungal and yeast genetics research
- scientometrics and bibliometrics research
- Public Health Policies and Education
- Cellular transport and secretion
- Zoonotic diseases and public health
- CRISPR and Genetic Engineering
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
Princeton University
2015-2024
Merck & Co., Inc., Rahway, NJ, USA (United States)
2019
Brown University
2016
Google (United States)
2015
Harvard University
1977-2008
Howard Hughes Medical Institute
2007
Washington University in St. Louis
2005
University of California, San Francisco
1996
University of Washington
1993
University of Illinois Urbana-Champaign
1992
The outer membranes (OMs) of Gram-negative bacteria have an asymmetric lipid distribution with lipopolysaccharides at the leaflet and phospholipids (PLs) inner leaflet. This arrangement is essential for barrier function OM viability most bacteria. Cells assembly defects or cells exposed to harsh chemical treatments accumulate PLs in this disrupts lipopolysaccharide organization increases sensitivity small toxic molecules. We identified ABC transport system Escherichia coli predicted import...
Integral β-barrel proteins (OMPs) are a major class of outer membrane in Gram-negative bacteria. In Escherichia coli , these synthesized the cytoplasm, translocated across inner via Sec machinery, and assembled through an unknown mechanism that requires YaeT complex periplasmic chaperones SurA, DegP, Skp. Here, we have established relationship between three providing insight into OMP biogenesis using depletion analysis. Depletion SurA alone results marked decrease density, while loss DegP...
Bacterial adhesion is an important initial step in biofilm formation, which may cause problems medical, environmental, and industrial settings. In spite of obvious phenotypic differences between attached planktonic cells, knowledge about the genetic basis for these how adhesion-induced changes are mediated limited. The Cpx two-component signal transduction pathway responds specifically to stress caused by disturbances cell envelope activates genes encoding periplasmic protein folding...
Integral beta-barrel proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria. The machine that assembles these contains an integral membrane protein, called YaeT Escherichia coli, which has one or more polypeptide transport-associated (POTRA) domains. crystal structure a periplasmic fragment reveals POTRA domain fold suggests model for how domains can bind different peptide sequences, as required handles numerous protein precursors. Analysis...
The outer membrane of most Gram-negative bacteria is made up LPS, and in nearly all that contain LPS it essential for the life organism. lipid portion this molecule, A, also known as endotoxin, a potent activator innate immune response. More than 50 genes are required to synthesize assemble at cell surface. Enormous progress has been elucidating structure biosynthesis but until recently cellular components its transport from site synthesis inner final location surface remained elusive. Here...
The kinetic parameters for the maltose transport system in Escherichia coli K12 were determined with and maltotriose as substrates. exhibits an apparent Km of 1 μM 2 maltotriose. V entry was 2.0 1.1 nmol substrate/min per 108 cells. Mutations lamB, structural gene receptor protein phage λ, increased by a factor 100–500 without influencing maximal rate transport. Maltotriose is no longer transported these lamB mutants. maltose-binding protein, essential component system, found to exhibit...
Significance The spread of multidrug-resistant infections demands antibiotic development. Although new antibiotics targeting gram-positive bacteria have been developed, it has more than 50 y since a class gram-negative approved for clinical use. strong outer membrane permeability barrier makes difficult compounds to reach intracellular targets, contributing greatly the lack this microbes. Here, we describe compound, MRL-494, that inhibits assembly proteins into by an essential member...
DegP is a heat-shock inducible periplasmic protease in Escherichia coli. Unlike the cytoplasmic heat shock proteins, not transcriptionally regulated by classical regulon coordinated sigma 32. Rather, degP gene an alternate factor, E. Previous studies have demonstrated signal transduction pathway that monitors amount of outer-membrane proteins bacterial envelope and modulates levels response to this extracytoplasmic parameter. To analyze transcriptional regulation degP, we examined mutations...
Recent advances in the study of bacterial membranes have led to identification a multicomponent YaeT complex outer membrane (OM) Gram-negative bacteria that is involved targeting and folding beta-barrel proteins (OMPs). In Escherichia coli, this consists an essential OMP, YaeT, three OM lipoproteins, YfgL, NlpB YfiO. YfiO only lipoprotein component complex. We show required for proper assembly and/or OMPs but not lipopolysaccharides (LPS). Depletion causes similar phenotypes as does...