A5000795362- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Organometallic Complex Synthesis and Catalysis
- Asymmetric Hydrogenation and Catalysis
- Escherichia coli research studies
- Synthetic Organic Chemistry Methods
- Drug Transport and Resistance Mechanisms
- Protein Structure and Dynamics
- Enzyme Structure and Function
- HIV/AIDS drug development and treatment
- X-ray Diffraction in Crystallography
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Synthesis and characterization of novel inorganic/organometallic compounds
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Crystallization and Solubility Studies
- Hydrogen Storage and Materials
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune Response and Inflammation
- Carbon dioxide utilization in catalysis
- Metal complexes synthesis and properties
- Endoplasmic Reticulum Stress and Disease
The Ohio State University
2015-2024
Instituto Nacional de Pediatria
2012
Princeton University
2001-2011
Universidad de Navarra
2003
Weizmann Institute of Science
2003
Consejo Superior de Investigaciones Científicas
1996-2003
Centro de Investigaciones Biológicas Margarita Salas
2003
University of Colorado Health
2003
Universidad de Zaragoza
1991-2001
Washington University in St. Louis
1997-2001
Building the cell wall is flipping difficult The of bacteria constructed from a polysaccharide called peptidoglycan (PG). It forms matrix that surrounds cells and essential for integrity cytoplasmic membrane. Many our most successful antibiotics target PG synthesis. synthetic pathway involves assembly sugar building blocks on lipid carrier at inner face reactions produce this so-called II precursor enzymes catalyze them have been known decades. However, identity flippase enzyme “flips” in...
The outer membrane (OM) of most Gram-negative bacteria contains lipopolysaccharide (LPS) in the leaflet. LPS, or endotoxin, is a molecule important biological activities. In host, LPS elicits potent immune response, while bacterium, it plays crucial role by establishing barrier to limit entry hydrophobic molecules. Before assembled at OM, must be synthesized inner (IM) and transported across aqueous periplasmic compartment. Much known about biosynthesis but, until recently, little was its...
Peptidoglycan is a cell-wall glycopeptide polymer that protects bacteria from osmotic lysis. Whereas in Gram-positive it also serves as scaffold for many virulence factors, Gram-negative bacteria, peptidoglycan an anchor the outer membrane. For years, we have known enzymes required biosynthesis of peptidoglycan; what was missing flippase translocates lipid-anchored precursors across cytoplasmic membrane before their polymerization into mature peptidoglycan. Using reductionist bioinformatics...
Lipopolysaccharide (LPS) is the major glycolipid that present in outer membranes (OMs) of most Gram-negative bacteria. LPS molecules are assembled with divalent metal cations leaflet OM to form an impervious layer prevents toxic compounds from entering cell. For bacteria, essential for growth. In Escherichia coli, eight proteins have been identified function proper assembly following its biosynthesis. This process involves release inner membrane (IM), transport across periplasm, and...
Cell size varies greatly among different types of cells, but the range in that a specific cell type can reach is limited. A long-standing question biology how cells control their size. Escherichia coli adjusts and growth rate according to availability nutrients so it grows larger faster nutrient-rich media than nutrient-poor media. Here, we describe how, using classical genetics, have isolated remarkably small E. mutant has undergone 70% reduction volume with respect wild type. This lacks...
Abstract Little is known on how β‐barrel proteins are assembled in the outer membrane (OM) of Gram‐negative bacteria. SurA has been proposed to be primary chaperone escorting bulk mass OM across periplasm. However, impact deletion global proteome not determined, limiting therefore our understanding function SurA. By using a differential proteomics approach based 2‐D LC‐MS n , we compared relative abundance 64 proteins, including 23 wild‐type and surA strains. Unexpectedly, found that loss...
Gram-negative bacteria are impervious to many drugs and environmental stresses because they possess an outer membrane (OM) containing lipopolysaccharide (LPS). LPS is biosynthesized at the cytoplasmic (inner) transported OM by unknown mechanism involving transport proteins, LptA–G. These proteins have been proposed form a bridge between two membranes; however, it not known how this assembled prevent mistargeting of LPS. We use in vivo photo-cross-linking reveal specific protein–protein...
Most Gram-negative bacteria contain lipopolysaccharide (LPS), a glucosamine-based phospholipid, in the outer leaflet of membrane (OM). LPS is unique to bacterial OM and, most cases, essential for cell viability. Transport from its site synthesis surface requires eight proteins, MsbA and LptABCDEFG. Although key players have been identified, mechanism transport assembly not clear. The stable LptD/E complex present at functions final stages assembly. Here, we identified mutant allele lptE6,...
Significance The outer membrane of Gram-negative bacteria provides a barrier that allows these organisms to live in variety harsh environments. Here, we study the machine responsible for insertion integral β-barrel proteins into this membrane. How barrel assembly (Bam) functions is unknown because it has not been possible characterize substrates process folding. We slowed substrate and observe partially folded state still bound Bam complex. These studies define how where folding occurs roles...
The bacterial cell envelope is the first line of defense and point contact with environment other organisms. Envelope biogenesis therefore crucial for survival physiology bacteria often targeted by antimicrobials. Gram-negative have a multilayered delimited an inner outer membrane (IM OM, respectively). OM barrier against many antimicrobials because its asymmetric lipid structure, phospholipids composing leaflet lipopolysaccharides (LPS) leaflet. Since synthesis occurs at IM, LPS are...
Abstract In Saccharomyces cerevisiae , the transcriptional regulation of most glycolytic genes has been extensively studied. By contrast, little is known about control three glucose‐phosphorylating enzymes, although this catalytic reaction an important role in cell metabolism. paper, we describe HXK1, HXK2 and GLK1 hope revealing differences steady‐state levels mRNA associated with a particular carbon source used culture medium. Our results provide evidence supporting differential expression...
Small molecules that affect specific protein functions can be valuable tools for dissecting complex cellular processes. Peptidoglycan synthesis and degradation is a process in bacteria involves multiple enzymes under strict temporal spatial regulation. We used set of small inhibit the transglycosylation step peptidoglycan to discover genes help regulate this process. identified gene responsible susceptibility Escherichia coli cells killing by glycolipid derivatives vancomycin, thus...