A5082427892- Gut microbiota and health
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Genomics, phytochemicals, and oxidative stress
- Antioxidant Activity and Oxidative Stress
- Biochemical and Structural Characterization
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- Pneumonia and Respiratory Infections
- Autophagy in Disease and Therapy
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolites in Food Biotechnology
- Streptococcal Infections and Treatments
- Plant Molecular Biology Research
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Microbial Natural Products and Biosynthesis
Nanyang Technological University
2020-2025
Beijing Normal University
2024-2025
Jiangsu University of Science and Technology
2024
China-Japan Friendship Hospital
2021-2024
University of Electronic Science and Technology of China
2024
University of California, Irvine
2024
Yale University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Sichuan Agricultural University
2018-2023
Agency for Science, Technology and Research
2017-2021
Penicillin-binding proteins (PBPs) are involved in the synthesis and remodeling of bacterial peptidoglycan (PG). Staphylococcus aureus expresses four PBPs. Genetic studies S. have implicated PBP4 formation highly cross-linked PG, but biochemical not reached a consensus on its primary enzymatic activity. Using synthetic Lipid II, we show here that preferentially acts as transpeptidase (TP) vitro. Moreover, it is PBP primarily responsible for incorporating exogenous d-amino acids into cellular...
The commensal fungus Candida albicans often causes life-threatening infections in patients who are immunocompromised with high mortality. A prominent but poorly understood risk factor for the C. commensal‒pathogen transition is use of broad-spectrum antibiotics. Here, we report that β-lactam antibiotics cause bacteria to release significant quantities peptidoglycan fragments potently induce invasive hyphal growth albicans. We identify several active subunits, including tracheal cytotoxin, a...
The gut microbiota, often viewed as a "digestive organ," can influence the development of obesity and related metabolic disorders. Diet is significantly important in shaping structure modulating function microbiota. Apigenin (Api) widely exists fruits vegetables naturally occurring flavonoid has anti-obesogenic, anti-inflammatory, anti-carcinogenic properties. Its low bioavailability means it enough time to interact with intestine thus becomes potential substrate for intestine; thus,...
Lysobactin, also known as katanosin B, is a potent antibiotic with in vivo efficacy against Staphylococcus aureus and Streptococcus pneumoniae. It was previously shown to inhibit peptidoglycan (PG) biosynthesis, but its molecular mechanism of action has not been established. Using enzyme inhibition assays, we show that lysobactin forms 1:1 complexes Lipid I, II, II(A)(WTA), substrates the PG wall teichoic acid (WTA) biosynthetic pathways. Therefore, lysobactin, like ramoplanin teixobactin,...
The carotenoid isomerase gene (BoaCRTISO) of Chinese kale was targeted and edited using the CRISPR/Cas9 system in present study. results showed a high mutation rate (81.25%), 13 crtiso mutants were obtained. Only two types mutations, insertions replacements, found. Both total individual chlorophyll concentrations biallelic homozygous reduced, levels declined by 11.89-36.33%. color changed from green to yellow, likely reflecting reduction color-masking effect on carotenoids. expression most...
PGN_MS2: an in silico PGN library for automated LC-MS/MS spectral deconvolution of bacterial peptidoglycan fragments (PGNs).
Peptidoglycan (PG), an essential exoskeletal polymer in bacteria, is a well-known antibiotic target. PG polymerization requires the action of bacterial transglycosylases (TGases), which couple incoming glycosyl acceptor to donor. Interfering with TGase activity can interrupt assembly. Existing inhibitors like moenomycin and Lipid II analogues always occupy active sites; other strategies interfere proper elongation have not been widely exploited. Inspired by natural 1,6-anhydro-MurNAc termini...
Methicillin-resistant Staphylococcus aureus (MRSA) infections are a global public health problem. MRSA strains have acquired non-native penicillin-binding protein called PBP2a that cross-links peptidoglycan when the native S. PBPs inhibited by β-lactams. It has been proposed can use cell wall precursors having different glycine branch lengths (penta-, tri-, or monoglycine), while only cross-link strands bearing complete pentaglycine branch. This hypothesis never tested because necessary...
The main differences of health-promoting compounds and antioxidant activity whole individual edible parts baby mustard were demonstrated.
Scope Intestinal flora plays a critical role in the development of . Rutin is natural flavonoid with potential prebiotic effects on regulating intestinal composition that beneficial for host health. Therefore, this study hypothesizes rutin supplementation has high‐fat‐diet (HFD)‐induced obesity and metabolic disorder through modulation mice. Methods results The obesity‐alleviating property using 6‐week‐old C57BL/6J male mice fed HFD or without 16 weeks investigated. effectively reduces...
Abstract A β-Ga2O3 trench Schottky barrier diode (T-SBD) with double-field-plates terminal and cost effective Al2O3/SiN dielectric layer is fabricated. Owing to the shielding effect of MIS (Metal-Insulator-Semiconductor) structure, compared Conventional SBD (C-SBD), breakdown voltage (BV) T-SBD improved from 700 V (C-SBD) 1380 (TSBD) specific on-resistance (Ron,sp) being 6.06 mΩ·cm2. Compared C-SBD, Baliga’s Figure Merit for 131 MW/cm2 314 (T-SBD). Moreover, leakage current significantly...
Staphylococcus aureus is a Gram-positive pathogen with an unusual mode of cell division in that it divides orthogonal rather than parallel planes. Through selection using moenomycin, antibiotic proposed to target peptidoglycan glycosyltransferases (PGTs), we have generated resistant mutants containing single point mutation the active site PGT domain essential (PG) biosynthetic enzyme, PBP2. Using free polymerization assays, show this alters activity so much shorter PG chains are made. The...