A5100354341- Gut microbiota and health
- Probiotics and Fermented Foods
- Peptidase Inhibition and Analysis
- Analytical chemistry methods development
- Carbohydrate Chemistry and Synthesis
- Clostridium difficile and Clostridium perfringens research
- Autophagy in Disease and Therapy
- Protein Degradation and Inhibitors
- Antifungal resistance and susceptibility
- Antibiotic Resistance in Bacteria
- Multiple Myeloma Research and Treatments
- Pesticide Residue Analysis and Safety
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Diet and metabolism studies
- Gastrointestinal motility and disorders
- Cellular transport and secretion
- Protein Tyrosine Phosphatases
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Advanced biosensing and bioanalysis techniques
- Cannabis and Cannabinoid Research
- Sirtuins and Resveratrol in Medicine
- Lysosomal Storage Disorders Research
- Vibrio bacteria research studies
Nanyang Technological University
2022-2024
First Affiliated Hospital of Zhengzhou University
2024
Second Xiangya Hospital of Central South University
2023
Fudan University
2012-2023
Xiangyang Central Hospital
2023
Central South University
2023
Zhongshan Hospital
2023
Hubei University of Arts and Science
2023
Shanghai Stomatological Hospital
2020
Heilongjiang Center for Tuberculosis Control and Prevention
2018
Patients with type 1 diabetes (T1D) and 2 (T2D) present intestinal disturbances. Recent epidemiological data have showed that, worldwide, over half of newly diagnosed T1D patients were adults. However, the gut microbial alterations in adult-onset are unclear. We aimed to identify signatures microbiota metabolites systematically, comparing T2D healthy controls (HCs).
PGN_MS2: an in silico PGN library for automated LC-MS/MS spectral deconvolution of bacterial peptidoglycan fragments (PGNs).
Peptidoglycan (PG), an essential exoskeletal polymer in bacteria, is a well-known antibiotic target. PG polymerization requires the action of bacterial transglycosylases (TGases), which couple incoming glycosyl acceptor to donor. Interfering with TGase activity can interrupt assembly. Existing inhibitors like moenomycin and Lipid II analogues always occupy active sites; other strategies interfere proper elongation have not been widely exploited. Inspired by natural 1,6-anhydro-MurNAc termini...
Abstract A novel molecularly imprinted polymer that could selectively recognize tetracyclines in milk powder was synthesized using a metal–organic framework as support material, tetracycline template molecule, and 3‐aminophenylboronic acid functional monomer cross‐linking agent. The characterized by Fourier transform infrared spectrometry, transmission electron microscopy, X‐ray diffractometry, thermogravimetric analysis, N 2 adsorption/desorption measurements. adsorption isotherms,...
Antimicrobial resistance (AMR) is a long-term public health challenge worldwide, and it increasingly recognized to be heteroresistance phenomenon in an isogenic bacterial population. When the minority population of resistant bacteria with strong AMR not handled time, such subpopulation can enriched leading further development AMR. However, conventional studies based on ensemble-averaged data from large fail characterize heterogeneity. In this work, we develop method using plasmonic...
T cell development and homeostasis are both regulated by TCR signals. Protein phosphorylation dephosphorylation, which catalyzed protein kinases phosphatases, respectively, serve as important switches controlling multiple downstream pathways triggered recognition of Ags. It has been well documented that tyrosine phosphatases involved in negative regulation proximal signaling. However, how signals terminated or attenuated the distal signaling is largely unknown. We investigated function...
Candida albicans, the major fungal pathogen in humans, is under strong influence of bacterial peptidoglycan fragments to undergo yeast-to-hyphae transition, a key virulent step C. albicans pathogenesis and infections. However, due synthetic difficulties obtaining for biological studies, mechanistic details how recognizes uptakes these have not been well elucidated. Notably, previous works solely focused on ligand, muramyl dipeptide (MDP), despite its poor hyphal-inducing activity albicans....
The key virulent characteristic of Candida albicans, the major fungal pathogen in humans, lies its ability to switch between benign yeast state and invasive hyphal form upon exposure specific stimuli. Among numerous hyphal-inducing signals, bacterial peptidoglycan fragments (PGNs) represent most potent inducers C. albicans growth. sole adenylyl cyclase Cyr1 is a known sensor for PGNs activates downstream signaling growth, yet molecular details PGN-Cyr1 interactions have remained unclear. In...
Abstract Maresin-2, a specialized pro-solving mediator of inflammation has been consolidated to be novel cytokine fine-tuning inflammatory cascade. However, the underlying molecular basis is still largely unknown. Focused on cardiac dysfunction and remodeling, we employed in vivo- vitro- based genome editing methodology tools including adenosine associated virus, lenti-virus, plasmid transfection, CRISPR-Cas9 for investigation. As suggested, exogenous maresin-2 supplement facilitated...
This study aimed to investigate the epidemiological characteristics and trends over time of carbapenemase-producing (e.g., KPC, NDM, VIM, IMP, OXA-48) Gram-negative bacteria (CPGNB). Non-duplicated multi-drug resistant (MDRGNB) were collected from First Affiliated Hospital Zhengzhou University April 2019 February 2023. Species identification each isolate was performed using Vitek2 system confirmed by matrix-assisted laser desorption ionization-time flight mass spectrometry according...
Gut microbiota-derived peptidoglycan fragments (PGNs) are key signaling molecules that regulate multiple aspects of the host health. Yet exact structures natural PGNs in hosts have not been fully elucidated. Herein, we developed an LC-HRMS/MS analytical platform for global quantification and profiling PGN subtypes gut sera, unexpectedly revealing abundance PGN-derived saccharide moieties do resemble canonical ligands mammalian NOD1/2 receptors. Focusing on disaccharide GlcNAc-MurNAc (GM), a...
Abstract Peptidoglycan is an essential exoskeletal polymer present across all bacteria. The gut microbiota-derived peptidoglycan fragments (PGNs) are increasingly recognized as key effector molecules that impact host biology, offering attractive yet untapped potential to combat microbiome-associated diseases in humans. Unfortunately, comprehensive profiling of bacteria has been hampered by the lack a robust and automated analysis workflow. Currently, PGN identification still relies on manual...
NuSAP functions as a microtubule stabilizer. In this study, we dissect the function of in kinetochore microtubules and identify MCAK novel binding partner NuSAP. tightly regulates localization depolymerization activity MCAK. Moreover, demonstrate that Aurora B significantly enhances interaction with modulates effects on Our results elucidate an underlying mechanism which controls dynamics spatially temporally by modulating MCAK, kinase-dependent manner.
Abstract Background Despite significant therapeutic advances over the last decade, Multiple myeloma remains an incurable disease. Pomalidomide is a second-generation Immunomodulatory drug that commonly used to treat patients with relapsed/refractory multiple myeloma. However, approximately half of exhibit resistance pomalidomide treatment. While previous studies have identified Cereblon as primary target drugs’ anti-myeloma activity, it crucial explore additional mechanisms are currently...
Despite significant therapeutic advances over the last decade, multiple myeloma remains an incurable disease. Pomalidomide is third Immunomodulatory drug that commonly used to treat patients with relapsed/refractory myeloma. However, approximately half of exhibit resistance pomalidomide treatment. While previous studies have identified Cereblon as a primary target drugs' anti-myeloma activity, it crucial explore additional mechanisms are currently less understood.To comprehensively...