A5034496613- Autoimmune and Inflammatory Disorders Research
- Chemical Synthesis and Analysis
- Adolescent and Pediatric Healthcare
- Ubiquitin and proteasome pathways
- Click Chemistry and Applications
- 14-3-3 protein interactions
- thermodynamics and calorimetric analyses
- bioluminescence and chemiluminescence research
- Fatty Acid Research and Health
- Computational Drug Discovery Methods
- Vascular Anomalies and Treatments
- Acute Lymphoblastic Leukemia research
- Inflammasome and immune disorders
- Radiopharmaceutical Chemistry and Applications
- Microbial Natural Products and Biosynthesis
- Kawasaki Disease and Coronary Complications
- Cell Image Analysis Techniques
- Cholesterol and Lipid Metabolism
- Phytochemistry and Bioactive Compounds
- Otitis Media and Relapsing Polychondritis
- Gut microbiota and health
- Ecocriticism and Environmental Literature
- Chronic Kidney Disease and Diabetes
- Liver Disease Diagnosis and Treatment
- Neonatal Respiratory Health Research
Merck & Co., Inc., Rahway, NJ, USA (United States)
2019-2022
Penn State Milton S. Hershey Medical Center
2022
Novartis (United States)
2015-2021
University of California, San Francisco
2021
Amgen (United States)
2021
Massachusetts General Hospital
1982-2020
Harvard University Press
2013
Shriners Hospitals for Children - Springfield
2012
Shriners Hospitals for Children - Erie
2000-2011
Massachusetts Institute of Technology
2002-2005
Significance The spread of multidrug-resistant infections demands antibiotic development. Although new antibiotics targeting gram-positive bacteria have been developed, it has more than 50 y since a class gram-negative approved for clinical use. strong outer membrane permeability barrier makes difficult compounds to reach intracellular targets, contributing greatly the lack this microbes. Here, we describe compound, MRL-494, that inhibits assembly proteins into by an essential member...
To formulate consensus treatment plans (CTPs) for induction therapy of newly diagnosed proliferative lupus nephritis (LN) in juvenile systemic erythematosus (SLE).A structured formation process was employed by the members Childhood Arthritis and Rheumatology Research Alliance after considering existing medical evidence current approaches.After an initial Delphi survey (response rate = 70%), a 2-day conference, 2 followup surveys rates 63-79%), achieved limited set CTPs addressing LN. These...
Periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) syndrome is the most common periodic fever in children. There considerable heterogeneity management strategies a lack of evidence-based treatment guidelines. Consensus plans (CTPs) are standardized regimens that derived based upon best available evidence current practices way to enable comparative effectiveness studies identify optimal therapy less costly execute than randomized, double blind placebo controlled...
A new 4-(N,N-dimethylamino) phthalimide-based environment-sensitive fluorescent building block for solid phase peptide synthesis, has been synthesized and incorporated into peptides. Peptides incorporating this residue show great potential biological applications in sensing protein/protein interactions.
Objective. The pathophysiology of shrinking lung syndrome (SLS) is poorly understood. We sought to define the structural basis for this condition through study pulmonary mechanics in affected patients. Methods. Since 2007, most patients evaluated SLS at our institutions have undergone standardized respiratory testing including esophageal manometry. analyzed these studies physiological abnormalities driving restriction. Chest computed tomography data were post-processed quantify volume and...
Although current antiretroviral therapy can control HIV-1 replication and prevent disease progression, it is not curative. Identifying mechanisms that lead to eradication of persistent viral reservoirs in people living with (PLWH) remains an outstanding challenge achieving cure. Utilizing a phenotypic screen, we identified novel chemical class capable killing infected peripheral blood mononuclear cells. Tool compounds ICeD-1 ICeD-2 ("inducer cell death-1 2"), optimized for potency...
We present the chemical and biological synthesis of caged phosphoproteins using in vitro nonsense codon suppression methodology. Specifically, phosphoamino acid analogues serine, threonine, tyrosine with a single photocleavable o-nitrophenylethyl caging group were synthesized as amino acyl tRNA adducts for insertion into full-length proteins. For this purpose, novel phosphitylating agent was developed. The successful incorporation these bulky charged acids α-subunit nicotinic acetyl choline...
Three 1-(2-nitrophenyl)ethyl-caged phospho-amino acids have been synthesized for use in standard Nα-fluorenylmethoxycarbonyl-based solid-phase peptide synthesis (SPPS). The most common naturally occurring acids, serine, threonine, and tyrosine, were prepared as protected caged building blocks by modification with a unique phosphitylating reagent. In previous work, phospho-peptides made using an interassembly approach (Rothman, D. M.; Vazquez, M. E.; Vogel, E. Imperiali, B. Org. Lett. 2002,...
An interassembly approach for the synthesis of peptides containing 1-(2-nitrophenyl)ethyl-caged phosphoserine, -threonine, and -tyrosine has been developed. Photochemical uncaging these releases 2-nitrophenylethyl protecting group to afford corresponding phosphopeptide. The described herein are based on phosphorylation sites kinases involved in cell movement or cycle regulation demonstrate versatility method compatibility with polypeptides, including a variety encoded amino acids.
Type 2 diabetes (T2D) is caused by loss of pancreatic β-cell mass and failure the remaining β-cells to deliver sufficient insulin meet demand. β-Cell glucolipotoxicity (GLT), which refers combined, deleterious effects elevated glucose fatty acid levels on function survival, contributes T2D-associated failure. Drugs mechanisms that protect from GLT stress could potentially improve metabolic control in patients with T2D. In a phenotypic screen seeking low-molecular-weight compounds protected...
To investigate the incidence and risk factors for hypogammaglobulinaemia infectious complications associated with rituximab treatment in childhood-onset rheumatic diseases.We performed a single-centre retrospective study of patients (n = 85) treated at Boston Children's Hospital (BCH) from 2009 to 2019. Study subjects included (ages 6-24 years) who received disease.New-onset developed 23 (27.1%) within 18 months induction treatment. Twenty-two (25.9%) least one complication following first...
The assembly of chemogenetic libraries composed chemical probes provides tremendous value to biomedical research, but requires substantial effort ensure diversity as well quality the contents. We are assembling a library by data mining and crowdsourcing institutional expertise. sharing our methodology, lessons learned, disclosing current collection 4186 compounds with their primary annotated gene targets.