A5087305762- Chalcogenide Semiconductor Thin Films
- Biochemical Acid Research Studies
- Luminescence Properties of Advanced Materials
- Malaria Research and Control
- Crystal Structures and Properties
- Quantum Dots Synthesis And Properties
- Amino Acid Enzymes and Metabolism
- Chemical Synthesis and Analysis
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Metabolism and Genetic Disorders
- Nicotinic Acetylcholine Receptors Study
- Biochemical and biochemical processes
- Solid-state spectroscopy and crystallography
- Alcoholism and Thiamine Deficiency
- X-ray Diffraction in Crystallography
- Gas Sensing Nanomaterials and Sensors
- Metabolomics and Mass Spectrometry Studies
- Chromatography in Natural Products
- Copper Interconnects and Reliability
- Coral and Marine Ecosystems Studies
- Research on Leishmaniasis Studies
- Masonry and Concrete Structural Analysis
- Advanced Semiconductor Detectors and Materials
- Bioactive Compounds and Antitumor Agents
Instituto Federal Catarinense
2022
Franklin & Marshall College
2014-2021
Lancaster City Council
2021
Brandeis University
2006-2016
Johns Hopkins University
2013
University of Michigan
2008-2009
Rutgers, The State University of New Jersey
2008-2009
Indiana University – Purdue University Indianapolis
2009
Howard Hughes Medical Institute
2006
Harvard University
2006
Accumulation of misfolded protein in the endoplasmic reticulum (ER) triggers an adaptive stress response—termed unfolded response (UPR)—mediated by ER transmembrane kinase and endoribonuclease inositol-requiring enzyme–1α (IRE1α). We investigated UPR signaling events mice absence proapoptotic BCL-2 family members BAX BAK [double knockout (DKO)]. DKO responded abnormally to tunicamycin-induced liver, with extensive tissue damage decreased expression IRE1 substrate X-box–binding 1 its target...
The optical absorption of amorphous electrochromic display layers is explained as small polaron absorption. necessary W5+ 5d‐electron localization favored by the lattice disorder. This concluded from change properties to free‐electron‐like behavior upon crystallization layers. increased electrocoloration stability and blue shift peak in Mo‐doped films can also be within model given.
A series of tryptophan analogues has been introduced into the binding site regions two ion channels, ligand-gated nicotinic acetylcholine and serotonin 5-HT3A receptors, using unnatural amino acid mutagenesis heterologous expression in Xenopus oocytes. cation−π interaction between Trp183 channel 5-HT3AR is identified for first time, precisely locating ligand-binding this receptor. The energetic contribution observed a primary ammonium estimated to be approximately 4 kcal/mol, while...
Abstract Nuclear magnetic resonance spectra of Cu 63 and Al 27 , Ga 69 In 115 have been recorded in ternary I B –III–VI 2 semiconductors, which crystallize the tetragonal chalcopyrite structure. electric quadrupole coupling constants determined are discussed connection with structural parameters these compounds, were by X‐ray diffraction.
Mycobacterium tuberculosis (Mtb) employs multiple strategies to evade host immune responses and persist within macrophages. We have previously shown that the cell envelope-associated Mtb serine hydrolase, Hip1, prevents robust macrophage activation dampens pro-inflammatory responses, allowing delay detection accelerate disease progression. now provide key mechanistic insights into molecular biochemical basis of Hip1 function. establish is a protease with activity against protein peptide...
Tyrosine side chains participate in several distinct signaling pathways, including phosphorylation and membrane trafficking. A nonsense suppression procedure was used to incorporate a caged tyrosine residue place of the natural at position 242 inward rectifier channel Kir2.1 expressed Xenopus oocytes. When kinases were active, flash decaging led both decreased K+ currents also substantial (15–26%) decreases capacitance, implying net endocytosis. dominant negative dynamin mutant completely...
We present the chemical and biological synthesis of caged phosphoproteins using in vitro nonsense codon suppression methodology. Specifically, phosphoamino acid analogues serine, threonine, tyrosine with a single photocleavable o-nitrophenylethyl caging group were synthesized as amino acyl tRNA adducts for insertion into full-length proteins. For this purpose, novel phosphitylating agent was developed. The successful incorporation these bulky charged acids α-subunit nicotinic acetyl choline...
The nonsense codon suppression technique was used to incorporate o-nitrobenzyl cysteine or tyrosine (caged Cys Tyr) into the 9′ position of M2 transmembrane segment γ-subunit muscle nicotinic ACh receptor expressed in Xenopusoocytes. caged amino acids replaced an endogenous Leu residue that has been implicated channel gating. ACh-induced current increased substantially after ultraviolet (UV) irradiation remove caging group. This represents first successful incorporation a protein vivo and...
Benzaldehyde lyase (BAL) catalyzes the reversible cleavage of (R)-benzoin to benzaldehyde utilizing thiamin diphosphate and Mg2+ as cofactors. The enzyme is important for chemoenzymatic synthesis a wide range compounds via its carboligation reaction mechanism. In addition principal functions, BAL can slowly decarboxylate aromatic amino acids such benzoylformic acid. It also intriguing mechanistically due paucity acid−base residues at active center that participate in proton transfer steps...
Benzoylformate decarboxylase (BFDC) is a thiamin diphosphate- (ThDP-) dependent enzyme acting on aromatic substrates. In addition to its metabolic role in the mandelate pathway, BFDC shows broad substrate specificity coupled with tight stereo control carbon−carbon bond-forming reverse reaction, making it useful biocatalyst for production of chiral α-hydroxy ketones. The reaction methyl benzoylphosphonate (MBP), an analogue natural benzoylformate, results formation stable...
Significance The transcription factor T-bet (Tbox protein expressed in T cells), a master regulator of T-cell lineage commitment, is member the Tbox family but coordinately regulates many more genes than other proteins. How simultaneously recognizes distant elements that may be thousands base pairs apart unknown. We have determined crystal structure DNA binding domain complexed with 24-bp palindromic DNA. shows dimer where each monomer binds to two independent molecules. Fluorescence-based...
Benzaldehyde lyase (BAL) from Pseudomonas putida is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the breakdown of (R)-benzoin. Here we report point mutant, BAL A28S, not only decarboxylation benzoylformate but, like decarboxylase (BFDC), also inactivated by analogues methyl benzoylphosphonate (MBP) and (BP). The latter has no effect on wild-type BAL, inactivation A28S variant shown to result phosphorylation newly introduced serine residue. This lends support proposal an...
A relatively high-affinity inhibitor of FIKK kinase from the malaria parasite Plasmodium vivax was identified by in vitro assay recombinant kinase. The family is unique to parasitic organisms Apicomplexan order and has been shown be critical parasites. domain expressed screened against a small molecule library, revealing number tyrosine inhibitors that block activity. tyrphostins further investigated, begin exploring pharmacophore. Finally, emodin as inhibitor, identifying this...
The composition of anodic films on Hg0.8Cd0.2Te is reinvestigated and it argued that the use ion sputtering (e.g., as a depth profiling technique) significantly perturbs chemical structure these within probing depths electron spectroscopy (AES XPS). Evidence for this argument given by detailed study HgTeO3 which suggested dominant mercury constituent Hg1−xCdxTe. was grown horizontal Bridgman method obtained in triclinic modification. Its XPS data were compared with those electrochemically...
The mechanism of the enzyme benzoylformate decarboxylase (BFDC), which carries out a typical thiamin diphosphate (ThDP)-dependent nonoxidative decarboxylation reaction, was studied with chromophoric alternate substrate (E)-2-oxo-4(pyridin-3-yl)-3-butenoic acid (3-PKB). Addition 3-PKB resulted in appearance two transient intermediates formed consecutively, first one to be predecarboxylation ThDP-bound intermediate λmax at 477 nm, and second corresponding postdecarboxylation enamine 437 nm....