A5061315099- Chronic Obstructive Pulmonary Disease (COPD) Research
- Metabolomics and Mass Spectrometry Studies
- Asthma and respiratory diseases
- Protein Structure and Dynamics
- Advanced Chemical Sensor Technologies
- Allelopathy and phytotoxic interactions
- Plant Pathogens and Fungal Diseases
- Chemical synthesis and alkaloids
- Fungal Plant Pathogen Control
- Respiratory Support and Mechanisms
- Phytochemistry and Biological Activities
- Lipid Membrane Structure and Behavior
- NMR spectroscopy and applications
- Molecular spectroscopy and chirality
- Advanced NMR Techniques and Applications
- COVID-19 Clinical Research Studies
- Enzyme Structure and Function
- Electron Spin Resonance Studies
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Estrogen and related hormone effects
- Neuropeptides and Animal Physiology
- Biochemical effects in animals
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
National Research Council
2015-2024
Istituto di Chimica Biomolecolare
2013-2024
National Academies of Sciences, Engineering, and Medicine
2011-2024
Institute of Protein Biochemistry
2016-2023
Radboud University Medical Center
2023
Radboud University Nijmegen
2023
University of Milan
2022
IRCCS Ospedale San Raffaele
2021
Vita-Salute San Raffaele University
2020
San Raffaele University of Rome
2015
Abstract Objectives The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to date, the epidemic has gradually spread 209 countries worldwide with more than 1.5 million infected people and 100,000 deaths. Amplification viral RNA by rRT-PCR serves as gold standard for confirmation infection, yet it needs a long turnaround time (3–4 h generate results) shows false-negative rates large 15%–20%. In addition, need certified laboratories, expensive equipment trained personnel...
Nothing is known about the potential implication of gut microbiota in skeletal muscle disorders. Here, we provide evidence that fecal composition along with circulating levels short-chain fatty acids (SCFAs) and related metabolites are altered mdx mouse model Duchenne muscular dystrophy (DMD) compared healthy controls. Supplementation sodium butyrate (NaB) mice rescued strength autophagy, prevented inflammation associated excessive endocannabinoid signaling at CB1 receptors to same extent as...
<h3>Background</h3> Metabolomics could provide new insights into the pathophysiology of cystic fibrosis (CF) by identifying profiles endogenous metabolites. <h3>Objectives</h3> To investigate whether metabolomics exhaled breath condensate discriminate between patients with unstable CF, stable CF and healthy subjects, selected metabolites were responsible for between-group differences. <h3>Methods</h3> Twenty-nine 24 31 subjects (age 9–24 years) participated in a cross-sectional study. was...
Background: Endothelial dysfunction has a key role in the pathogenesis of coronavirus disease 2019 (COVID-19) and its disabling complications. We designed case-control study to assess alterations endothelium-dependent flow-mediated dilation (FMD) among convalescent COVID-19 patients. Methods: patients referred Pulmonary Rehabilitation Unit within 2 months from swab test negativization were consecutively evaluated for inclusion compared controls matched age, gender, cardiovascular risk...
In spite of the huge advancements in both diagnosis and interventions, hormone refractory prostate cancer (HRPC) remains a major hurdle (PCa). Metabolic reprogramming plays key role PCa oncogenesis resistance. However, dynamics between metabolism are not fully understood. Here, we demonstrate that two multi-target natural products, cannabidiol (CBD) cannabigerol (CBG), suppress HRPC development TRansgenic Adenocarcinoma Mouse Prostate (TRAMP) model by metabolic oncogenic signaling....
Exhaled breath condensate (EBC) is a noninvasive method for the study of airway lining fluid. Nuclear magnetic resonance (NMR) spectroscopy can provide biochemical profiles metabolites in biological samples. The aim present was to validate NMR metabonomic analysis EBC adults, assessing role pre-analytical variables (saliva and disinfectant contamination) potential clinical feasibility. In total, 36 paired saliva samples, obtained from healthy subjects, laryngectomised patients chronic...
Nuclear magnetic resonance (NMR)-based metabolomics separates exhaled breath condensate (EBC) profiles of patients affected by pulmonary disease from those healthy subjects. Here we show the discriminatory ability NMR-based in separating exposed to same risk factor, namely, smoking habit smoking-related diseases. Fifty duplicated EBC samples a cohort current smokers without chronic obstructive (COPD, henceforth HS), COPD smokers, and subjects with established Langerhans cell histiocytosis...
Asthma prevalence in Italy is on the rise and estimated to be over 6% of general population. The diagnosis asthma can challenging elusive, especially children last two decades has brought evidences that not a single disease but consists various phenotypes. Symptoms underestimated by patient or underreported clinician physical signs scanty. Usual objective measures, like spirometry, are necessary sometimes significant. Despite proper treatment, very severe condition (even leading death),...
A new antifungal 6-substituted 2H-pyran-2-one, named viridepyronone, has been isolated from a cultural filtrate of strain Trichoderma viride showing antagonistic activity in vitro toward Sclerotium rolfsii, which is the causal agent crown and stem rot artichoke. Viridepyronone was characterized as 6-(4-oxopentyl)-2H-pyran-2-one 2 with spectroscopic methods. Bioassays showed that viridepyronone had good against S. its minimum inhibitory concentration (over 90% inhibition) found to be 196...
Stagonospora cirsii, a fungal pathogen isolated from Cirsium arvense and proposed as potential mycoherbicide of this perennial noxious weed, produces phytotoxic metabolites in liquid solid cultures. Recently, the main metabolite, stagonolide (1), with interesting properties, was culture characterized new nonenolide. In present work same fungus, grown culture, exhibited an increased capacity to produce nonenolides. Five nonenolides, named stagonolides B−F (2–6), were using spectroscopic...
Exhaled breath condensate (EBC) collection is a noninvasive method to investigate lung diseases. EBC usually collected with commercial/custom-made condensers, but the optimal condensing temperature often unknown. As such, physical and chemical properties of exhaled metabolites should be considered when setting temperature, therefore requiring validation standardization collecting procedure. frequently used in nuclear magnetic resonance (NMR)-based metabolomics, which unambiguously recognizes...
Significance Waste nitrogen is converted into urea by the healthy liver. Systemic ammonia elevated in patients with inherited urea-cycle disorders or acute and chronic liver diseases. Hyperammonemia a challenging medical condition that holds high risks of mortality irreversible brain damage. Current treatments are often inadequate. Autophagy lysosomal recycling pathway plays major role metabolism. Here, we found autophagy involved homeostasis vivo enhancement hepatic increases ureagenesis...
A multicenter study to compare results of BRAHMS Kryptor PCT with those obtained using four BRAHMS-partnered procalcitonin (PCT) automated immunoassays (DiaSorin Liaison, BioMérieux Vidas, Roche Cobas E601 and Siemens Advia Centaur) the Diazyme immunotubidimetric assay implemented on clinical chemistry platforms (Abbott Architect c16000, 2400, C501 Beckman Coulter AU5800). One hundred serum samples from in-patients values between 0.10 58.7 ng/mL were divided into aliquots tested nine...
Background: Growing evidence points to a key role of endothelial dysfunction in the pathogenesis COVID-19. In this study, we evaluated changes endothelium-dependent flow-mediated dilation (FMD) cohort convalescent COVID-19 patients undergoing pulmonary rehabilitation (PR). Methods: After swab test negativization, referring post-acute care facility for PR were consecutively screened inclusion. Study procedures performed at time hospitalization and discharge. Results: We enrolled 82 (85.4%...