A5068082099- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Wastewater Treatment and Nitrogen Removal
- Tissue Engineering and Regenerative Medicine
- Plant Micronutrient Interactions and Effects
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Agronomic Practices and Intercropping Systems
- Cancer-related gene regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Cancer Research and Treatments
- Heat shock proteins research
- Coastal wetland ecosystem dynamics
- Amino Acid Enzymes and Metabolism
- Melanoma and MAPK Pathways
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Nematode management and characterization studies
Institute of Genetics and Biophysics
2015-2024
National Research Council
2002-2015
I.R.C.C.S. Oasi Maria SS
2005
GTx (United States)
1998-2004
TCL (China)
1999-2004
Weatherford College
2000
SUMMARY Symbiotic nitrogen fixation is carried out in specialized organs, the nodules, whose formation induced on leguminous host plants by bacteria belonging to family Rhizobiaceae . Nodule development a complex multistep process, which requires continued interaction between two partners and thus exchange of different signals metabolites. NH 4 + not only primary product but also main regulator symbiosis: either as ammonium after conversion into organic compounds, it regulates most stages...
Metabolites are emerging as key mediators of crosstalk between metabolic flux, cellular signaling, and epigenetic regulation cell fate. We found that the nonessential amino acid L-proline (L-Pro) acts a signaling molecule promotes conversion embryonic stem cells into mesenchymal-like, spindle-shaped, highly motile, invasive pluripotent cells. This embryonic-stem-cell-to-mesenchymal-like transition (esMT) is accompanied by genome-wide remodeling H3K9 H3K36 methylation status. Consistently,...
Floating spheroidal aggregates of mouse embryonic stem cells can develop into polarized/elongated organoids, namely gastruloids. We set up a high-performing assay to measure gastruloid formation efficiency (GFE), and found that GFE decreases as pluripotency progresses from naive (GFE ≥ 95%) primed = 0) state. Specifically, we show EpiSCs fail generate proper cell aggregates, while early-primed EpiLCs aggregate but eventually elongated Moreover, characterized proline-induced (PiCs),...
The molecular mechanisms controlling mouse embryonic stem cell (ESC) metastability, i.e. their capacity to fluctuate between different states of pluripotency, are not fully resolved. We developed and used a novel automation platform, the Cellmaker, screen library metabolites on two ESC-based phenotypic assays (i.e. proliferation colony phenotype) identified metabolically related amino acids, namely l-proline (l-Pro) l-ornithine (l-Orn), as key regulators ESC metastability. Both compounds,...
Increasing evidence indicates that metabolism is implicated in the control of stem cell identity. Here, we demonstrate embryonic (ESC) behaviour relies on a feedback loop involves non-essential amino acid L-Proline (L-Pro) modulation Gcn2-Eif2α-Atf4 starvation response (AAR) pathway turn regulates L-Pro biosynthesis. This regulatory generates highly specific intrinsic shortage restricts proliferation tightly packed domed-like ESC colonies and safeguards Indeed, alleviation this nutrient...
Abstract Known molecular determinants of developmental plasticity are mainly transcription factors, while the extrinsic regulation this process has been largely unexplored. Here we identify Cripto as one earliest epiblast markers and a key extracellular determinant naive primed pluripotent states. We demonstrate that sustains mouse embryonic stem cell (ESC) self-renewal by modulating Wnt/β-catenin, whereas it maintains (EpiSC) human ESC pluripotency through Nodal/Smad2. Moreover, provide...
The initial conditions for morphogenesis trigger a cascade of events that ultimately dictate structure and functions tissues organs. Here we report surface nanopatterning can control the assembly focal adhesions, hence guiding human mesenchymal stem cells (hMSCs) through process self-organization differentiation. This self-sustains, leading to development macroscopic with molecular profiles microarchitecture reminiscent embryonic tendons. Therefore, material surfaces be in principle...
Abstract Collagen prolyl hydroxylation (CPH), which is catalyzed by 4-hydroxylase (P4H), the most prevalent posttranslational modification in humans and requires vitamin C (VitC). Here, we demonstrate that CPH acts as an epigenetic modulator of cell plasticity. Increased induced global DNA/histone methylation pluripotent stem tumor cells promoted state transition (CST). Interfering with either genetic ablation P4H subunit alpha-2 (P4HA2) or pharmacologic treatment reverted changes...
The synthetic cortisol analog budesonide (BUD) is an essential drug employed to manage chronic inflammatory diseases in humans, mainly those involving gastroenteric and airway mucosa, such as rhinitis, laryngitis, bronchitis, esophagitis, gastritis, colitis, with high levels of success. As a glucocorticoid, BUD prevents the expression pro-inflammatory cytokines/chemokines recruitment immune cells into inflamed mucosa. However, emerging evidences indicates that BUD, unlike classical...
The synthetic cortisol analog budesonide (BUD) is an essential drug employed to manage chronic inflammatory diseases in humans, mainly those involving gastroenteric and airway mucosa, such as rhinitis, laryngitis, bronchitis, esophagitis, gastritis, colitis, with high levels of success. As a glucocorticoid, BUD prevents the expression pro-inflammatory cytokines/chemokines recruitment immune cells into inflamed mucosa. However, emerging evidence indicates that BUD, unlike classical...
By means of a detailed ultrastructural analysis nodules induced by Rhizobium etli on the roots Phaseolus vulgaris, we observe that development host-invaded cells is not synchronous. An accumulation mitochondria was found in freshly invaded host cells, containing only few symbiosomes (SBs) are released from highly branched intracellular ramification infection threads. Moreover, besides fusion between SB membrane with secretory vesicles, also great number fusions outer leaflets adjoining...
In the present study, phenotype of melanoma cells resistant to dabrafenib (a B-RAF inhibitor) was investigated, shed more light on resistance inhibition. Melanoma were generated using 3 different cell lines, A375, 397 and 624.38, all carrying B-RAFV600E, they characterized by cytofluorometric analysis, Ion Torrent technology, immunofluorescence biochemistry. All dabrafenib-resistant showed, in addition a re-activation MAPK signaling, morphological changes compared their sensitive...
During development of root nodules, Rhizobium bacteria differentiate inside the invaded plant cells into N 2 -fixing bacteroids. Terminally differentiated bacteroids are unable to grow using ammonia (NH 3 ) produced therein by nitrogenase complex. Therefore, nitrogen assimilation activities bacteroids, including ammonium 4 + uptake activity, expected be repressed during symbiosis. By sequence homology R. etli amtB (ammonium transport) gene was cloned and sequenced. As previously shown for...
Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is the most lethal cancer with an aggressive metastatic phenotype and very poor clinical prognosis. Interestingly, a lower occurrence of PDAC has been described in individuals severe long-standing asthma. Here we explored potential link between glucocorticoid (GC) budesonide, first-line therapy to treat Methods We tested effect budesonide classical GCs on morphology, proliferation, migration invasiveness patient-derived cells...
Summary We report here that ntrB and ntrC genes of Rhizobium leguminosarum biovar phaseoli are cotranscribed with an open reading frame (called 0RF1) unknown Unction. The promoter region the 0RF1‐ntrB‐ntrC operon was mapped immediately upstream ORF1 two in vivo transcription initiation sites were identified, both preceded by −35/−10 consensus sequences. Some major aspects differentiate ft from enteric nitrogen regulatory system: ntrBC 0RF1 which is homologous to ORF located R. capsulatus fis...
Abstract Dystrophies are characterized by progressive skeletal muscle degeneration and weakness as consequence of their molecular abnormalities. Thus, new drugs for restoring deterioration critically needed. To identify alternative compounds with a functional role in myogenesis, we screened library pharmacologically active selected the small molecule 6-bromoindirubin-3′-oxime (BIO) an inhibitor myoblast proliferation. Using C2C12 cells, examined BIO’s effect during proliferation...
Skeletal muscle repair relies on heterogeneous populations of satellite cells (SCs). The mechanisms that regulate SC homeostasis and state transition during activation are currently unknown. Here, we investigated the emerging role non-genetic micro-heterogeneity, i.e., intrinsic cell-to-cell variability a population, in this process. We demonstrate micro-heterogeneity membrane protein CRIPTO mouse-activated SCs (ASCs) identifies metastable cell states allow rapid response population to...