A5031406092- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Hepatitis C virus research
- Pancreatic function and diabetes
- Cancer Genomics and Diagnostics
- Phagocytosis and Immune Regulation
- Hedgehog Signaling Pathway Studies
- RNA modifications and cancer
- Immune cells in cancer
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- Cancer Mechanisms and Therapy
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- Systemic Lupus Erythematosus Research
- Peptidase Inhibition and Analysis
- Renal and related cancers
- Immunotherapy and Immune Responses
- Poxvirus research and outbreaks
- Cytomegalovirus and herpesvirus research
- Glioma Diagnosis and Treatment
- Telomeres, Telomerase, and Senescence
Instituto de Investigaciones Biomédicas Sols-Morreale
2016-2025
Universidad Autónoma de Madrid
2016-2025
Centro de Investigación Biomédica en Red de Cáncer
2022-2024
Instituto de Salud Carlos III
2022-2024
Instituto Ramón y Cajal de Investigación Sanitaria
2016-2024
Medical Research Network
2022-2023
Spanish National Cancer Research Centre
2013-2023
Instituto Cajal
2017-2023
Centro de Investigación Biomédica en Red
2018
Centre for Biomedical Network Research on Rare Diseases
2018
<h3>Background and aims</h3> The role of GATA factors in cancer has gained increasing attention recently, but the function GATA6 pancreatic ductal adenocarcinoma (PDAC) is controversial. amplified a subset tumours was proposed to be oncogenic, high levels are found well-differentiated associated with better patient outcome. By contrast, tumour-suppressive demonstrated using genetic mouse models. We aimed at clarifying PDAC. <h3>Design</h3> combined silencing overexpression PDAC cell lines...
Pancreatic ductal adenocarcinoma (PDAC) is a cancer of the exocrine pancreas with unmet medical need and strongly promoted by tumor-associated macrophages (TAM). The presence TAMs associated poor clinical outcome, their overall role, therefore, appears to be protumorigenic. "don't eat me" signal CD47 on cells communicates regulatory protein-α prevents phagocytosis. Thus, inhibition may offer new opportunity turn against PDAC cells, including stem (CSC), as exclusively tumorigenic population.
Objective The generation of acinar and ductal cells from human pluripotent stem (PSCs) is a poorly studied process, although various diseases arise this compartment. Design We designed straightforward approach to direct PSCs towards pancreatic organoids resembling progeny. Results Extensive phenotyping the not only shows appropriate marker profile but also ultrastructural, global gene expression functional hallmarks pancreas in dish. Upon orthotopic transplantation into immunodeficient mice,...
Significance Pancreatic ductal adenocarcinoma is one of the most malignant human tumors for which there are no efficacious therapeutic strategies. This tumor type characterized by an abundant desmoplastic stroma that promotes progression. Yet recent studies have shown physical or genetic elimination leads to more aggressive development. Here, we decided reprogram stromal tissue identifying and subsequently targeting genes responsible their protumorigenic properties. Comparative transcriptome...
Hypoparathyroidism is among the few hormonal insufficiency states not treated with replacement of missing hormone. Long-term conventional therapy vitamin D and analogs may lead to nephrocalcinosis renal insufficiency.Our objective was compare response once-daily vs. twice-daily PTH 1-34 treatment in children hypoparathyroidism.The study conducted at a clinical research center.Fourteen ages 4-17 yr chronic hypoparathyroidism were studied.This randomized cross-over trial, lasting 28 wk, which...
Cancer stem cells (CSC) are thought to play a major role in the development and metastatic progression of pancreatic ductal adenocarcinoma (PDAC), one deadliest solid tumors. Likewise, tumor microenvironment contributes critical support this setting, including from stromal tumor-associated macrophages (TAM) that contribute structural paracrine-mediated supports, respectively. Here, we show TAMs secrete IFN-stimulated factor ISG15, which enhances CSC phenotypes PDAC vitro vivo. ISG15 was...
Pancreatic ductal adenocarcinoma is one of the deadliest carcinomas and characterized by highly tumorigenic metastatic cancer stem cells (CSC). CSCs evade available therapies, which preferentially target proliferative more differentiated progenies, leaving behind as a putative source for disease relapse. Thus, to identify potentially effective treatment regimens, we screened established new compounds their ability eliminate in primary pancreatic (stem) vitro corresponding patient-derived...
<h3>Objective</h3> Cancer stem cells (CSCs) represent the root of many solid cancers including pancreatic ductal adenocarcinoma, are highly chemoresistant and cellular source for disease relapse. However mechanisms involved in these processes still need to be fully elucidated. Understanding implicated chemoresistance metastasis cancer is critical improving patient outcomes. <h3>Design</h3> Micro-RNA (miRNA) expression analyses were performed identify functionally defining epigenetic...
In order to elucidate how Hepatitis C Virus (HCV) interacts with polarized hepatocytes in vivo and HCV-induced alterations cellular function contribute HCV-associated liver disease, a more physiologically relevant hepatocyte culture model is needed. As such, NASA-engineered three-dimensional (3-D) rotating wall vessel (RWV) bioreactors were used effort promote differentiation of HCV-permissive Huh7 hepatoma cells.When cultured the RWV, cells became morphologically transcriptionally distinct...
<h3>Objectives</h3> The tumour stroma/microenvironment not only provides structural support for development, but more importantly it cues to cancer stem cells (CSCs) that regulate their self-renewal and metastatic potential. This is certainly true pancreatic ductal adenocarcinomas (PDAC), where tumour-associated fibroblasts, stellate immune create an abundant paracrine niche CSCs via microenvironment-secreted factors. Thus understanding the role stroma play in PDAC development CSC biology of...
Pancreatic ductal adenocarcinoma (PDAC) and other carcinomas are hierarchically organized, with cancer stem cells (CSC) residing at the top of hierarchy, where they drive tumor progression, metastasis, chemoresistance. As CSC non-CSC share an identical genetic background, we hypothesize that differences in epigenetics account for striking functional between these two cell populations. Epigenetic mechanisms, such as DNA methylation, play important role maintaining pluripotency regulating...
Abstract Pancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate approximately 7–9%. The ineffectiveness anti-PDAC therapies is believed to be due existence subpopulation tumor cells known as stem (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant metastatic capacities. Herein, we describe 2D in vitro system for long-term enrichment pancreatic CSCs that amenable biological CSC-specific studies. By...
Abstract Pancreatic cancer stem cells (PaCSCs) drive pancreatic tumorigenesis, chemoresistance and metastasis. While eliminating this subpopulation of would theoretically result in tumor eradication, PaCSCs are extremely plastic can successfully adapt to targeted therapies. In study, we demonstrate that increase expression interferon-stimulated gene 15 (ISG15) protein ISGylation, which essential for maintaining their metabolic plasticity. CRISPR-mediated ISG15 genomic editing reduces overall...
Objective The lysyl oxidase-like protein 2 (LOXL2) contributes to tumour progression and metastasis in different entities, but its role pancreatic ductal adenocarcinoma (PDAC) has not been evaluated immunocompetent vivo PDAC models. Design Towards this end, we used patient data sets, patient-derived xenograft vitro models, four conditional genetically-engineered mouse models (GEMMS) dissect the of LOXL2 PDAC. For GEMM-based studies, K-Ras +/LSL-G12D ; Trp53 LSL-R172H Pdx1-Cre mice (KPC) (KC)...