A5044436120- Particle physics theoretical and experimental studies
- Cancer Cells and Metastasis
- Quantum Chromodynamics and Particle Interactions
- Immunotherapy and Immune Responses
- Particle Detector Development and Performance
- T-cell and B-cell Immunology
- Helicobacter pylori-related gastroenterology studies
- High-Energy Particle Collisions Research
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Superconducting Materials and Applications
- Gastric Cancer Management and Outcomes
- Cell Adhesion Molecules Research
- Metastasis and carcinoma case studies
- Melanoma and MAPK Pathways
- Dark Matter and Cosmic Phenomena
- Monoclonal and Polyclonal Antibodies Research
- Gastroesophageal reflux and treatments
- Gastrointestinal disorders and treatments
- Cancer Research and Treatments
- Gastrointestinal Bleeding Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Gastrointestinal Tumor Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Celiac Disease Research and Management
Spitalul Clinic Colentina
2020
Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale"
2009-2018
Ospedale Antonio Cardarelli
2001-2014
Cancer Institute (WIA)
1989-2013
Università Cattolica del Sacro Cuore
2000-2012
University of Naples Federico II
2006-2011
University of Campania "Luigi Vanvitelli"
2006-2011
Tumour Institute of Tuscany
2009-2010
National Cancer Institute
2008
Istituto Nazionale di Fisica Nucleare, Sezione di Roma I
2001-2005
Abstract Stem cells, derived from human adult dental pulp of healthy subjects 30-45 years age, were cultured, and cells selected using a FACSorter. A new c-kit+/CD34+/CD45− cell population stromal bone producing (SBP/DPSCs) was selected, expanded, cultured. These SBP/DPSCs are highly clonogenic and, in culture, differentiate into osteoblast precursors (CD44+/RUNX-2+), still capable self-renewing, then osteoblasts, producing, vitro, living autologous fibrous (LAB) tissue, which is markedly...
Background Osteosarcoma is the most common primary tumour of bone. Solid tumours are made heterogeneous cell populations, which display different goals and roles in economy. A rather small subset can hold or acquire stem potentials, gaining aggressiveness increasing expectancy recurrence. The CD133 antigen a pentaspan membrane glycoprotein, has been proposed as cancer marker, since it previously demonstrated to be capable identifying initiating subpopulation brain, colon, melanoma other...
Abstract It is not known whether cells derived from stem retain their differentiation and morpho‐functional properties after long‐term cryopreservation. This information of importance to evaluate potential for storage with a view subsequent use in therapy. Here, we describe the dental pulp (SBP‐DPSCs), differentiated osteoblasts, recovered After 2 years, found that are still capable differentiation, cytotypes proliferate produce woven bone tissue. In addition, express all respective surface...
Abstract Stem cells were obtained from deciduous dental pulp of healthy subjects, aged 6–10 years. This stem cell population was cultured, expanded, and specifically selected, detecting using a FACsorter, c‐kit, CD34, STRO‐1 antigen expression. Then, c‐kit + /CD34 /STRO‐1 replaced in the culture medium added 20% FBS, leading to osteoblast differentiation. In fact, these cells, after week, showed large positivity for CD44, osteocalcin, RUNX‐2 markers. To achieve an adipocytic differentiation,...
Emerging evidence suggests that specific sub-populations of cancer cells with stem cell characteristics within the bulk tumours are implicated in pathogenesis heterogeneous malignant tumours. The drive tumour growth have been denoted cancer-initiating or (hereafter CSCs). CSCs isolated initially from leukaemias and subsequently several solid including brain, breast, prostate, colon lung cancer. This study aimed at isolating characterising population tumour-initiating non-small-cell...
This study aimed to identify, isolate, and characterize cancer stem cells from human primary sarcomas. We performed cytometric analyses for stemness differentiation antigens, including CD29, CD34, CD44, CD90, CD117, CD133, on 21 sarcomas the day of surgery. From sarcoma biopsies, we obtained 2 chondrosarcoma-stabilized cell lines osteosarcoma stabilized lines, which sphere formation, side population profile, gene expression, in vivo vitro assays were performed. All samples expressed CD29...
Somatic mutation (E17K) that constitutively activates the protein kinase AKT1 has been found in human cancer patients. We determined role of E17K lung cancer, through sequencing exon 4 105 resected, clinically annotated non-small cell specimens. detected a missense mutations G->A transition at nucleotide 49 (that results substitution) two squamous carcinoma (2/36) but not adenocarcinoma (0/53). The activity endogenous carrying immunoprecipitated by tumour tissue was significantly higher...
Background Human adult adipose tissue is an abundant source of mesenchymal stem cells (MSCs). Moreover, it easily accessible site producing a considerable amount cells. Methodology/Principal Findings In this study, we have selected and characterized within the stromal vascular fraction (SVF) human with aim understanding their differentiation capabilities performance. We found, SVF, different cell populations expressing MSC markers – including CD34, CD90, CD29, CD44, CD105, CD117...
Background Cancer Stem Cells (CSCs) hypothesis asserts that only a small subset of cells within tumour is capable both initiation and sustainment. The Epithelial-Mesenchymal Transition (EMT) an embryonic developmental program often activated during cancer invasion metastasis. aim this study to shed light on the relationship between EMT CSCs by using LC31 lung primary cell line. Materials Methods A549 lines were treated with 2 ng/ml TGFβ-1 for 30 days, 80 respectively. To evaluate EMT,...
TRAP-1 is a mitochondrial heat shock protein (HSP), recently identified in Saos-2 osteosarcoma cells adapted to mild oxidative stress induced by diethylmaleate (DEM). mRNA expression increased DEM-adapted as well tumor resistant 5-fluorouracil and platin derivatives. Since strong decrease of levels, upon cisplatin treatment, observed only controls but not the counterpart, possible role for this development phenotypes could be hypothesized. To characterize protective against apoptosis, stable...
Adult mesenchymal stem cells, such as dental pulp are of great interest for cell-based tissue engineering strategies because they can differentiate into a variety tissue-specific above all, osteoblasts. In recent years, epigenetic studies on cells have indicated that specific histone alterations and modifying enzymes play essential roles in cell differentiation. However, although several reported valproic acid (VPA)-a selective inhibitor deacetylases (HDAC)-enhances osteoblast...
Metastasis is the leading cause of death by cancer. Non-small-cell lung cancer (NSCLC) represents nearly 85% primary malignant tumours. Recent researches have demonstrated that epithelial-to-mesenchymal transition (EMT) plays a key role in early process metastasis cells. Transforming growth factor-β1 (TGF-β1) major inductor EMT. The aim this study to investigate TGF-β1’s effect on stem cells (CSCs) identified as positive for CD133, side population (SP) and non-cancer (non-CSCs) negative SP...
Aberrant activation of PI3K/AKT signalling represents one the most common molecular alterations in lung cancer, though relative contribution single components cascade to NSCLC development is still poorly defined. In this manuscript we have investigated relationship between expression and genetic pathway [KRAS, catalytic subunit PI3K (p110α), PTEN, AKT1 AKT2] AKT 107 surgically resected NSCLCs analyzed existing relationships with clinico-pathologic features. Expression analysis was performed...
CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem rich populations. Screening revealed that, ovarian OVCAR-3, -4 -5 colon HT-29, HCT-116 SW620 over expressed both proteins. We aimed isolate cells with features sorting expressing CXCR4(+)CD133(+) within lines. The sorted population CD133(+)CXCR4(+) demonstrated highest efficiency sphere formation OVCAR-4 OVCAR-5 cells. Moreover OCT4, SOX2, KLF4 NANOG highly Most strikingly formed number of tumors when inoculated...
Autologous graft is considered the gold standard of materials; however, this approach still limited due to both small amount tissue that can be collected and reduced cell viability cells obtained. The aim preliminary study was demonstrate efficacy an innovative medical device called Rigeneracons® (CE certified Class I) provide autologous micro‐grafts immediately available used in clinical practice. Moreover, instrument able create enriched progenitors which maintain their regenerative...
Background: One‐week low‐dose proton pump inhibitor‐based triple therapies have usually proved to be effective treatments for Helicobacter pylori infection. Aim: To investigate the eradication efficacy, safety profile and patient compliance of two containing a standard dose rabeprazole new fluoroquinolone, levofloxacin. Methods: One hundred patients referred us gastroscopy, who were H. ‐positive, consecutively recruited in prospective, open‐label study. The enrolled randomised receive...
Recent studies have shown potential ways for improving stem cell cryopreservation. The major need autologous use is a long-term storage: this arises from the humans' hope of future their own cells. Therefore, it important to evaluate vitality and differentiation before after Although several preservation adipose tissue, few them focused attention aim study was fate cryopreserved cells collected tissue stored at low temperature in liquid nitrogen through an optimal cryopreservation solution...
Dental pulp stem cells (DPSCs) are multipotent derived from neural crest and mesenchyme have the capacity to differentiate into multiple cell lineages.It has already been demonstrated that DPSCs melanocyte-like but only when cultivated in a specific melanocyte differentiating medium.In this study we shown, for first time, capable of spontaneously mature melanocytes, which display molecular ultrastructural features full development, including expression markers presence melanosomes up...