A5047667967- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Cancer-related gene regulation
- Autophagy in Disease and Therapy
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Electrospun Nanofibers in Biomedical Applications
- Sarcoma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Cancer Genomics and Diagnostics
- Bone health and treatments
- Lung Cancer Treatments and Mutations
- Cancer, Stress, Anesthesia, and Immune Response
- HER2/EGFR in Cancer Research
- Bone Tissue Engineering Materials
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- 3D Printing in Biomedical Research
- Axon Guidance and Neuronal Signaling
- Metabolism, Diabetes, and Cancer
- dental development and anomalies
- Pancreatic function and diabetes
University of Campania "Luigi Vanvitelli"
2016-2025
Azienda Ospedaliera Universitaria Università degli Studi della Campania Luigi Vanvitelli
2021-2025
University of Naples Federico II
2006-2017
University of Michigan
2013-2014
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2013-2014
Organogenesis (United States)
2013
Regione Campania
2009-2010
Temple University
2006-2007
Sbarro Health Research Organization
2007
Hahnemann University Hospital
1959
In this study we used a biocomplex constructed from dental pulp stem/progenitor cells (DPCs) and collagen sponge scaffold for oro-maxillo-facial (OMF) bone tissue repair in patients requiring extraction of their third molars.The experiments were carried out according to our Internal Ethical Committee Guidelines written informed consent was obtained the patients.The presented with bilateral reabsorption alveolar ridge distal second molar secondary impaction on cortical lamina, producing...
Background Osteosarcoma is the most common primary tumour of bone. Solid tumours are made heterogeneous cell populations, which display different goals and roles in economy. A rather small subset can hold or acquire stem potentials, gaining aggressiveness increasing expectancy recurrence. The CD133 antigen a pentaspan membrane glycoprotein, has been proposed as cancer marker, since it previously demonstrated to be capable identifying initiating subpopulation brain, colon, melanoma other...
Abstract Pentose phosphate pathway (PPP) is a major glucose metabolism pathway, which has fundamental role in cancer growth and metastasis. Even though PPP blockade been pointed out as very promising strategy against cancer, effective anti-PPP agents are not still available the clinical setting. Here we demonstrate that natural molecule polydatin inhibits glucose-6-phosphate dehydrogenase (G6PD), key enzyme of PPP. Polydatin blocks G6PD causing accumulation reactive oxygen species strong...
Abstract Background Breast cancer (BC) is a complex disease, showing heterogeneity in the genetic background, molecular subtype, and treatment algorithm. Historically, strategies have been directed towards cells, but these are not unique components of tumor bulk, where key role played by microenvironment (TME), whose better understanding could be crucial to obtain outcomes. Methods We evaluated mitochondrial transfer (MT) co-culturing Adipose stem cells with different (BCCs), through...
This study aimed to identify, isolate, and characterize cancer stem cells from human primary sarcomas. We performed cytometric analyses for stemness differentiation antigens, including CD29, CD34, CD44, CD90, CD117, CD133, on 21 sarcomas the day of surgery. From sarcoma biopsies, we obtained 2 chondrosarcoma-stabilized cell lines osteosarcoma stabilized lines, which sphere formation, side population profile, gene expression, in vivo vitro assays were performed. All samples expressed CD29...
Abstract Mesenchymal stem cells deriving from dental pulp differentiate into osteoblasts capable of producing bone. In previous studies, we extensively demonstrated that, when seeded on collagen I scaffolds, these can be conveniently used for the repair human mandible defects. Here, assess stability and quality regenerated bone vessel network 3 years after grafting intervention, with conventional procedures in-line holotomography, an advanced phase-imaging method using synchrotron radiation...
Background Human adult adipose tissue is an abundant source of mesenchymal stem cells (MSCs). Moreover, it easily accessible site producing a considerable amount cells. Methodology/Principal Findings In this study, we have selected and characterized within the stromal vascular fraction (SVF) human with aim understanding their differentiation capabilities performance. We found, SVF, different cell populations expressing MSC markers – including CD34, CD90, CD29, CD44, CD105, CD117...
Adult mesenchymal stem cells, such as dental pulp are of great interest for cell-based tissue engineering strategies because they can differentiate into a variety tissue-specific above all, osteoblasts. In recent years, epigenetic studies on cells have indicated that specific histone alterations and modifying enzymes play essential roles in cell differentiation. However, although several reported valproic acid (VPA)-a selective inhibitor deacetylases (HDAC)-enhances osteoblast...
Glucose-6-phospate dehydrogenase (G6PD) is the limiting enzyme of pentose phosphate pathway (PPP) correlated to cancer progression and drug resistance. We previously showed that G6PD inhibition leads Endoplasmic Reticulum (ER) stress often associated autophagy deregulation. The latter can be induced by target-based agents such as Lapatinib, an anti-HER2 tyrosine kinase inhibitor (TKI) largely used in breast treatment. Here we investigate whether causes alteration, which potentiate Lapatinib...
The long non-coding RNA (lncRNA), MALAT1, plays a key role in the development of different cancers, and its expression is associated with worse prognosis patients. However, mechanism action regulation are not well known prostate cancer (PCa). A general lncRNAs their interaction other epigenetic regulators including microRNAs (miRNAs).Using lentiviral stable miRNA transfection together cell biology functional assays gene expression/target analysis, we investigated between MALAT1 miR-423-5p,...
Recent studies have shown potential ways for improving stem cell cryopreservation. The major need autologous use is a long-term storage: this arises from the humans' hope of future their own cells. Therefore, it important to evaluate vitality and differentiation before after Although several preservation adipose tissue, few them focused attention aim study was fate cryopreserved cells collected tissue stored at low temperature in liquid nitrogen through an optimal cryopreservation solution...
Human dental pulp stem cells (hDPSCs) are mesenchymal that have been successfully used in human bone tissue engineering. To establish whether these can lead to a ready be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining new tool transplantation. Therefore, hDPSCs were specifically selected from stromal-vascular fraction, using appropriate markers, cultured. Growth curves, expression bone-related calcification...
// Vincenzo Desiderio 1,3,* , Petros Papagerakis 4,5,* Virginia Tirino 3,* Li Zheng 2 Margarite Matossian 1 Mark E. Prince 6 Francesca Paino 3 Luigi Mele 1,3 Federica Papaccio 3, Roberta Montella Gianpaolo 3,** and Silvana 1,2,** Department of Otolaryngology, Medical School, University Michigan, Ann Arbor, MI, USA Periodontics Oral Medicine, School Dentistry, Experimental Section Biotechnology Histology Embryology, Second Naples, Italy 4 Orthodontics Pediatric 5 Center for Organogenesis,...
The EPHA2 tyrosine kinase receptor is implicated in tumor progression and targeted therapies resistance. We evaluated as a potential resistance marker to the antiepidermal growth factor (EGFR) monoclonal antibody cetuximab colorectal cancer. studied activation of panel human cancer cell lines sensitive or resistant anti-EGFR drugs. vitro vivo effects ALW-II-41-27 (an inhibitor) and/or treatment were tested. Formalin-fixed paraffin-embedded specimens from 82 RAS wild-type (WT) metastatic...
Recent studies showed that mesenchymal stem cells derived from adipose tissue can promote tumour progression, raising some concerns regarding their use in regenerative medicine. In this context, we co-cultured either SAOS2 osteosarcoma or MCF7 breast cancer with human (hASCs), order to evaluate potential effects of on hASCs differentiation, vitro and vivo. study observed both cell lines induced an increase proliferation, compared alone, but, surprisingly, neither changes the expression CD90,...
Cancer stem cells (CSCs) play a key role in cancer initiation, progression and chemoresistance. Epigenetic alterations have been identified as prominent factors that contribute to the CSCs phenotype. Here, we investigated effects of HDAC inhibitor valproic acid (VPA) demethylating agent, 5'azacytidine (DAC) on phenotype MG63 Saos2 osteosarcoma cell lines. were treated with DAC VPA, alone combination. Untreated examined for stemness by cytometry real-time PCR. Sarcospheres colonies formation...
In the tumor microenvironment, cancer cells experience hypoxia resulting in accumulation of misfolded/unfolded proteins largely endoplasmic reticulum (ER). Consequently, ER proteotoxicity elicits unfolded protein response (UPR) as an adaptive mechanism to resolve stress. addition canonical UPR, also stimulates selective, autophagy-dependent, removal discrete domains loaded with misfolded further alleviate These mechanisms can favor cell growth, metastasis, and long-term survival. Our...
Stem cell based therapies for the repair and regeneration of various tissues are great interest a high number diseases. Adult stem cells, instead, more available, abundant harvested with minimally invasive procedures. In particular, mesenchymal cells (MSCs) multi-potent progenitors, able to differentiate into bone, cartilage, adipose tissues. Human adult tissue seems be most source MSCs and, due its easy accessibility; it is give considerable amount cells. this study, we selected...