A5002232333- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Cellular Mechanics and Interactions
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- Cancer Genomics and Diagnostics
Institute of Genetics and Biophysics
2022-2024
Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is the most lethal cancer with an aggressive metastatic phenotype and very poor clinical prognosis. Interestingly, a lower occurrence of PDAC has been described in individuals severe long-standing asthma. Here we explored potential link between glucocorticoid (GC) budesonide, first-line therapy to treat Methods We tested effect budesonide classical GCs on morphology, proliferation, migration invasiveness patient-derived cells...
3D embryonic stem cell (ESC) aggregates self-organize into embryo-like structures named gastruloids that recapitulate the axial organization of post-implantation embryos. Crucial in this process is symmetry-breaking event leads to emergence asymmetry and spatially ordered from homogeneous aggregates. Here, we show budesonide, a glucocorticoid drug widely used treat asthma, prevents ESC break symmetry. Mechanistically, effect budesonide receptor independent. RNA sequencing lineage fate...
Small molecules that can modulate or stabilize cell–cell interactions are valuable tools for investigating the impact of collective cell behavior on various biological processes such as development/morphogenesis, tissue regeneration and cancer progression. Recently, we showed budesonide, a glucocorticoid widely used an anti-asthmatic drug, is potent regulator stem pluripotency. Here tested effect different budesonide derivatives identified CHD-030498 more effective analogue budesonide. was...
The role and the mechanisms of long non-coding RNA (lncRNAs) in early mammalian embryogenesis remain unclear; this is mostly due to complexity regulatory mechanism lncRNAs limited availability embryo samples. emergence stem cell-based models offers new opportunities address these challenging questions. Here we use mouse gastruloids investigate an ultraconserved lncRNA, T-UCstem1, formation body plan. Combining morphological immunofluorescence analysis with bulk single cells transcriptomics,...
Small molecules that can modulate or stabilize cell-cell interactions are valuable tools for investigating the impact of collective cell behavior on various biological processes such as development/morphogenesis, tissue regeneration and cancer progression. Recently, we showed budesonide, a glucocorticoid widely used anti-asthmatic drug, is potent regulator stem pluripotency. Here tested effect different budesonide derivatives identified CHD-030498 more effective analogue budesonide. was able...