A5087735778- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Nanoparticles: synthesis and applications
- Plant-Microbe Interactions and Immunity
- Bacterial biofilms and quorum sensing
- Enzyme-mediated dye degradation
- Bacterial Infections and Vaccines
- Bacterial Genetics and Biotechnology
- Celiac Disease Research and Management
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Biocrusts and Microbial Ecology
- Gut microbiota and health
- Insect symbiosis and bacterial influences
- Probiotics and Fermented Foods
- Microscopic Colitis
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Bone Tissue Engineering Materials
- Helicobacter pylori-related gastroenterology studies
- Connective tissue disorders research
- Electrospun Nanofibers in Biomedical Applications
- Legume Nitrogen Fixing Symbiosis
- Escherichia coli research studies
Czech Academy of Sciences
2013-2024
Czech Academy of Sciences, Institute of Microbiology
2014-2024
Laboratory of Molecular Genetics
2013
Character
2013
Palacký University Olomouc
2010
Massey University
2010
Charles University
1997
The aim was to analyze the influence of intestinal microbiota on development inflammation. We used model chronic inflammation that develops spontaneously in colon conventional severe combined immunodeficiency (SCID) mice restored with CD45 RB(high) subset CD4+T cells isolated from spleen normal BALB/c mice.A CD4+CD45RB(high) subpopulation T purified by magnetic separation (MACS) and transferred into immunodeficient SCID mice. Germ-free (GF) or monoassociated Enterococcus faecalis, SFB...
Background and Aims Celiac disease (CD) is a chronic inflammatory disorder of the small intestine that induced by dietary wheat gluten proteins (gliadins) in genetically predisposed individuals. The overgrowth potentially pathogenic bacteria infections has been suggested to contribute CD pathogenesis. We aimed study effects gliadin various intestinal bacterial strains on mucosal barrier integrity, translocation, cytokine production. Methodology/Principal Findings Changes gut mucosa were...
ABSTRACT How bacteria control proper septum placement at midcell, to guarantee the generation of identical daughter cells, is still largely unknown. Although different systems involved in selection division site have been described selected species, these do not appear be widely conserved. Here, we report that LocZ (Spr0334), a newly identified cell protein, Streptococcus pneumoniae . We show locZ essential but its deletion results defects and shape deformation, causing cells divide...
Adenylate cyclase toxin (CyaA or ACT) is a key virulence factor of pathogenic Bordetellae. It penetrates phagocytes expressing the alpha(M)beta(2) integrin (CD11b/CD18, Mac-1 CR3) and paralyzes their bactericidal capacities by uncontrolled conversion ATP into signaling molecule, cAMP. Using pull-down activity assays transfections with mutant Rho family GTPases, we show that cAMP CyaA causes transient selective inactivation RhoA in mouse macrophages absence detectable activation Rac1, Rac2,...
Spore awakening is a series of actions that starts with purely physical processes and continues via the launching gene expression metabolic activities, eventually achieving vegetative phase growth. In spore-forming microorganisms, germination process controlled by intra- inter-species communication. However, in Streptomyces clade, which capable developing plethora valuable compounds, chemical signals produced during have not been systematically studied before. Our previously published data...
Reversible protein phosphorylation catalyzed by kinases and phosphatases is the primary mechanism for signal transduction in all living organisms. Streptococcus pneumoniae encodes a single Ser/Thr kinase, StkP, which plays role virulence, stress resistance regulation of cell wall synthesis division. However, its cognate phosphatase, PhpP, not well defined.Here, we report successful construction ΔphpP mutant unencapsulated S. Rx1 strain characterization phenotype. We demonstrate that PhpP...
Titanium dioxide nanoparticles (TiO2NPs) are revolutionizing biomedicine due to their potential application as diagnostic and therapeutic agents. However, the TiO2NP immune-compatibility remains an open issue, even for ethical reasons. In this work, we investigated immunomodulatory effects of TiO2NPs in emergent proxy human non-mammalian model vitro basic translational immunology: sea urchin Paracentrotus lividus. To highlight on new insights into evolutionarily conserved intracellular...
Abstract Bacterial nanotubes are membranous structures that have been reported to function as conduits between cells exchange DNA, proteins, and nutrients. Here, we investigate the morphology formation of bacterial using Bacillus subtilis . We show nanotube is associated with stress conditions, highly sensitive cells’ genetic background, growth phase, sample preparation methods. Remarkably, appear be extruded exclusively from dying cells, likely a result biophysical forces. Their emergence...
AbstractŠkaloud P., Kynčlová A., Benada O., Kofroňová O. and Škaloudová M. 2012. Toward a revision of the genus Synura, section Petersenianae (Synurophyceae, Heterokontophyta): morphological characterization six pseudo-cryptic species. Phycologia 51: 303–329. DOI: 10.2216/11-20.1Morphological data, based on transmission scanning electron microscopy silica scales, are provided for genetic lineages Synura petersenii species complex as revealed by multiple markers (internal transcribed spacer...
Francisella tularensis is a Gram-negative, facultative intracellular bacterium, causing severe disease called tularemia. It secretes unusually shaped nanotubular outer membrane vesicles (OMV) loaded with number of virulence factors and immunoreactive proteins. In the present study were purified from clinical isolate subsp. holarctica strain FSC200. We here provide comprehensive proteomic characterization OMV using novel approach in which comparison fraction performed order to find proteins...
Protein turnover is essential in all living organisms for the maintenance of normal cell physiology. In eukaryotes, most cellular protein involves ubiquitin-proteasome pathway, which proteins tagged with ubiquitin are targeted to proteasome degradation. contrast, bacteria lack a but harbor proteases turnover. However, some actinobacteria, such as mycobacteria, possess addition these proteases. A prokaryotic ubiquitination-like tagging process mycobacteria was described and named pupylation:...
Cell infiltration is a critical parameter for the successful development of 3D matrices tissue engineering. Application electrospun nanofibers in engineering has recently attracted much attention. Notwithstanding several their advantages, small pore size and thickness layer limit application scaffolds. Several methods and/or increase have been developed. Nevertheless, still needs emerging either novel nanofiber-enriched composites or new techniques nanofiber fabrication. Forcespinning(®)...
Extensive exploitation of titanium dioxide nanoparticles (TiO2NPs) augments rapid release into the marine environment. When in contact with body fluids invertebrates, TiO2NPs undergo a transformation and adhere various organic molecules that shape complex protein corona prior to contacting cells tissues. To elucidate potential extracellular signals may be involved particle recognition by immune sea urchin Paracentrotus lividus, we investigated behaviour proteins vitro. Our findings indicate...
Macrophages are key sentinel cells of the immune system, and, as such, they targeted by toxins produced pertussis agent Bordetella . The adenylate cyclase toxin (CyaA) mediates evasion B. suspending bactericidal activities myeloid phagocytes. We reveal a novel mechanism potential subversion host immunity, where CyaA at very low (22 pM) concentrations could inhibit maturation human monocyte precursors into more phagocytic macrophage cells. Furthermore, exposure to amounts has been shown...
A novel bacterial strain, designated M8T, was isolated from milk of a female macaque bred in captivity. The strain Gram-stain-positive, anaerobic, irregular coccoid–rod-shaped without catalase activity. Analysis 16S rRNA gene sequence similarity revealed that the isolate most closely related to Alloscardovia omnicolens CCUG 31649T (96.4 %) and Metascardovia criceti OMB105T (96.6 %). Sequences hsp60, fusA, xfp genes also confirmed type strains A. M. . produced fructose-6-phosphate...
Pseudallescheria boydii sensu lato is an emerging fungal pathogen causing fatal infections in both immunocompromised and immunocompetent hosts. In this work, two P. isolates (human animal origin) have been identified as being producers of cyclic peptides. Five putative nonribosomal peptides with a unique structure, which named pseudacyclins, were characterized by nuclear magnetic resonance spectroscopy mass spectrometry. The most abundant representative the pseudacyclins was quantified also...
Three unknown Gram-stain-positive, catalase-negative, facultatively anaerobic and coccus-shaped strains of bacteria were isolated from the digestive tracts wasps (Vespula vulgaris). Analysis 16S rRNA gene sequences revealed that these had identical showed Vagococcus salmoninarum, with 96.2% sequence similarity, was closest phylogenetic neighbour. Further analyses based on hsp60 pheS representatives family Enteroccocaceae genotypic phenotypic characterization using (GTG)5-PCR fingerprintings,...