A5044827296- Gut microbiota and health
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Ruminant Nutrition and Digestive Physiology
- Clostridium difficile and Clostridium perfringens research
- Biofuel production and bioconversion
- Dermatology and Skin Diseases
- Plant and fungal interactions
- Nonmelanoma Skin Cancer Studies
- Cutaneous Melanoma Detection and Management
- Microbial Metabolites in Food Biotechnology
- Hepatitis C virus research
- Microbial infections and disease research
- Bacterial Identification and Susceptibility Testing
- Insect and Pesticide Research
- Oral microbiology and periodontitis research
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Enzyme Production and Characterization
- Plant Pathogens and Fungal Diseases
- Liver Disease Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Insect Utilization and Effects
- Mycobacterium research and diagnosis
- Gastrointestinal motility and disorders
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2015-2025
University of Georgia
2009-2024
Czech Academy of Sciences
2001-2023
Institute of Animal Physiology of the Slovak Academy of Sciences
2012-2022
Public Health Institute Ostrava
2021
Czech Academy of Sciences, Institute of Microbiology
2015
Charles University
2011
A 1000-cow study across four European countries was undertaken to understand what extent ruminant microbiomes can be controlled by the host animal and identify characteristics of rumen microbiome axis that determine productivity methane emissions. core microbiome, phylogenetically linked with a preserved hierarchical structure, identified. 39-member subset formed hubs in co-occurrence networks linking structure genetics phenotype (methane emissions, blood metabolites, milk production...
Summary Commensal bacteria have been shown to modulate the host mucosal immune system. Here, we report that oral treatment of BALB/c mice with components from commensal, Parabacteroides distasonis, significantly reduces severity intestinal inflammation in murine models acute and chronic colitis induced by dextran sulphate sodium (DSS). The membranous fraction P. distasonis (mPd) prevented DSS-induced increases several proinflammatory cytokines, increased mPd-specific serum antibodies...
Background Probiotic bacteria can be used for the prevention and treatment of human inflammatory diseases including bowel (IBD). However, nature active components exact mechanisms this beneficial effects have not been fully elucidated. Our aim was to investigate if lysate probiotic bacterium L. casei DN-114 001 (Lc) could decrease severity intestinal inflammation in a murine model IBD. Methodology/Principal Findings The preventive effect oral administration Lc significantly reduces acute...
Two novel Gram-negative, anaerobic, non-spore-forming, butyrate-producing bacterial species, strains Mz 5T and JK 615T, were isolated from the rumen fluid of cow sheep. Both curved rods that motile by means single polar or subpolar flagellum common in microbial ecosystem. Strain produced high xylanase, proteinase, pectin hydrolase DNase activities; 1,4-β-endoglucanase was also detected culture medium. The bacterium utilized a wide range carbohydrates. Glucose fermented to formate, butyrate,...
The metabolic activities of gut microbes significantly influence host physiology; thus, characterizing the forces that modulate this micro-ecosystem is key to understanding mammalian biology and fitness. To investigate microbiome wild primates determine how these microbial communities respond host's external environment, we characterized faecal bacterial and, for first time, metabolomes four lowland gorilla groups in Dzanga-Sangha Protected Areas, Central African Republic. Results show...
Social honey bees, Apis mellifera, host a set of distinct microbiota, which is similar across the continents and various bee species. Some these bacteria, such as lactobacilli, have been linked to immunity defence against pathogens. Pathogen crucial, particularly in larval stages, many pathogens affect brood. However, information on microbiota conflicting. Seven developmental stages drones were sampled from 3 colonies at each 4 geographic locations A. mellifera carnica, samples maintained...
Abstract Background The microbiome alterations are associated with cancer growth and may influence the immune system response to therapy. Particularly, gut has been recently shown modulate melanoma immunotherapy. However, role of skin not well explored in tumour microenvironment link between investigated progression. Therefore, aim present study was examine associations dysbiosis using MeLiM porcine model progression spontaneous regression. Results Parallel analysis cutaneous microbiota...
Three strains of a butyrate-producing bacterium were isolated from the rumen contents grazing sheep and cows. The anaerobic, with Gram-positive cell walls, straight-to-slightly-curved, rod-shaped, non-spore-forming single flagellate. C14 : 1, 0, C16 0 1 predominant fatty acids. cell-wall peptidoglycan type was A1γ. DNA G+C content varied 41.4 to 42.2 mol%. 16S rRNA gene sequence similarities between isolates Eubacterium rectale, Roseburia hominis intestinalis found be 96, 95 %, respectively....
Microbial sensing by Toll-like receptors (TLR) and its negative regulation have an important role in the pathogenesis of inflammation-related cancer. In this study, we investigated signaling gut microbiota development colitis-associated cancer mouse model.Colitis-associated was induced azoxymethane dextran sodium sulfate wild-type interleukin-1 receptor-associated kinase M (IRAK-M)-deficient mice with or without antibiotic (ATB) treatment. Local cytokine production analyzed multiplex assay...
Abstract Cutaneous leishmaniasis, a parasitic disease caused by Leishmania major , is widely frequent form in humans. To explore the importance of host gut microbiota and to investigate its changes during L. infection, two different groups mouse models were assessed. The microbiome parts gut—ileum colon—from infected non-infected mice characterised sequencing 16S rDNA using an Ion Torrent PGM platform. Microbiome analysis was performed reveal related susceptibility genetics strains...
Our previous study, based primarily on PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing, focused the isolation of four bifidobacterial groups from digestive tract three bumblebee species. In that we proposed these isolated potentially represented novel species family Bifidobacteriaceae. One four, Bifidobacterium bombi, has been described recently. Strains representing two other have classified as members genus basis positive results for fructose-6-phosphate...
Gram-positive-staining, anaerobic, non-spore-forming, lactate- and acetate-producing bacterial strains were isolated from the digestive tracts of different bumblebee species (Bombus lucorum, Bombus pascuorum lapidarius). All isolates produced fructose-6-phosphate phosphoketolase activity. A representative strain, BluCI/TPT, was characterized further. Cells strain BluCI/TPT showed occasional bifurcation irregular constrictions. The bacterium utilized a wide range carbohydrates. Glucose...
Summary The aim was to assess the effects of intact dried Ascophyllum nodosum seaweed on piglet performances, gut bacteria and function plasma oxidative status. A total 160 weaned piglets (21 days, 6.59 ± 0.91 kg) were allocated four dietary treatments with eight pen replicates five animals each for 28 days: a control diet; based cereals, soybean meal milk products, three basal diets supplemented either 2.5, 5.0 or 10.0 g per kg. At day 12/13 one from sacrificed. Plasma samples taken...
Ulcerative colitis (UC) is an inflammatory bowel disease, and intestinal bacteria are implicated in the pathogenesis of this disorder. The administration aminosalicylates (5-ASA) a conventional treatment that targets mucosa, while fecal microbial transplantation (FMT) novel directly gut microbiota. aim study was to identify changes bacterial composition after both types treatments evaluate clinical responses. Sixteen patients with active left-sided UC underwent enema using 5-ASA (n = 8) or...
Background and Aims: Ulcerative colitis (UC) is a chronic inflammatory disease. Fecal microbial transplantation (FMT) promising alternative treatment. Methods: This multicenter, open-label, noninferiority trial randomized patients with active left-sided UC (Mayo score 4–10) equally to FMT or 5-aminosalicylic acid (5-ASA) enemas. enemas were administered five times in the first week then once weekly for 5 weeks. 5-ASA daily 2 weeks every other day. The primary study endpoint was clinical...
Anaerobic fungi ( AF ) are able to degrade crop substrates with higher efficiency than commonly used anaerobic bacteria. The aim of this study was investigate ways use rumen improve biogas production from energy crops under laboratory conditions. In study, strains isolated feces or fluid cows and deer were tested for their ability integrate into the bacterial ecosystem production, in order degradation substrate polysaccharides consequently yield. Batch culture, fed batch semicontinuous...
Background: Disturbances in the intestinal microbial community (i.e.dysbiosis) or presence of microbes with deleterious effects on colonic mucosa has been linked to pathogenesis inflammatory bowel diseases.However role microbiota induction and progression ulcerative colitis (UC) not yet fully elucidated.Methods: Three lines human microbiota-associated (HMA) mice were established by gavage colon biopsy from three patients active UC.The shift during its transferring humans was analyzed...