A5012819872- Gut microbiota and health
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Asthma and respiratory diseases
- Dermatology and Skin Diseases
- Food Allergy and Anaphylaxis Research
- Infant Nutrition and Health
- Pediatric health and respiratory diseases
- Allergic Rhinitis and Sensitization
- Escherichia coli research studies
- Stress Responses and Cortisol
- Glycosylation and Glycoproteins Research
- Drug Transport and Resistance Mechanisms
- Inflammatory Bowel Disease
- Microbial Metabolites in Food Biotechnology
- Immune Response and Inflammation
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Neuroendocrine regulation and behavior
- Digestive system and related health
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Insect behavior and control techniques
- Insect symbiosis and bacterial influences
- Ginseng Biological Effects and Applications
Czech Academy of Sciences, Institute of Microbiology
2016-2025
Czech Academy of Sciences
2012-2024
Rockefeller University
2023
Toxalim Research Centre in Food Toxicology
2014
Université Toulouse III - Paul Sabatier
2014
Roslin Institute
2014
German Cancer Research Center
2014
Heidelberg University
2014
Alder Hey Children's Hospital
2014
University of Liverpool
2014
In most animal species, juvenile growth is marked by an exponential gain in body weight and size. Here we show that the microbiota of infant mice sustains both longitudinal when are fed a standard laboratory mouse diet or nutritionally depleted diet. We found intestinal interacts with somatotropic hormone axis to drive systemic growth. Using monocolonized models, showed selected lactobacilli promoted strain-dependent manner recapitulated microbiota's effect on axis. These findings host's...
The intestinal microbiota is known to influence postnatal growth. We previously found that a strain of Lactiplantibacillus plantarum (strain Lp WJL ) buffers the adverse effects chronic undernutrition on growth juvenile germ-free mice. Here, we report sustains malnourished conventional animals and supports both insulin-like factor–1 (IGF-1) insulin production activity. have identified cell walls isolated from , as well muramyl dipeptide mifamurtide, sufficient cues stimulate animal despite...
Background Probiotic bacteria can be used for the prevention and treatment of human inflammatory diseases including bowel (IBD). However, nature active components exact mechanisms this beneficial effects have not been fully elucidated. Our aim was to investigate if lysate probiotic bacterium L. casei DN-114 001 (Lc) could decrease severity intestinal inflammation in a murine model IBD. Methodology/Principal Findings The preventive effect oral administration Lc significantly reduces acute...
Background Reduced microbial diversity has been associated with inflammatory bowel disease (IBD) and probiotic bacteria have proposed for its prevention and/or treatment. Nevertheless, comparative studies of strains the same subspecies specific health benefits are scarce. Here we compared two Bifidobacterium longum ssp. their capacity to prevent experimental colitis. Methods Immunomodulatory properties nine bifidobacteria were assessed by stimulation murine splenocytes. The immune responses...
A decrease in the abundance and biodiversity of intestinal bacteria within Firmicutes phylum has been associated with inflammatory bowel disease (IBD). In particular, anti-inflammatory bacterium Faecalibacterium prausnitzii, member one most abundant species healthy human colon, is underrepresented microbiota IBD patients. The aim this study was to investigate immunomodulatory properties F. prausnitzii strain A2-165, biofilm forming HTF-F extracellular polymeric matrix (EPM) isolated from...
The Oligo-Mouse-Microbiota (OMM12) is a recently developed synthetic bacterial community for functional microbiome research in mouse models (Brugiroux et al., 2016). To date, the OMM12 model has been established several germ-free facilities world-wide and employed to address growing variety of questions related infection biology, mucosal immunology, microbial ecology host-microbiome metabolic cross-talk. consists 12 sequenced publically available strains isolated from mice, representing five...
The gut microbiota play an important role in shaping brain functions and behavior, including the activity of hypothalamus-pituitary-adrenocortical (HPA) axis. However, little is known about effect on distinct structures (hypothalamus, pituitary, adrenals) HPA In present study, we analyzed influence acute restraint stress (ARS) response adrenal gland intestine, organ extra-adrenal glucocorticoid synthesis. Using specific pathogen-free (SPF) germ-free (GF) male BALB/c mice, showed that plasma...
Background: Mucosal mast cells (MC) are key players in IgE-mediated food allergy (FA). The evidence on the interaction between gut microbiota, MC and susceptibility to FA is contradictory. Objective: We tested hypothesis that commensal bacteria essential for migration their maturation impacting FA. Methods: development severity of symptoms was studied sensitized germ-free (GF), conventional (CV), mice mono-colonized with L. plantarum WCFS1 or co-housed CV mice. were phenotypically...
Psoriatic patients have altered microbiota, both in the intestine and on skin. It is not clear, however, whether this a cause or consequence of disease. In study, using an experimental mouse model psoriasis induced by imiquimod (IMQ), we show that oral treatment with broad spectrum antibiotics (MIX) metronidazole (MET) alone mitigates severity skin inflammation through downregulation Th17 immune response conventional mice. Since some antibiotics, including MET, can influence system...
Abstract Mus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, undefined composition interindividual diversity of microbiota laboratory mice remains a limitation. In an attempt gut microbiome in preclinical mouse studies, here we report development simplified composed 15 strains from 7 20 most prevalent bacterial families representative fecal C57BL/6J Specific (and Opportunistic) Pathogen-Free...
Intestinal epithelial cells have the capacity to upregulate MHCII molecules in response certain epithelial-adhesive microbes, such as segmented filamentous bacteria (SFB). However, mechanism regulating expression well impact of MHCII-mediated antigen presentation on T cell responses targeting those microbes remains elusive. Here, we identify cellular network that regulates intestinal epithelium SFB. Since is dispensable for SFB-induced Th17 response, explored other CD4+ cell-based induced by...
Summary One of the promising approaches in therapy ulcerative colitis is administration butyrate, an energy source for colonocytes, into lumen colon. This study investigates effect butyrate producing bacterium Clostridium tyrobutyricum on dextran sodium sulphate (DSS)-induced mice. Immunocompetent BALB/c and immunodeficient severe combined immunodeficiency (SCID) mice reared specific-pathogen-free (SPF) conditions were treated intrarectally with C. 1 week prior to induction DSS during oral...
The egg protein ovalbumin (OVA) belongs to six most frequent food allergens. We investigated how thermal processing influences its ability induce allergic symptoms and immune responses in mouse model of allergy.Effect increased temperature (70°C 95°C) on OVA secondary structure was characterized by circular dichroism the kinetics pepsin digestion with subsequent HPLC. BALB/c mice were sensitized intraperitoneally challenged repeated gavages or heated 70°C (h-OVA). Levels allergen-specific...
Background: Disturbances in the intestinal microbial community (i.e.dysbiosis) or presence of microbes with deleterious effects on colonic mucosa has been linked to pathogenesis inflammatory bowel diseases.However role microbiota induction and progression ulcerative colitis (UC) not yet fully elucidated.Methods: Three lines human microbiota-associated (HMA) mice were established by gavage colon biopsy from three patients active UC.The shift during its transferring humans was analyzed...
ABSTRACT The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results stimulation mouse bone marrow-derived dendritic cells (BM-DC) human embryonal kidney (HEK293) stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. produces PS that differ greatly in their structure. polymer L900/2, a high average molecular mass 830 kDa, is branched heteropolysaccharide unique repeating unit consisting seven sugar residues...
Epidermal growth factor receptor (EGFR)-targeted anticancer therapy induces stigmatizing skin toxicities affecting patients' quality of life and adherence. The lack mechanistic details underlying these adverse events hampers their management. We found that EGFR/ERK signaling is required in LRIG1-positive stem cells during de novo hair eruption to secure barrier integrity prevent the invasion commensal microbiota inflammatory disease. EGFR-deficient epidermis permissive for outgrowth displays...
The Lactobacillus casei strain, LOCK 0919, is intended for the dietary management of food allergies and atopic dermatitis (LATOPIC® BIOMED). use a probiotic to modulate immune responses an interesting strategy solving imbalance problems gut microflora that may lead various disorders. However, exact bacterial signaling mechanisms underlying such modulations are still far from being understood. Here, we investigated variations in chemical compositions immunomodulatory properties...
Glucocorticoids (GCs) are hormones that released in response to stressors and exhibit many activities, including immunomodulatory anti-inflammatory activities. They primarily synthesized the adrenal gland but also produced peripheral tissues via regeneration of 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated influence microbiota on steroidogenesis corticosterone intestine germ-free (GF) specific pathogen-free mice challenged with a physical...
Interleukin (IL)-17 protects epithelial barriers by inducing the secretion of antimicrobial peptides. However, effect IL-17 on Paneth cells (PCs), major producers peptides in small intestine, is unclear. Here, we show that targeted ablation receptor (IL-17R) PCs disrupts their functions and decreases frequency ileal PCs. These changes become more pronounced after colonization with segmented filamentous bacteria. Mice lack IL-17R an increased inflammatory transcriptional profile ileum along...
Microbiota plays a role in shaping the HPA-axis response to psychological stressors. To examine of microbiota acute immune stressor, we stimulated adaptive system by anti-CD3 antibody injection and investigated expression adrenal steroidogenic enzymes profiling plasma corticosteroids their metabolites specific pathogen-free (SPF) germ-free (GF) mice. Using UHPLC-MS/MS, showed that 4 hours after challenge levels pregnenolone, progesterone, 11-deoxycorticosterone, corticosterone (CORT),...