A5021356344- Gut microbiota and health
- Inflammatory Bowel Disease
- Mycobacterium research and diagnosis
- Psoriasis: Treatment and Pathogenesis
- Microscopic Colitis
- Dermatology and Skin Diseases
- Genetic factors in colorectal cancer
- Digestive system and related health
- Cancer Immunotherapy and Biomarkers
- Viral-associated cancers and disorders
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Complementary and Alternative Medicine Studies
- Lymphoma Diagnosis and Treatment
- Gestational Diabetes Research and Management
- Lipid metabolism and disorders
- Celiac Disease Research and Management
- Helicobacter pylori-related gastroenterology studies
- Glycosylation and Glycoproteins Research
- Nail Diseases and Treatments
- RNA modifications and cancer
- Liver Diseases and Immunity
- Autoimmune and Inflammatory Disorders
- Probiotics and Fermented Foods
- Immune Response and Inflammation
Czech Academy of Sciences, Institute of Microbiology
2019-2025
Czech Academy of Sciences
2019-2025
Charles University
2022
Iscare I.V.F
2022
Czech Academy of Sciences, Institute of Molecular Genetics
2019
Diet is a major factor determining gut microbiota composition and perturbances in this complex ecosystem are associated with the inflammatory bowel disease (IBD). Here, we used gnotobiotic approach to analyze, how interaction between diet rich proteins influences sensitivity intestinal inflammation murine model of ulcerative colitis. We found that animal protein (aHPD) exacerbates acute dextran sulfate sodium (DSS)-induced colitis while plant (pHPD) does not. The deleterious effect aHPD was...
Psoriasis is a chronic inflammatory skin disease, whose pathogenesis involves dysregulated interplay among immune cells, keratinocytes and environmental triggers, including microbiota. Bacterial fungal dysbiosis has been recently associated with several immune-mediated diseases psoriasis. In this comprehensive study, we investigated how different sampling sites methods reflect the uncovered microbiota composition. After establishing most suitable approach, further examined correlations...
Diet is a strong modifier of microbiome and mucosal microenvironment in the gut. Recently, components western-type diets have been associated with metabolic immune diseases. Here, we studied how high-sugar diet (HSD) consumption influences gut barrier response under steady state conditions mouse model acute colitis. We found that HSD significantly increased permeability, spleen weight, neutrophil levels spleens healthy mice. Subsequent dextran sodium sulfate administration led to severe In...
Psoriatic patients have altered microbiota, both in the intestine and on skin. It is not clear, however, whether this a cause or consequence of disease. In study, using an experimental mouse model psoriasis induced by imiquimod (IMQ), we show that oral treatment with broad spectrum antibiotics (MIX) metronidazole (MET) alone mitigates severity skin inflammation through downregulation Th17 immune response conventional mice. Since some antibiotics, including MET, can influence system...
Recurrent aphthous stomatitis (RAS) is the most common disease of oral mucosa, and it has been recently associated with bacterial fungal dysbiosis. To study this link further, we investigated microbial shifts during RAS manifestation at an ulcer site, in its surroundings, unaffected compared healed mucosa patients healthy controls. We sampled microbes from five distinct sites cavity. The one site pronounced differences alpha beta diversity between controls was lower labial mucosa. Detailed...
Crohn’s disease (CD), ulcerative colitis (UC) and inflammatory bowel (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of (IBD)-gut dysbiosis, gut barrier failure immune system dysregulation. While clinical differences among them are well known, the underlying less explored. To gain an insight into IBD pathogenesis to find a specific biomarker pattern for each them, we used protein array, ELISA flow cytometry analyze serum biomarkers...
Aims Gestation is linked to changes in gut microbiota composition and function. Since gestational diabetes mellitus (GDM) can develop at any time of the pregnancy, we stratified women into four groups according test used for diagnosis. We focused on pattern early pregnancy detect which could be later GDM development. Methods collected stool samples from 104 pregnant including obese individuals (first trimester body mass index median was 26.73). divided routine screening fasting plasma...
The canonical Wnt signaling pathway controls the continuous renewal of intestinal epithelium and specification epithelial cell lineages. Tcf4, a nuclear mediator signaling, is essential for differentiation maintenance Paneth cells in small intestine. Its deficiency associated with reduced expression key α-defensins, highlighting its role host-microbe interactions. However, exact function Tcf4 specifying secretory lineage contribution to antimicrobial peptide production remain incompletely...
IgA nephropathy (IgAN) is the dominant type of primary glomerulonephritis worldwide. However, IgAN rarely affects African Blacks and uncommon in Americans. Polymeric IgA1 with galactose-deficient hinge-region glycans recognized as auto-antigen by glycan-specific antibodies, leading to formation circulating immune complexes nephritogenic consequences. Because human B cells infected vitro Epstein-Barr virus (EBV) secrete IgA1, we examined peripheral blood from adult patients, relevant...
Inflammatory bowel diseases (IBD) are chronic disorders of the gastrointestinal tract that have been linked to microbiome dysbiosis and immune system dysregulation. We investigated longitudinal effect anti-TNF therapy on gut microbiota composition specific response commensals in IBD patients. The study included 52 patients tracked over 38 weeks 37 healthy controls (HC). To characterize diversity microbiota, we used amplicon sequencing V3V4 region 16S rRNA for bacterial community ITS1 fungal...
Crohn's disease (CD) and ulcerative colitis (UC) are two forms of inflammatory bowel (IBD), where the role gut but not skin dysbiosis is well recognized. Inhibitors TNF have been successful in IBD treatment, up to a quarter patients suffer from unpredictable adverse events (SkAE). For this purpose, we analyzed temporal dynamics microbiota serum markers inflammation epithelial barrier integrity during anti-TNF therapy SkAE manifestation patients. We observed that signature differs markedly...
Commensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis the impact various on intestinal tissue provides an important insight into function this organ. We used cDNA microarrays determine gene expression signature mucosa isolated from small intestine and colon germ-free (GF) mice animals monoassociated with two E. coli strains. The results were compared data obtained in conventionally reared (CR) mice. In addition, we analyzed organoids derived...
Oxidative stress, oxidative DNA damage and resulting mutations play a role in colorectal carcinogenesis. Impaired equilibrium between formation, antioxidant status, repair capacity is responsible for the accumulation of genetic genomic instability. The lesion-specific glycosylases, e.g., hOGG1 MUTYH, initiate damage. Hereditary syndromes (MUTYH-associated polyposis, NTHL1-associated tumor syndrome) with germline causing loss-of-function base excision serve as straight forward evidence on its...
Tumor necrosis factor-alpha (TNF-α) agonists revolutionized therapeutic algorithms in inflammatory bowel disease (IBD) management. However, approximately every third IBD patient does not respond to this therapy the long term, which delays efficient control of intestinal inflammation. We analyzed power serum biomarkers predict failure anti-TNF-α. collected 38 patients at prescription and weeks later them with relation response (no-, partial-, full response). used enzyme-linked immunosorbent...
Aims: Epstein-Barr virus (EBV) targets predominantly B cells and these could acquire new phenotype characteristics. Here we analyzed whether EBV-infected -uninfected from healthy subjects differ in proportion of dominant phenotypes, maturation stage, homing receptors expression. Methods: were identified by flow cytometry using fluorophore-labeled EBV RNA-specific DNA probes combined with antibody to surface lineage markers, integrins, chemokine receptors, immunoglobulin isotypes, including...
Background Ustekinumab, is a new therapy for patients with IBD, especially suffering from Crohn’s disease (CD) who did not respond to anti-TNF treatment. To shed light on the longitudinal effect of ustekinumab immune system, we investigated skin and gut microbiota composition, specific response commensals, various serum biomarkers. Methodology/Principal findings We recruited 11 IBD were monitored over 40 weeks 39 healthy controls (HC). found differences in concentrations levels...
Topic: 20. Lymphoma Biology & Translational Research Background: The Non-Hodgkin’s lymphomas (NHL) evolution, progression and the response to therapy could be connected with immune evasion mechanism. Microbiota composition is associated host regulation. It studied in different diseases, context of types immunotherapy reactivity. data on oral microbiota (oralM) as well gastrointestinal (gutM) NHL are still limited (Diefenbach et al., 2021 Yuan 2021). We have decided run a prospective study...
The single-layer epithelium of the gastrointestinal tract is a dynamically renewing tissue that ensures nutrient absorption, secretory and barrier functions involved in immune responses. basis for this homeostatic renewal Wnt signaling pathway. Blocking pathway can lead to epithelial damage, while its abnormal activation result development intestinal tumors. In study, we investigated dynamics cells tumorigenesis using conditional mouse model. Using single-cell bulk RNA sequencing...
The gut microbiota influences the reactivity of immune system, and
Background: Microbiota (MB) participates on the host immune system regulation. There is data that MB could be connected with immunotherapy effect in several cancers e.g. melanoma. It may play important role pathogenesis or therapy of lymphoma as well. however still limited knowledge about diversity different subtypes especially large B-cell immune-privileged sites (LBCL-IPS). We present first prospective microbiota project lymphoma. Methods: have analyzed 10 healthy donors (CON, age med. 67)...
Abstract Background Crohn’s disease (CD) and ulcerative colitis (UC) are two forms of inflammatory bowel (IBD), where gut but not skin dysbiosis is well recognized. Tumor-necrosis factor alpha (TNFα) blockers have been successful in IBD treatment, up to a quarter patients suffer from adverse events (SkAE), for which we do tool predict them. Therefore, examined the microbiota composition at several body sites by 16S bacterial profiling. Moreover, quantified 22 serum markers inflammation...