A5084593874- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Telomeres, Telomerase, and Senescence
- Science, Research, and Medicine
- Acute Lymphoblastic Leukemia research
- Fungal Biology and Applications
- Radiation Therapy and Dosimetry
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Genetically Modified Organisms Research
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Genomic variations and chromosomal abnormalities
- Complementary and Alternative Medicine Studies
- Peripheral Neuropathies and Disorders
- Plant Genetic and Mutation Studies
- Medicinal plant effects and applications
- DNA and Nucleic Acid Chemistry
- Health, Environment, Cognitive Aging
Czech Academy of Sciences
2015-2024
Czech Academy of Sciences, Institute of Experimental Medicine
2015-2024
Charles University
2015-2024
General University Hospital in Prague
2018-2019
Epidemiological prospective studies have shown that increased chromosomal aberrations (CAs) in peripheral blood lymphocytes may predict cancer risk. Here, we report CAs newly diagnosed 101 colorectal, 87 lung and 158 breast patients corresponding healthy controls. Strong differences distributions of aberrant cells (ACs), CAs, chromatid-type (CTAs) chromosome-type (CSAs) were observed as compared to In colorectal (CRC) patients, only CTAs significantly elevated. Binary logistic regression,...
Human cancers are often associated with numerical and structural chromosomal instability. Structural aberrations (CAs) in peripheral blood lymphocytes (PBL) arise as consequences of direct DNA damage or due to replication on a damaged template. In both cases, repair is critical inter-individual differences its capacity probably corresponding genetic variations. We investigated functional variants genes (base nucleotide excision repair, double-strand break repair) relation CAs, chromatid-type...
DNA repair processes are involved in both the onset and treatment efficacy of colorectal cancer (CRC). A change a single nucleotide causing an amino acid substitution corresponding protein may alter efficiency repair, thus modifying CRC susceptibility clinical outcome. We performed candidate gene approach order to analyze association non-synonymous polymorphisms (nsSNPs) genes covering main pathways with risk outcome modifications. Our were selected according foremost genomic functional...
Several studies have suggested a possible relationship between polymorphic variants of the taste receptors genes and acceptance, liking intake food beverages. In last decade investigators attempted to link individual ability 6-n-propylthiouracil (PROP) sensations, such as astringency bitterness, elicited by wine or its components, but with contradictory results. We used genotype instead phenotype (responsiveness PROP other tastants), test relation genetic variability perception...
MicroRNAs (miRNAs) regulate gene expression in a tissue-specific manner. However, little is known about the miRNA changes induced by therapy rectal cancer (RC) patients. We evaluated levels before and after identified specific signatures reflecting disease course treatment responses of RC First, were assessed next-generation sequencing two plasma samplings (at time diagnosis year after) from 20 MiR-122-5p miR-142-5p classified for subsequent validation extracellular vesicles (EVs) on an...
Abstract Development of lymphoid progenitors requires a coordinated regulation gene expression, DNA replication, and rearrangement. Chromatin-remodeling activities directed by SWI/SNF2 superfamily complexes play important roles in these processes. In this study, we used conditional knockout mouse model to investigate the role Smarca5, member ISWI subfamily such complexes, early lymphocyte development. Smarca5 deficiency results developmental block at DN3 stage αβ thymocytes pro-B B cells...
Abstract Disruption of telomere length (TL) homeostasis in peripheral blood lymphocytes has been previously assessed as a potential biomarker breast cancer (BC) risk. The present study addressed the relationship between lymphocyte TL (LTL), prognosis and clinicopathological features BC patients since these associations are insufficiently explored at present. LTL was measured 611 154 healthy controls using monochrome multiplex quantitative Polymerase Chain Reaction assay. In addition, we...
Disruption of genomic integrity due to deficient DNA repair capacity and telomere shortening constitute hallmarks malignant diseases. Incomplete or double-strand breaks (DSB) is manifested by chromosomal aberrations their frequency reflects inter-individual differences response exposure mutagenic compounds. In this study, we investigated in peripheral blood lymphocytes (PBL) from newly diagnosed cancer patients, including 47 breast (BC) 44 colorectal (CRC) patients 90 matched healthy...
Chromosomal aberrations (CAs) in human peripheral blood lymphocytes (PBL) measured with the conventional cytogenetic assay have been used for biomonitoring of genotoxic exposure decades. CA frequency is a marker cancer susceptibility. Previous studies shown associations between genetic variants metabolic pathway, DNA repair and major mitotic checkpoint genes CAs. We conducted genome‐wide association study on 576 individuals from Czech Republic Slovakia followed by replication two different...
DNA damage and unrepaired or insufficiently repaired double-strand breaks as well telomere shortening contribute to the formation of structural chromosomal aberrations (CAs). Non-specific CAs have been used in monitoring individuals exposed potential carcinogenic chemicals radiation. The frequency peripheral blood lymphocytes (PBLs) has associated with cancer risk association also found incident patients. include chromosome-type (CSAs) chromatid-type (CTAs) their sum CAtot. In present study,...
Abstract Non-specific structural chromosomal aberrations (CAs) observed in peripheral blood lymphocytes of healthy individuals can be either chromosome-type (CSAs) or chromatid-type (CTAs) depending on the stage cell division they are induced and mechanism formation. It is important to study genetic basis instability as it a marker genotoxic exposure predictor cancer risk. For that purpose, we conducted two genome-wide association studies (GWASs) presence absence apparent from Czech Republic...