A5072003219- Chromatin Remodeling and Cancer
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Education, Psychology, and Social Research
- Renal and related cancers
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Nutrition, Genetics, and Disease
- interferon and immune responses
Charles University
2019-2024
ISWI chromatin remodeling ATPase SMARCA5 (SNF2H) is a well-known factor for its role in regulation of DNA access via nucleosome sliding and assembly. transcriptionally inhibits the myeloid master regulator PU.1. Upregulation was previously observed CD34+ hematopoietic progenitors acute leukemia (AML) patients. Since high levels are necessary intensive cell proliferation cycle progression developing stem progenitor cells mice, we reasoned that removal enzymatic activity could affect cycling...
Smarca5, an ATPase of the ISWI class chromatin remodelers, is a key regulator structure, cell cycle and DNA repair. Smarca5 deregulated in leukemia breast, lung gastric cancers. However, its role oncogenesis not well understood. Chromatin remodelers often play dosage-dependent roles cancer. We therefore investigated epigenomic phenotypic impact controlled stepwise attenuation function context primary transformation, process relevant to tumor formation. Upon conditional single- or...
Abstract Development of lymphoid progenitors requires a coordinated regulation gene expression, DNA replication, and rearrangement. Chromatin-remodeling activities directed by SWI/SNF2 superfamily complexes play important roles in these processes. In this study, we used conditional knockout mouse model to investigate the role Smarca5, member ISWI subfamily such complexes, early lymphocyte development. Smarca5 deficiency results developmental block at DN3 stage αβ thymocytes pro-B B cells...
Abstract The formation of hematopoietic cells relies on the chromatin remodeling activities ISWI ATPase SMARCA5 (SNF2H) and its complexes. Smarca5 null conditional alleles have been used to study functions in embryonic organ development mice. These mouse model phenotypes vary from lethality constitutive knockout less severe observed tissue-specific deletions, e.g., system. Here we show that, a gene dosage-dependent manner, hypomorphic allele ( S5 tg ) can rescue not only developmental arrest...