A5091200782- Cerebrospinal fluid and hydrocephalus
- Epilepsy research and treatment
- Education, Psychology, and Social Research
- Cardiac, Anesthesia and Surgical Outcomes
- Peripheral Artery Disease Management
- Aortic aneurysm repair treatments
- Glioma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Neurological disorders and treatments
- Vagus Nerve Stimulation Research
- Aortic Disease and Treatment Approaches
- Neurosurgical Procedures and Complications
- Traumatic Brain Injury and Neurovascular Disturbances
- Thyroid Cancer Diagnosis and Treatment
- EEG and Brain-Computer Interfaces
- Spinal Fractures and Fixation Techniques
- Cerebrovascular and Carotid Artery Diseases
- Intracranial Aneurysms: Treatment and Complications
- Fetal and Pediatric Neurological Disorders
- Vascular Procedures and Complications
- Pharmacological Effects and Toxicity Studies
- Head and Neck Surgical Oncology
- Herpesvirus Infections and Treatments
- Venous Thromboembolism Diagnosis and Management
- Spinal Dysraphism and Malformations
University of Alabama at Birmingham
2016-2025
University of Alabama
2025
Institute of Endocrinology
1995-2024
Charles University
1995-2024
Campden BRI (United Kingdom)
2024
Masaryk University
2013-2023
St. Anne's University Hospital Brno
2012-2023
Czech Technical University in Prague
2012-2023
RELX Group (Netherlands)
2023
The Ohio State University
2022
Congenital cytomegalovirus (CMV) infection is an important cause of hearing loss, and most infants at risk for CMV-associated loss are not identified early in life because failure to test the infection. The standard assay newborn CMV screening rapid culture performed on saliva specimens obtained birth, but this cannot be automated. Two alternatives--real-time polymerase-chain-reaction (PCR)-based testing a liquid-saliva or dried-saliva specimen birth--have been developed.In our prospective,...
Cytomegalovirus (CMV) is recognized as the most common congenital viral infection in humans and an important cause of morbidity mortality immunocompromised hosts. This recognition clinical importance invasive CMV disease setting immunodeficiency children with has led to development new diagnostic procedures for rapid identification CMV. Diagnosis acute maternal by presence immunoglobulin (Ig)M low-avidity IgG requires confirmation fetal infection, which typically performed using polymerase...
Cytomegalovirus (CMV) reinfections have been associated with damaging congenital infection and adverse outcomes in transplant recipients. To determine the frequency of risk factors for CMV reinfections, 205 seropositive women were followed up prospectively. The appearance new antibody specificity against 1 4 polymorphic epitopes was considered as evidence reinfection. Approximately one-third study participants (59 [29%] 205) noted to reinfection during follow-up. None exposure Women...
Viral culture of urine or saliva has been the gold standard technique for diagnosis congenital cytomegalovirus (CMV) infection. Results rapid and polymerase chain reaction (PCR) analysis specimens from 80 children were compared to determine clinical utility a real-time PCR assay CMV positive in 98.8% specimens. Three PCR-positive samples negative. concordant 78 (97.5%). Two These findings demonstrate that performs as well diagnosing infection are easier collect. Because also offers more...
This article presents an adaptive quadrature phase-locked loop (AQPLL) for improving the dynamic performance of sensorless high-speed permanent magnet synchronous motors (PMSMs). A conventional (QPLL) operating with a fixed bandwidth tends to have tradeoff between antidisturbance and phase delay. The AQPLL can start operate low bandwidth, while is adapted during motor acceleration in real time. Thus, position estimation error quickly minimized, leading correctly decoupled rotor currents...
Abstract Background Stromal fibroblasts associated with in situ and invasive breast carcinoma differ phenotypically from normal epithelium, these alterations carcinoma-associated (CAF) may promote carcinogenesis cancer progression. A better understanding of the changes that occur during their influence on epithelial cell growth behavior could lead to novel strategies for prevention treatment cancer. To this end, effect CAF breast-associated (NAF) cells representative pre-neoplastic disease...
The hedgehog pathway regulates epithelial-mesenchymal interactions, differentiation, proliferation and survival during development. Stimulation of signaling induces carcinogenesis or promotes cell in cancers multiple organs. Using real-time, quantitative PCR, laser capture microdissection, immunohistochemistry, distinctive patterns expression the members patched 1 (PTCH1), smoothened, GLI1, GLI2 3 ligands were identified for epithelial cells stromal fibroblasts benign breast cancer. Hedgehog...
This study was designed to determine whether elevated viral load in infants and young children is associated with congenital cytomegalovirus (CMV)-related hearing loss.Blood samples were obtained from 135 CMV infection. DNA the peripheral blood quantitated a real-time polymerase chain reaction assay. Viral measurements analyzed 3 different age groups (<2 months, 2-12 12-36 months).In symptomatic asymptomatic infection, levels not between deficit those normal all groups. In positive...
Cytomegalovirus (CMV), the most common cause of congenital infection, exhibits extensive genetic variability. We sought to determine whether multiple CMV strains can be transmitted fetus and describe distribution genotypes in saliva, urine, blood.Study subjects consisted a convenience sampling 28 infants found CMV-positive on newborn screening as part an ongoing study. Genotyping was performed saliva specimens obtained during blood at later time point within first 3 weeks life.Six (21.4%)...
ABSTRACT Infection and reinfection with multiple cytomegalovirus (CMV) strains have been shown to occur in immunocompromised individuals, sexually transmitted disease clinic attendees, children attending day care centers. To characterize the CMV diversity healthy seropositive 16 PCR-positive specimens from 113 women were analyzed for glycoprotein gN gB genotypes by cloning, followed nucleotide sequencing of plasmid DNA and/or restriction fragment length polymorphism (RFLP). The results...
Stromal fibroblasts influence the behavior of breast epithelial cells. Fibroblasts derived from normal (NAF) inhibit growth, whereas carcinomas (CAF) have less growth inhibitory capacity and can promote growth. We sought to identify molecules that are differentially expressed in NAF versus CAF potentially responsible for their different regulatory abilities. To determine contribution soluble fibroblast-epithelial interactions, were grown 3D, transwell or direct contact co-cultures with...
Background. Circulating immune complexes (CIC) containing galactose (Gal)-deficient IgA1 from adults with IgA nephropathy (IgAN) induce proliferation of cultured mesangial cells, but activities CIC pediatric patients the disease have not been studied. Methods. different sizes were isolated sera and adult IgAN their effects on human cells (MC) assessed by measuring cellular proliferation, expression IL-6 IL-8 laminin phosphotyrosine signaling. Results. Large (>800 kDa) Gal-deficient...
Growing evidence shows that high maternal HIV seroprevalence could drive an excess burden of congenital cytomegalovirus (CMV). We show for the first time a prevalence CMV among HIV-exposed newborns in sub-Saharan Africa, despite universal prenatal antiretroviral prophylaxis.
As part of the CMV and Hearing Multicenter Screening (CHIMES) study, 72,239 newborns were screened for cytomegalovirus by rapid culture real-time PCR saliva samples. Of 266 infants with congenital infection, discordance between was observed in 14 children, 13 identified only PCR, demonstrating superiority assay.
Summary: Purpose: To investigate the effects of acute vagal nerve stimulation (VNS) on interictal epileptiform discharges (IEDs). Methods: Fifteen epilepsy patients, all whom had been treated with VNS for ≥6 months, entered study. In each subject, absolute number IEDs was counted at baseline period (BP), (SP), six interstimulation periods (IPs), and prestimulation (PP), by using an original paradigm. The BP PP compared SP IPs. results were correlated other variables (the duration VNS, value...
Viruria and DNAemia patterns were investigated in 205 seroimmune women enrolled a prospective cytomegalovirus (CMV) reinfection study. CMV DNA was detected at least once urine blood specimens from 83% 52% of patients, respectively. At baseline, 39% patients had viruria, 24% DNAemia. Intermittent viruria viremia observed throughout the There no differences baseline positivity by polymerase chain reaction or longitudinal between with without serological evidence reinfection. In young...