A5014894298- Cytomegalovirus and herpesvirus research
- Acute Myeloid Leukemia Research
- T-cell and Retrovirus Studies
- Retinoids in leukemia and cellular processes
- Neonatal Health and Biochemistry
- Animal Disease Management and Epidemiology
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Vector-Borne Animal Diseases
- Mesenchymal stem cell research
- Parvovirus B19 Infection Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Herpesvirus Infections and Treatments
- Blood groups and transfusion
- Cancer Cells and Metastasis
- Healthcare during COVID-19 Pandemic
- Immunodeficiency and Autoimmune Disorders
- Ocular Diseases and Behçet’s Syndrome
- Maternal and Neonatal Healthcare
- Immune cells in cancer
- Infant Nutrition and Health
- Systemic Lupus Erythematosus Research
- Bacillus and Francisella bacterial research
- Muscle Physiology and Disorders
- Erythrocyte Function and Pathophysiology
Universidade de São Paulo
2007-2022
Universidade de Ribeirão Preto
2012-2019
Clinics Hospital of Ribeirão Preto
2017
Fundação de Amparo à Pesquisa do Estado de São Paulo
2015-2016
Universidade Brasil
2015
Survivors from sepsis are in an immunosuppressed state that is associated with higher long-term mortality and risk of opportunistic infections. Whether these factors contribute to neoplastic proliferation, however, remains unclear. Tumor-associated macrophages (TAM) can support malignant cell survival, angiogenesis. We addressed the relationship between post-sepsis state, tumor progression TAM accumulation, phenotypic genetic profile, using a mouse model resolution then B16 melanoma mice. In...
Mesenchymal stromal cells (MSCs) are cultured that can give rise to mature mesenchymal under appropriate conditions and secrete a number of biologically relevant molecules may play an important role in regenerative medicine. Evidence indicates pericytes (PCs) correspond stem vivo MSCs when cultured, but comparison between the gene expression profiles PCs (cPCs) is lacking. We have devised novel methodology isolate from human adipose tissue compared cPCs obtained through traditional methods....
Abstract A case‐control study design was used in order to compare the distribution of human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes 48 mothers 49 congenitally infected infants with that observed 144 146 uninfected genetic variation HCMV strains and maternal‐fetal transmission. Congenital infection characterized by DNA detection virus isolation from two urine or saliva samples collected prior third week life. Genotyping carried out a polymerase chain reaction‐restriction fragment...
Interleukin‐18 (IL‐18) and interferon‐gamma (IFN‐γ) exert important functions in both innate adaptive immune responses against intracellular pathogens viruses. Previous studies suggested that host genetic factors, including cytokines gene polymorphisms, could be involved the pathogenesis of human T‐cell leukemia virus type 1 (HTLV‐1)‐associated myelopathy/tropical spastic paraparesis (HAM/TSP). Thus, we analyzed −137C/G −607A/C IL‐18 promoter +874T/A IFN‐γ DNA samples from 98 HTLV‐1‐infected...
In approximately 15% of patients with acute myeloid leukemia (AML), total and phosphorylated EGFR proteins have been reported to be increased compared healthy CD34+ samples. However, it is unclear if this subset would benefit from signaling pharmacological inhibition. Pre-clinical studies on AML cells provided evidence the pro-differentiation benefits inhibitors when combined ATRA or ATO in vitro. Despite success treatment promyelocytic (APL), therapy-associated resistance observed 5-10%...
Most human T-lymphotropic virus type 1 (HTLV-1)-infected patients remain asymptomatic throughout life. The factors associated with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) development have not been fully elucidated; immunological and genetic may be involved. association of 14 bp INS/DEL HLA-G polymorphism HTLV-1 infection susceptibility has reported previously. Here, other polymorphic sites at the 3′-UTR (14-bp D/I, +3003C/T, +3010C/G, +3027A/C, +3035C/T, +3142C/G,...
The role of the human cytomegalovirus (HCMV) infection in individuals with hemoglobinopathies is unclear. Our objective was to examine molecular and genotypic characteristics HCMV patients sickle cell disease, beta-thalassemia major, volunteer blood donors by viral load quantitation, glycoprotein B (gB) genotyping, phylogenetic analysis. disease demonstrated highest DNA prevalence (13.8%), followed major (7.6%), (3%). characterized a low mean (3.8×103 copies/mL), but infections higher copy...
The emergence of ganciclovir (GCV) resistance during the treatment human cytomegalovirus (HCMV) infection is a serious clinical challenge, and associated with high morbidity mortality. In this case report, we describe two consecutive mutations (A594V L595W) related to GCV in patient HCMV retinitis long-term HIV progression after approximately 240 days use. Following diagnosis retinitis, introduction did not result viral load reduction. detected appeared late treatment, propose that other...
Accurate diagnostic tests are powerful tool to control the spread of human T-lymphotropic virus (HTLV) infection. In Brazil, currently applied algorithm for HTLV-1/2 screening is based on serological (enzyme-linked immunosorbent assay followed by Western Blot). However this unsuitable due its high cost and elevated rate Blot (WB) indeterminate results. Nevertheless, molecular techniques such as real-time PCR (qPCR) can overlap these drawbacks because their sensitivity specificity. Several...
HAM/TSP is an inflammatory manifestation of central nervous system caused by HTLV-1 and the mechanism development no well elucidated. Currently, a promising approach on physiopathogenesis viral infections has been evaluation microRNAs (miRNAs) role. There are few data involving CD4+ T cells miRNA expression in infection as establishment. To identify miRNAs differentially expressed among non-infected individuals (CT), asymptomatic (HAC) patients we applied quantitative real time PCR. The...
e22107 Background: Sepsis surviving mice (SSM) present increased tumor progression. We assessed the role of tumor-associated macrophage (TAM) and CXCL12/CXCR4 in that scenario. Methods: C57/BL6 were submitted to cecal ligation puncture (CLP) treated with ertapenem (20 mg/kg, ip. - 6 h after CLP then each 12 for 3 days). SSM or naïve inoculated B16-F10 (30,000 cells, sc., 15 days CLP). Tumor progression, survival pulmonary metastasis measured. Fourteen inoculation (D+14), killed TAM...
Abstract The nuclear SET domain (NSD) protein lysine methyltransferases (KMT) family is composed of three members, NSD1/KMT3B, NSD2/WHSC1/MMSET, and NSD3/WHSC1L1, which regulate gene expression through methylation 36 histone H3 (H3K36). NSD2 overexpression was reported in multiple myeloma with t(4;14)/IgH-MMSET. NSDs profile unknown acute leukemias; however, NSD1 NSD3 were described to be fused the nucleoporin 98 (NUP98) rare AML myelodysplastic syndrome cases both fusion proteins associated...
Background: Recent data pointed to the association of disease progression and relapse in acute myeloid leukemia (AML) with evidence functional mitochondria (mt), reactive oxygen species (ROS) transference from bone marrow stromal cells (BMSCs) blasts cells. Nevertheless, differential performances this process among normal leukemic‐BMSCs co‐culture systems were not well established. Aims: To evaluate presence a favoring microenvironment for AML's survival provided by through reduction...