In this paper, we discuss an impact of thin titanium dioxide (TiO(2)) coatings on refractive index (RI) sensitivity and biofunctionalization long-period gratings (LPGs). The TiO(2) overlays the LPG surfaces have been obtained using atomic layer deposition (ALD) method. This method allows for a conformal, thickness-controlled, with well-defined optical properties, high-RI films which are highly desired fiber sensors. It has found that LPGs working at dispersion turning point higher order...

Abstract Non-nutritive sweeteners (NNS) are widely employed in foodstuffs. However, it has become increasingly evident that their consumption is associated with bacterial dysbiosis, which, turn, linked to several health conditions, including a higher risk of type 2 diabetes and cancer. Among the NNS, stevia, whose main component rebaudioside A (rebA), gaining popularity organic food market segment. While effect NNS on bacteria been established, impact these viruses (phages) neglected, even...

ABSTRACT The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results stimulation mouse bone marrow-derived dendritic cells (BM-DC) human embryonal kidney (HEK293) stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. produces PS that differ greatly in their structure. polymer L900/2, a high average molecular mass 830 kDa, is branched heteropolysaccharide unique repeating unit consisting seven sugar residues...

The Lactobacillus casei strain, LOCK 0919, is intended for the dietary management of food allergies and atopic dermatitis (LATOPIC® BIOMED). use a probiotic to modulate immune responses an interesting strategy solving imbalance problems gut microflora that may lead various disorders. However, exact bacterial signaling mechanisms underlying such modulations are still far from being understood. Here, we investigated variations in chemical compositions immunomodulatory properties...

Tail tubular protein A (TTPA) is a structural tail of Klebsiella pneumoniae bacteriophage KP32, and responsible for adhering the to host cells. For first time, we found that TTPA also exhibits lytic activity towards capsular exopolysaccharide (EPS) multiresistant clinical strain pneumoniae, PCM2713, thus should be regarded as dual-function macromolecule both enzymatic actions. Here, present our crystallographic studies TTPA. was crystallized X-ray diffraction data were collected resolution...

Characteristic changes in the microbiota biostructure and a decreased tolerance to intestinal bacteria have been associated with inflammatory bowel disease (IBD). However, few studies examined constituents of microbiota, including surface molecules bacteria, healthy IBD subsets. Here, we compare chemical structures immunomodulatory properties exopolysaccharides (EPS) lactobacilli isolated from mice induced (IBD "+") versus those "-"). Classical structural analyses were performed using...

This paper presents a novel application of highly sensitive sensor based on long-period gratings (LPGs) coated with T4 bacteriophage adhesin for Gram-negative bacteria detection.We show here, that the evidently recognizes Escherichia coli K-12 (PCM2560), whereas in reference tests -ELISA and BIAcore -the results are questionable.For LPGs resonant wavelength shift observed E. was approximately half measured E.coli B (positive control).The readings (RU) were at 10% level signal obtained E...

In this paper, we study a label-free detection specificity of an optical fiber long-period grating (LPG) biosensor working near the dispersion turning point higher order cladding modes. The LPG sensor is functionalized with bacteriophage adhesin and tested specific nonspecific bacteria dry weights. It shown that such able to selectively bind bacterial strains. experiments weights E. coli B were used as positive test K12 Salmonella enterica negative tests. resonance wavelength shift induced...

Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and pseudotuberculosis. Pigs other wild domestic animals are reservoirs for these bacteria. Infection usually spread to humans ingestion contaminated food. considered a rare disease, but recent studies indicate that it overlooked in the diagnostic process therefore infections with this bacterium not often identified. Reliable diagnosis culturing...

Abstract Pseudomonas aeruginosa is an opportunistic pathogen with a capacity to develop antibiotic resistance, which underlies larger proportion of hospital-acquired infections and higher morbidity mortality, compared other bacterial infections. Effective novel approaches for treatment induced by this are therefore necessary. Phage therapy represents promising alternative solution eradicate antibiotic-resistant pathogens. Here, we investigated phage protein efficacy against multi-drug...

Summary Here, we compared the abilities of polysaccharides L900/2 and L900/3, which were previously isolated from Lactobacillus rhamnosus LOCK 0900, to modulate immune response bystander antigens in a mouse model ovalbumin ( OVA ) sensitization. In vivo , both reduced levels ‐specific IgE, IgE‐dependent basophil degranulation IgG2a antibodies, but had no effect on IgA or IgG1. Interestingly, triggered recall cellular responses with distinct properties. L900/3 significantly suppressed...

In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tubular A-TTPAgp31 TTPAgp44-have been considered as structural Klebsiella pneumoniae bacteriophages KP32 KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose well Red-starch. The activity 1 µM protein was calculated 47.6 milli-Units/assay relating α-amylase activity. It degrades capsular polysaccharides (cPS),...

The elongation factor Tu has been identified as one of the most immunoreactive proteins that was recognized by human sera GBS positive patients. In this paper, we present polypeptide-specific epitopes bacterial protein are antibodies: 28LTAAITTVLARRLP41 (peptide no. 3) and 294GQVLAKPGSINPHTKF309 21). To determine shortest amino acid sequence antibodies, truncation peptide libraries were prepared using PEPSCAN method. analysis immunoreactivity peptides with negative women revealed 306HTKF309....

The genus Lactobacillus belongs to a large heterogeneous group of low G + C Gram-positive anaerobic bacteria, which are frequently used as probiotics. health-beneficial effects, in particular the immunomodulation effect, probiotics depend on strain and dose used. Strain variations may be related diversity cell surface architecture bacteria ability express specific antigens or secrete compounds. use probiotic requires comprehensive understanding its effect host immune system. To evaluate...

The interaction between the T4 bacteriophage gp37 adhesin and bacterial lipopolysaccharide (LPS) is a well-studied system, however, affinity strength of haven't been analyzed so far. Here, we use atomic force microscopy to determine its receptor, namely LPS taken from wild strain E. coli B. As negative controls used LPSs O111:B Hafnia alvei. To study an AFM tip modified with was scan surfaces mica covered one three different LPSs. Using correlation surface topography images tip-surface could...