Ewa Brzozowska

ORCID: 0000-0003-0922-3625
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About
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Research Areas
  • Bacteriophages and microbial interactions
  • Probiotics and Fermented Foods
  • Mechanical and Optical Resonators
  • Genomics and Phylogenetic Studies
  • Photonic and Optical Devices
  • Advanced Fiber Optic Sensors
  • Monoclonal and Polyclonal Antibodies Research
  • Gut microbiota and health
  • Neonatal and Maternal Infections
  • Advanced Biosensing Techniques and Applications
  • Streptococcal Infections and Treatments
  • Yersinia bacterium, plague, ectoparasites research
  • Biosensors and Analytical Detection
  • Glycosylation and Glycoproteins Research
  • Antimicrobial Peptides and Activities
  • Bacterial Genetics and Biotechnology
  • Microbial infections and disease research
  • Infant Nutrition and Health
  • RNA and protein synthesis mechanisms
  • Microbial Metabolites in Food Biotechnology
  • Polysaccharides and Plant Cell Walls
  • Pneumonia and Respiratory Infections
  • Digestive system and related health
  • Advanced biosensing and bioanalysis techniques
  • Acoustic Wave Resonator Technologies

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2015-2025

Polish Academy of Sciences
2014-2025

Medical University of Białystok
2022

Warsaw University of Technology
2014

In this paper, we discuss an impact of thin titanium dioxide (TiO(2)) coatings on refractive index (RI) sensitivity and biofunctionalization long-period gratings (LPGs). The TiO(2) overlays the LPG surfaces have been obtained using atomic layer deposition (ALD) method. This method allows for a conformal, thickness-controlled, with well-defined optical properties, high-RI films which are highly desired fiber sensors. It has found that LPGs working at dispersion turning point higher order...

10.1364/oe.23.008441 article EN cc-by Optics Express 2015-03-24

Abstract Non-nutritive sweeteners (NNS) are widely employed in foodstuffs. However, it has become increasingly evident that their consumption is associated with bacterial dysbiosis, which, turn, linked to several health conditions, including a higher risk of type 2 diabetes and cancer. Among the NNS, stevia, whose main component rebaudioside A (rebA), gaining popularity organic food market segment. While effect NNS on bacteria been established, impact these viruses (phages) neglected, even...

10.1038/s41598-025-85186-w article EN cc-by Scientific Reports 2025-01-08

ABSTRACT The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results stimulation mouse bone marrow-derived dendritic cells (BM-DC) human embryonal kidney (HEK293) stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. produces PS that differ greatly in their structure. polymer L900/2, a high average molecular mass 830 kDa, is branched heteropolysaccharide unique repeating unit consisting seven sugar residues...

10.1128/aem.02104-14 article EN Applied and Environmental Microbiology 2014-08-09

The Lactobacillus casei strain, LOCK 0919, is intended for the dietary management of food allergies and atopic dermatitis (LATOPIC® BIOMED). use a probiotic to modulate immune responses an interesting strategy solving imbalance problems gut microflora that may lead various disorders. However, exact bacterial signaling mechanisms underlying such modulations are still far from being understood. Here, we investigated variations in chemical compositions immunomodulatory properties...

10.1093/glycob/cww047 article EN cc-by-nc Glycobiology 2016-04-21

Tail tubular protein A (TTPA) is a structural tail of Klebsiella pneumoniae bacteriophage KP32, and responsible for adhering the to host cells. For first time, we found that TTPA also exhibits lytic activity towards capsular exopolysaccharide (EPS) multiresistant clinical strain pneumoniae, PCM2713, thus should be regarded as dual-function macromolecule both enzymatic actions. Here, present our crystallographic studies TTPA. was crystallized X-ray diffraction data were collected resolution...

10.1038/s41598-017-02451-3 article EN cc-by Scientific Reports 2017-05-16

Characteristic changes in the microbiota biostructure and a decreased tolerance to intestinal bacteria have been associated with inflammatory bowel disease (IBD). However, few studies examined constituents of microbiota, including surface molecules bacteria, healthy IBD subsets. Here, we compare chemical structures immunomodulatory properties exopolysaccharides (EPS) lactobacilli isolated from mice induced (IBD "+") versus those "-"). Classical structural analyses were performed using...

10.1038/srep37613 article EN cc-by Scientific Reports 2016-11-21

This paper presents a novel application of highly sensitive sensor based on long-period gratings (LPGs) coated with T4 bacteriophage adhesin for Gram-negative bacteria detection.We show here, that the evidently recognizes Escherichia coli K-12 (PCM2560), whereas in reference tests -ELISA and BIAcore -the results are questionable.For LPGs resonant wavelength shift observed E. was approximately half measured E.coli B (positive control).The readings (RU) were at 10% level signal obtained E...

10.1364/boe.7.000829 article EN cc-by Biomedical Optics Express 2016-02-09

In this paper, we study a label-free detection specificity of an optical fiber long-period grating (LPG) biosensor working near the dispersion turning point higher order cladding modes. The LPG sensor is functionalized with bacteriophage adhesin and tested specific nonspecific bacteria dry weights. It shown that such able to selectively bind bacterial strains. experiments weights E. coli B were used as positive test K12 Salmonella enterica negative tests. resonance wavelength shift induced...

10.1109/jlt.2016.2532466 article EN Journal of Lightwave Technology 2016-02-19

Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and pseudotuberculosis. Pigs other wild domestic animals are reservoirs for these bacteria. Infection usually spread to humans ingestion contaminated food. considered a rare disease, but recent studies indicate that it overlooked in the diagnostic process therefore infections with this bacterium not often identified. Reliable diagnosis culturing...

10.1186/s13568-021-01341-2 article EN cc-by AMB Express 2022-01-06

Abstract Pseudomonas aeruginosa is an opportunistic pathogen with a capacity to develop antibiotic resistance, which underlies larger proportion of hospital-acquired infections and higher morbidity mortality, compared other bacterial infections. Effective novel approaches for treatment induced by this are therefore necessary. Phage therapy represents promising alternative solution eradicate antibiotic-resistant pathogens. Here, we investigated phage protein efficacy against multi-drug...

10.1038/s41598-019-50030-5 article EN cc-by Scientific Reports 2019-09-17

Summary Here, we compared the abilities of polysaccharides L900/2 and L900/3, which were previously isolated from Lactobacillus rhamnosus LOCK 0900, to modulate immune response bystander antigens in a mouse model ovalbumin ( OVA ) sensitization. In vivo , both reduced levels ‐specific IgE, IgE‐dependent basophil degranulation IgG2a antibodies, but had no effect on IgA or IgG1. Interestingly, triggered recall cellular responses with distinct properties. L900/3 significantly suppressed...

10.1111/1751-7915.12606 article EN cc-by Microbial Biotechnology 2017-02-06

In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tubular A-TTPAgp31 TTPAgp44-have been considered as structural Klebsiella pneumoniae bacteriophages KP32 KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose well Red-starch. The activity 1 µM protein was calculated 47.6 milli-Units/assay relating α-amylase activity. It degrades capsular polysaccharides (cPS),...

10.1038/s41598-017-18096-1 article EN cc-by Scientific Reports 2017-12-18

The elongation factor Tu has been identified as one of the most immunoreactive proteins that was recognized by human sera GBS positive patients. In this paper, we present polypeptide-specific epitopes bacterial protein are antibodies: 28LTAAITTVLARRLP41 (peptide no. 3) and 294GQVLAKPGSINPHTKF309 21). To determine shortest amino acid sequence antibodies, truncation peptide libraries were prepared using PEPSCAN method. analysis immunoreactivity peptides with negative women revealed 306HTKF309....

10.3389/fmicb.2018.00125 article EN cc-by Frontiers in Microbiology 2018-02-06

The genus Lactobacillus belongs to a large heterogeneous group of low G + C Gram-positive anaerobic bacteria, which are frequently used as probiotics. health-beneficial effects, in particular the immunomodulation effect, probiotics depend on strain and dose used. Strain variations may be related diversity cell surface architecture bacteria ability express specific antigens or secrete compounds. use probiotic requires comprehensive understanding its effect host immune system. To evaluate...

10.1186/s12866-016-0631-9 article EN cc-by BMC Microbiology 2016-02-09

The interaction between the T4 bacteriophage gp37 adhesin and bacterial lipopolysaccharide (LPS) is a well-studied system, however, affinity strength of haven't been analyzed so far. Here, we use atomic force microscopy to determine its receptor, namely LPS taken from wild strain E. coli B. As negative controls used LPSs O111:B Hafnia alvei. To study an AFM tip modified with was scan surfaces mica covered one three different LPSs. Using correlation surface topography images tip-surface could...

10.1038/s41598-018-29383-w article EN cc-by Scientific Reports 2018-07-13
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