Point-of-care testing (POCT) devices play a crucial role as tools for disease diagnostics, and the integration of biorecognition elements with electronic components into these widens their functionalities facilitates development complex quantitative assays. Unfortunately, biosensors that exploit large conventional IgG antibodies to capture relevant biomarkers are often limited in terms sensitivity, selectivity, storage stability, considerably restricting use POCT real-world applications....

The constant search for new materials applicable in electrochemical sensors and electronic devices is encouraging scientists to turn interdisciplinary research. Particularly interesting this context the combination of biology with chemistry material sciences. Recently, bacteriophages (viruses that infect bacteria) have been increasingly used applications, e.g. as recognition elements biosensors or templates create electrode batteries biofuel cells. Here we present a short review recent...

We investigate the interactions between C-reactive protein (CRP) and new CRP-binding peptide materials using experimental (biological physicochemical) methods with support of theoretical simulations (computational modeling analysis). Three specific peptides (P2, P3, P9) derived from an M13 bacteriophage have been identified phage-display technology. The binding efficiency exposed on phages toward CRP was demonstrated via biological methods. Fibers selected phages/peptides interact...

Point-of-care testing (POCT) devices are currently available for the detection of various important diseases owing to their easy-to-use, portable, and user-friendly nature. However, multistep regent manipulation still impedes performance device end-users. To address this issue, we present a novel sequential flow-through microfluidic that incorporates dual flow behaviors (fast-flow/delayed) into single assessing risk cardiovascular disease (CVDs) using C-reactive protein (CRP) as model...

M13 bacteriophages (in short, phages) have an intrinsic ability to infect Escherichia coli bacteria but can be engineered exhibit specific peptides on their surface which enables them selectively bind diverse targets. Therefore, such phages used as a sensing element in biosensor. Consequently, our aim is assess the effectiveness of sponge-like, laterally spaced, hydrogenated titania nanotubes (S-TiO2-NTs) immobilisation scaffold for electrochemical systems. The electrodes this study were...

With the advent of nanotechnology, carbon nanomaterials such as nanofibers (CNF) have aroused substantial interest in various research fields, including energy storage and sensing. Further improvement their properties might be achieved via application viral particles bacteriophages. In this report, we present a filamentous M13 bacteriophage with point mutation gene VII (pVII-mutant-M13) that selectively binds to form 3D structures. The phage-display technique was utilized for selection...

C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due their inexpensive production, simple engineering, the possibility obtain higher binder density on capture surfaces. Starting from same pre-immune library, we compared selection output resulting two independent panning strategies, one exclusively exploiting phage display...

Bacteriophage material (M13, wild-type) deposited as a film onto poly(ethylene terephthalate) (PET) substrate (6 μm thick with 20 diameter laser-drilled microhole) has been investigated for ion conductivity and ionic current rectification effects potential applications in membranes. The M13 aggregate membrane forms under acidic conditions (in aqueous 10 mM acids) behaves like microporous anion conductor micropores defined by the packing of cylindrical virus particles. Asymmetric deposition...

Point-of-care testing (POCT) devices play a crucial role as tools for disease diagnostics. The integration of biorecognition elements with electronic components into these widens their functionalities and facilitates the development complex quantitative assays. Unfortunately, biosensors that exploit large conventional IgG antibodies to capture relevant biomarkers are often limited in terms sensitivity, selectivity, storage stability, considerably restricting use POCT real-world applications....

This work describes an experimental study towards label-free sensing of C-reactive protein (CRP) – a recognized as inflammation marker. A multimode optical fiber with section its core coated indium tin oxide (ITO) thin film was used sensor. ITO allows for guiding lossy modes and can simultaneously be transparent electrode electrochemical measurements. Therefore, detection based on single sensor possible. Such dual-domain approach is practical, especially when the results in one domains are...

This work discuses indium tin oxide (ITO) coated optical fiber lossy-mode resonance (LMR) sensor working in an electrochemical setup for monitoring of protein binding to the sensor's surface. The mechanism has been observed simultaneously and domain. combined measurement was enabled by electrically conductive ITO overlay. In experiment, biotin molecules have used test collective illustrate effect. It shown that effect is investigated domains at applied potential, qualitative comparison shows...

Indium-tin-oxide-coated long-period fiber grating was used simultaneously as a working electrode and an optical probe sensitive to refractive index changes in combined opto-electrochemical setup. The response of the sensor strongly dependent on applied potential.

Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar those antibodies antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific exposed on pIII present in M13 bacteriophage virion with up 5 copies. Therefore, one particle can bind from five ligands. Here, we discuss dependences...

Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar those antibodies antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific exposed on pIII present in M13 bacteriophage virion with up five copies. Therefore, one particle can bind from ligands. Here, we discuss dependences...

first_page settings Order Article Reprints Font Type: Arial Georgia Verdana Size: Aa Line Spacing:  Column Width:  Background: Open AccessAbstract Bacteriophages-Carbon Nanofibre Modified Electrodes for Biosensing Applications † by Katarzyna Szot-Karpińska 1,*, Damian Kwaśniewski 1, Adam Leśniewski Frank Marken 2 and Joanna Niedziółka-Jönsson 1 Institute of Physical Chemistry, Polish Academy Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland Department University Bath, Bath BA2 7AY, UK *...