A5068596046- Gut microbiota and health
- Celiac Disease Research and Management
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Probiotics and Fermented Foods
- Infant Nutrition and Health
- Inflammatory Bowel Disease
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- T-cell and B-cell Immunology
- Pediatric health and respiratory diseases
- Dermatology and Skin Diseases
- Diet and metabolism studies
- Diabetes and associated disorders
- Escherichia coli research studies
- Eating Disorders and Behaviors
- Psoriasis: Treatment and Pathogenesis
- Microbial Metabolites in Food Biotechnology
- Animal health and immunology
- IL-33, ST2, and ILC Pathways
- Mycobacterium research and diagnosis
Czech Academy of Sciences, Institute of Microbiology
2015-2025
Czech Academy of Sciences
2014-2024
Engineering Academy of the Czech Republic
2024
Charles University
1996-2021
University Hospital Leipzig
2007
Semmelweis University
2007
University College London
2007
Inserm
2007
University of Tripoli
2001
Nihon University
2001
The most important trigger for immune system development is the exposure to microbial components immediately after birth. Moreover, targeted manipulation of microbiota can be used change host susceptibility immune-mediated diseases. Our aim was analyze how differences in early gut colonization patterns composition resident and future reactivity. Germ-free (GF) mice were either inoculated by single oral gavage caecal content or let colonized co-housing with specific pathogen-free (SPF) at...
Background Probiotic bacteria can be used for the prevention and treatment of human inflammatory diseases including bowel (IBD). However, nature active components exact mechanisms this beneficial effects have not been fully elucidated. Our aim was to investigate if lysate probiotic bacterium L. casei DN-114 001 (Lc) could decrease severity intestinal inflammation in a murine model IBD. Methodology/Principal Findings The preventive effect oral administration Lc significantly reduces acute...
The aim was to analyze the influence of intestinal microbiota on development inflammation. We used model chronic inflammation that develops spontaneously in colon conventional severe combined immunodeficiency (SCID) mice restored with CD45 RB(high) subset CD4+T cells isolated from spleen normal BALB/c mice.A CD4+CD45RB(high) subpopulation T purified by magnetic separation (MACS) and transferred into immunodeficient SCID mice. Germ-free (GF) or monoassociated Enterococcus faecalis, SFB...
Dysregulation of innate and adaptive intestinal immune responses to bacterial microbiota is supposed be involved in pathogenetic mechanisms inflammatory bowel diseases (IBDs). We investigated expression Toll-like receptor 2 (TLR2), TLR4, their transmembrane coreceptor CD14 inbiopsy samples from patients with IBD non-inflamed gut mucosa controls. Small intestine colon were obtained by colonoscopy Crohn's disease (CD), ulcerative colitis (UC), Immunohistochemical analysis cryostat sections...
Psoriasis is a chronic inflammatory skin disease in which Th17 cells play crucial role. Since indigenous gut microbiota influences the development and reactivity of immune cells, we analyzed link among microbiota, T formation psoriatic lesions imiquimod-induced murine model psoriasis. To explore role induced inflammation germ-free (GF), broad-spectrum antibiotic (ATB)-treated or conventional (CV) BALB/c C57BL/6 mice. We found that both mice reared GF conditions for several generations CV...
Diet is a major factor determining gut microbiota composition and perturbances in this complex ecosystem are associated with the inflammatory bowel disease (IBD). Here, we used gnotobiotic approach to analyze, how interaction between diet rich proteins influences sensitivity intestinal inflammation murine model of ulcerative colitis. We found that animal protein (aHPD) exacerbates acute dextran sulfate sodium (DSS)-induced colitis while plant (pHPD) does not. The deleterious effect aHPD was...
Brain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it presumably linked to microbiome dysbiosis. We aimed elucidate the gut contribution AN disease pathophysiology. analyzed composition diversity of patients (bacteriome mycobiome) from stool samples before after renourishment, compared them healthy controls. Further, levels assorted neurotransmitters...
Psoriasis is a chronic inflammatory skin disease, whose pathogenesis involves dysregulated interplay among immune cells, keratinocytes and environmental triggers, including microbiota. Bacterial fungal dysbiosis has been recently associated with several immune-mediated diseases psoriasis. In this comprehensive study, we investigated how different sampling sites methods reflect the uncovered microbiota composition. After establishing most suitable approach, further examined correlations...
<i>Background:</i> The development of allergies is a complex in which both composition and influence the intestinal flora play an important role. We observed earlier studies that presence orally administered probiotic <i>Escherichia coli</i> strain intestine stimulated serum local antibody response, decreased pathogens, number infections need for antibiotics. <i>Methods:</i> preventive effect oral colonization after birth with <i>E. was assessed by...
Microbial sensing by Toll-like receptors (TLR) and its negative regulation have an important role in the pathogenesis of inflammation-related cancer. In this study, we investigated signaling gut microbiota development colitis-associated cancer mouse model.Colitis-associated was induced azoxymethane dextran sodium sulfate wild-type interleukin-1 receptor-associated kinase M (IRAK-M)-deficient mice with or without antibiotic (ATB) treatment. Local cytokine production analyzed multiplex assay...
The growing interest in the composition and effects of microbiota raised question how drug pharmacokinetics could be influenced by concomitant application probiotics. aim this study was to find whether probiotic E. coli strain Nissle 1917 (EcN) influences concomitantly taken antiarrhythmic amiodarone (AMI). Live bacterial suspension EcN (or non-probiotic ATCC 25922) applied orally male Wistar rats for seven days, while a control group treated with saline solution. On eighth day,...
The change in the gut microbiome and microbial metabolites a patient suffering from severe enduring anorexia nervosa (AN) diagnosed with small intestinal bacterial overgrowth syndrome (SIBO) was investigated. Microbial dysbiosis is associated both AN SIBO, therefore changes by serial fecal microbiota transplantation (FMT) possible therapeutic modality. This study assessed effects of FMT on barrier function, composition, levels metabolic products. treatment led to improvement which altered...
The microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies inflammatory response in murine model experimental autoimmune uveoretinitis (EAU). We induced EAU germ-free (GF) conventionally housed (CV) mice and CV treated with combination broad-spectrum antibiotics either from day induction one week prior to disease. severity inflammation was assessed by fundus biomicroscopy histology, including immunohistology. immunophenotyping T cells...
Diet is a strong modifier of microbiome and mucosal microenvironment in the gut. Recently, components western-type diets have been associated with metabolic immune diseases. Here, we studied how high-sugar diet (HSD) consumption influences gut barrier response under steady state conditions mouse model acute colitis. We found that HSD significantly increased permeability, spleen weight, neutrophil levels spleens healthy mice. Subsequent dextran sodium sulfate administration led to severe In...
ABSTRACT Human endothelial as well epithelial cells were shown to respond lipopolysaccharides (LPSs). However, the expression and release of CD14 by these so-called CD14-negative have not been studied in detail. We investigated three human intestinal cell lines (ECLs), SW-480, HT-29, Caco-2, for their CD11c/CD18 responsiveness endotoxins. Fluorescence-activated sorter analysis revealed no CD11c/CD18, but there was low membrane-bound on SW-480 ECLs. Both Western blotting reverse...
The aim of this study was to analyze the influence oral administration E. coli Nissle 1917 on systemic humoral and cellular immunity in premature infants. Thirty‐four infants were colonized with a randomized, placebo‐controlled blinded clinical trial. Stool samples analyzed repeatedly for presence administered strain. proliferative response bacterial antigens origin measured whole blood 34 27 noncolonized controls. colonization induced significant increase proliferation cells cultivated...
Abstract Celiac disease is a chronic inflammatory developing in genetically predisposed individuals. Ingested gliadin, the triggering agent of disease, can cross epithelial barrier and elicit harmful T cell-mediated immune response. Dendritic cells (DC) are supposed to play pivotal role shaping The direction response toward immunity or tolerance depends on stage maturation functional properties DC. DC become fully APC upon by various stimuli. We investigated effect peptic digest gliadin...