A5025306355- Gut microbiota and health
- Probiotics and Fermented Foods
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Ruminant Nutrition and Digestive Physiology
- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Studies on Chitinases and Chitosanases
- Cervical Cancer and HPV Research
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Bacterial Identification and Susceptibility Testing
- Molecular Biology Techniques and Applications
- Legume Nitrogen Fixing Symbiosis
- Phytase and its Applications
- Gynecological conditions and treatments
- Plant and fungal interactions
- Urological Disorders and Treatments
- Cancer Diagnosis and Treatment
- Multiple and Secondary Primary Cancers
- Protein Hydrolysis and Bioactive Peptides
- Bladder and Urothelial Cancer Treatments
- Microbial Community Ecology and Physiology
- Pelvic floor disorders treatments
- Ureteral procedures and complications
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2012-2023
Institute of Animal Physiology of the Slovak Academy of Sciences
1989-2012
Czech Academy of Sciences
1983-2010
Murdoch University
1998
Radboud University Nijmegen
1997
T. G. Masaryk Water Research Institute
1996
Research Institute of Inorganic Chemistry
1990
Institut für ökologische Wirtschaftsforschung
1986
Institute of Chemical Technology
1983
Jan Evangelista Purkyně University in Ústí nad Labem
1964
Abstract Ruminant livestock are important sources of human food and global greenhouse gas emissions. Feed degradation methane formation by ruminants rely on metabolic interactions between rumen microbes affect ruminant productivity. Rumen camelid foregut microbial community composition was determined in 742 samples from 32 animal species 35 countries, to estimate if this influenced diet, host species, or geography. Similar bacteria archaea dominated nearly all samples, while protozoal...
A 1000-cow study across four European countries was undertaken to understand what extent ruminant microbiomes can be controlled by the host animal and identify characteristics of rumen microbiome axis that determine productivity methane emissions. core microbiome, phylogenetically linked with a preserved hierarchical structure, identified. 39-member subset formed hubs in co-occurrence networks linking structure genetics phenotype (methane emissions, blood metabolites, milk production...
Summary Commensal bacteria have been shown to modulate the host mucosal immune system. Here, we report that oral treatment of BALB/c mice with components from commensal, Parabacteroides distasonis, significantly reduces severity intestinal inflammation in murine models acute and chronic colitis induced by dextran sulphate sodium (DSS). The membranous fraction P. distasonis (mPd) prevented DSS-induced increases several proinflammatory cytokines, increased mPd-specific serum antibodies...
Background Probiotic bacteria can be used for the prevention and treatment of human inflammatory diseases including bowel (IBD). However, nature active components exact mechanisms this beneficial effects have not been fully elucidated. Our aim was to investigate if lysate probiotic bacterium L. casei DN-114 001 (Lc) could decrease severity intestinal inflammation in a murine model IBD. Methodology/Principal Findings The preventive effect oral administration Lc significantly reduces acute...
Two novel Gram-negative, anaerobic, non-spore-forming, butyrate-producing bacterial species, strains Mz 5T and JK 615T, were isolated from the rumen fluid of cow sheep. Both curved rods that motile by means single polar or subpolar flagellum common in microbial ecosystem. Strain produced high xylanase, proteinase, pectin hydrolase DNase activities; 1,4-β-endoglucanase was also detected culture medium. The bacterium utilized a wide range carbohydrates. Glucose fermented to formate, butyrate,...
Several physical and chemical techniques were used to extract, identify the location of, proteolytic enzymes associated with mixed rumen bacteria. Most activity was removable by gentle methods such as shaking brief blending, without cell disruption, indicating that it coat capsular material. Proteases present also in envelope, corresponding inner membrane fraction of gram-negative bacteria, intracellularly detergent French press treatment. Temperature pH profiles obtained for enzymes, likely...
It is proposed that Clostridium proteoclasticum be reclassified as Butyrivibrio proteoclasticus comb. nov. on the basis of phylogenetic position, DNA G+C content and physiological traits. Phylogenetic analyses based 16S rRNA gene sequences from an extensive range taxa within clostridial subcluster XIVa grouped C. together with isolates genus Butyrivibrio, though this species was genetically distinct extant examined. The originally erroneously reported 28 mol%. However genome sequence type...
Three strains of a butyrate-producing bacterium were isolated from the rumen contents grazing sheep and cows. The anaerobic, with Gram-positive cell walls, straight-to-slightly-curved, rod-shaped, non-spore-forming single flagellate. C14 : 1, 0, C16 0 1 predominant fatty acids. cell-wall peptidoglycan type was A1γ. DNA G+C content varied 41.4 to 42.2 mol%. 16S rRNA gene sequence similarities between isolates Eubacterium rectale, Roseburia hominis intestinalis found be 96, 95 %, respectively....
Microbial sensing by Toll-like receptors (TLR) and its negative regulation have an important role in the pathogenesis of inflammation-related cancer. In this study, we investigated signaling gut microbiota development colitis-associated cancer mouse model.Colitis-associated was induced azoxymethane dextran sodium sulfate wild-type interleukin-1 receptor-associated kinase M (IRAK-M)-deficient mice with or without antibiotic (ATB) treatment. Local cytokine production analyzed multiplex assay...
Four strains of Butyrivibrio fibrisolvens, transformed with a gene encoding fluoroacetate dehalogenase, maintained combined population 10(6) to 10(7) cells ml-1 in the rumens test sheep. Five inoculated sheep showed markedly reduced toxicological symptoms after poisoning when behavioral, physiological, and histological effects were compared those five uninoculated control
Our previous study, based primarily on PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing, focused the isolation of four bifidobacterial groups from digestive tract three bumblebee species. In that we proposed these isolated potentially represented novel species family Bifidobacteriaceae. One four, Bifidobacterium bombi, has been described recently. Strains representing two other have classified as members genus basis positive results for fructose-6-phosphate...
Gram-positive-staining, anaerobic, non-spore-forming, lactate- and acetate-producing bacterial strains were isolated from the digestive tracts of different bumblebee species (Bombus lucorum, Bombus pascuorum lapidarius). All isolates produced fructose-6-phosphate phosphoketolase activity. A representative strain, BluCI/TPT, was characterized further. Cells strain BluCI/TPT showed occasional bifurcation irregular constrictions. The bacterium utilized a wide range carbohydrates. Glucose...
J. KOPEČNÝ, B. HODROVÁ AND C.S. STEWART. 1996. Chitinolytic bacteria were detected in faeces and digesta of wild domesticated herbivores. The presence chitinolytic two cows was verified following enrichment culture rumen fluid on colloidal chitin. In three other cows, direct counts chitin agar showed that the numbers these ranged from 5 × 104 to 2 108 ml−1. Most Clostridium-like spore producers. most typical strain, Clostridium sp. ChK5, characterized further. This bacterium degraded...