A5065432614- Gut microbiota and health
- Electron and X-Ray Spectroscopy Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Advanced Electron Microscopy Techniques and Applications
- Advanced Materials Characterization Techniques
- Inflammatory Bowel Disease
- Ion-surface interactions and analysis
- Advanced Surface Polishing Techniques
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Helicobacter pylori-related gastroenterology studies
- Semiconductor materials and devices
- Spondyloarthritis Studies and Treatments
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Catalytic Processes in Materials Science
- Surface and Thin Film Phenomena
- Immune Response and Inflammation
- Advanced Chemical Physics Studies
- Cytomegalovirus and herpesvirus research
- Dermatology and Skin Diseases
- nanoparticles nucleation surface interactions
- Microscopic Colitis
- Ocular Diseases and Behçet’s Syndrome
Czech Academy of Sciences, Institute of Microbiology
2014-2024
Czech Academy of Sciences
2013-2024
Charles University
2000-2021
Tescan (Czechia)
2011-2020
South Moravian Region
2020
University of Hradec Králové
2019
Semmelweis University
2007
University College London
2007
Ocní klinika
1991-1993
Jan Evangelista Purkyně University in Ústí nad Labem
1973
Mutations in the Nod2 gene are among strongest genetic risk factors pathogenesis of ileal Crohn's disease, but exact contributions to intestinal mucosal homeostasis not understood. Here we show that plays an essential role controlling commensal bacterial flora intestine. Analysis bacteria from terminal ilea Nod2-deficient mice showed they harbor increased load resident bacteria. Furthermore, had a diminished ability prevent colonization pathogenic In vitro, crypts isolated were unable kill...
Summary Commensal bacteria have been shown to modulate the host mucosal immune system. Here, we report that oral treatment of BALB/c mice with components from commensal, Parabacteroides distasonis, significantly reduces severity intestinal inflammation in murine models acute and chronic colitis induced by dextran sulphate sodium (DSS). The membranous fraction P. distasonis (mPd) prevented DSS-induced increases several proinflammatory cytokines, increased mPd-specific serum antibodies...
Abstract Background Mammals are essentially born germ-free but the epithelial surfaces promptly colonized by astounding numbers of bacteria soon after birth. The most extensive microbial community is harbored distal intestine. gut microbiota outnumber ~10 times total number our somatic and germ cells. host-microbiota relationship has evolved to become mutually beneficial. Studies in mice have shown that play a crucial role development immune system. principal aim present study was elucidate...
The aim was to analyze the influence of intestinal microbiota on development inflammation. We used model chronic inflammation that develops spontaneously in colon conventional severe combined immunodeficiency (SCID) mice restored with CD45 RB(high) subset CD4+T cells isolated from spleen normal BALB/c mice.A CD4+CD45RB(high) subpopulation T purified by magnetic separation (MACS) and transferred into immunodeficient SCID mice. Germ-free (GF) or monoassociated Enterococcus faecalis, SFB...
Microbial sensing by Toll-like receptors (TLR) and its negative regulation have an important role in the pathogenesis of inflammation-related cancer. In this study, we investigated signaling gut microbiota development colitis-associated cancer mouse model.Colitis-associated was induced azoxymethane dextran sodium sulfate wild-type interleukin-1 receptor-associated kinase M (IRAK-M)-deficient mice with or without antibiotic (ATB) treatment. Local cytokine production analyzed multiplex assay...
The microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies inflammatory response in murine model experimental autoimmune uveoretinitis (EAU). We induced EAU germ-free (GF) conventionally housed (CV) mice and CV treated with combination broad-spectrum antibiotics either from day induction one week prior to disease. severity inflammation was assessed by fundus biomicroscopy histology, including immunohistology. immunophenotyping T cells...
Diet is a strong modifier of microbiome and mucosal microenvironment in the gut. Recently, components western-type diets have been associated with metabolic immune diseases. Here, we studied how high-sugar diet (HSD) consumption influences gut barrier response under steady state conditions mouse model acute colitis. We found that HSD significantly increased permeability, spleen weight, neutrophil levels spleens healthy mice. Subsequent dextran sodium sulfate administration led to severe In...
The worldwide incidence of many immune-mediated and metabolic diseases, initially affecting only the wealthy Western countries, is increasing rapidly. Many these diseases are associated with compositional functional alterations gut microbiota, i.e., dysbiosis. most consistent markers dysbiosis a decrease in microbiota diversity an expansion Proteobacteria. role food preservatives as potential triggers has been long overlooked. Using human microbiota-associated mouse model, we demonstrate...
A healthy intestinal tract is characterized by controlled homeostasis due to the balanced interaction between commensal bacteria and host mucosal immune system. Human animal model studies have supported hypothesis that breakdown of this may underlie pathogenesis inflammatory bowel diseases. However, it not well understood how microflora stimulate system such activation regulated. Using a spontaneous, bacteria-dependent colitis in IL-10-deficient mice, we investigated role TLR their negative...
Summary One of the promising approaches in therapy ulcerative colitis is administration butyrate, an energy source for colonocytes, into lumen colon. This study investigates effect butyrate producing bacterium Clostridium tyrobutyricum on dextran sodium sulphate (DSS)-induced mice. Immunocompetent BALB/c and immunodeficient severe combined immunodeficiency (SCID) mice reared specific-pathogen-free (SPF) conditions were treated intrarectally with C. 1 week prior to induction DSS during oral...
To cite this article: Schwarzer M, Repa A, Daniel C, Schabussova I, Hrncir T, Pot B, Stepankova R, Hudcovic Pollak Tlaskalova-Hogenova H, Wiedermann U, Kozakova H. Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T-regulatory responses upon systemic sensitization. Allergy 2011; 66: 368–375. Abstract Background: The use recombinant lactic acid bacteria (LAB) as vehicles for mucosal delivery allergens is an attractive concept...
This study was aimed to evaluate the role of commensal Gram-negative bacterium Bacteroides ovatus in murine model chronic intestinal inflammation. The attempt induce colitis done ovatus-monoassociated, germ-free and conventional mice either immunocompetent (BALB/c) or with severe combined immunodeficiency (SCID), using 2.5 % dextran-sodium sulfate (DSS) drinking water (7 days DSS, 7 water, DSS). Conventional developed colitis. Some BALB/c majority SCID did not survive long-term treatment DSS...
Non-infectious uveitis is considered an autoimmune disease responsible for a significant burden of blindness in developed countries and recent studies have linked its pathogenesis to dysregulation the gut microbiota. We tested immunomodulatory properties two probiotics, Escherichia coli Nissle 1917 (EcN) E. O83:K24:H31 (EcO), model experimental (EAU). To determine importance bacterial viability treatment timing, mice were orally treated with live or autoclaved bacteria both preventive...
Focused ion beam (FIB) milling has been used to fabricate magnetic nanostructures (wires, squares, discs) from single layers (Co, permalloy) and spin-valve (permalloy/Cu/Co) multilayers (thicknesses 5–50 nm) prepared by sputtering deposition. Milled surfaces of metallic thin films typically exhibit residual roughness, which is also transferred onto the edges milled patterns. This can lead domain wall pinning influence magnetization behaviour nanostructures. We have investigated process FIB...
Recent research highlights the profound impact of gut microbiome on neuropsychiatric disorders, shedding light its potential role in shaping human behavior. In this study, we investigate appetite regulation using activity-based anorexia (ABA) mouse model nervosa (AN) - a severe eating disorder with significant health consequences. ABA was induced conventional, antibiotic-treated, and germ-free mice. Our results show clear influence expression four orexigenic (neuropeptide Y, agouti-related...