A5068179735- Ocular Diseases and Behçet’s Syndrome
- Cytomegalovirus and herpesvirus research
- Ocular Infections and Treatments
- Gut microbiota and health
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Food Security and Health in Diverse Populations
- Escherichia coli research studies
- Stress Responses and Cortisol
- Diet and metabolism studies
- Probiotics and Fermented Foods
- Mycobacterium research and diagnosis
- Infant Nutrition and Health
- Diabetes and associated disorders
- Adrenal Hormones and Disorders
- Tryptophan and brain disorders
- Liver Disease Diagnosis and Treatment
Czech Academy of Sciences, Institute of Microbiology
2019-2022
Czech Academy of Sciences
2018-2020
Charles University
2020
Czech Academy of Sciences, Institute of Physiology
2018
Diet is a major factor determining gut microbiota composition and perturbances in this complex ecosystem are associated with the inflammatory bowel disease (IBD). Here, we used gnotobiotic approach to analyze, how interaction between diet rich proteins influences sensitivity intestinal inflammation murine model of ulcerative colitis. We found that animal protein (aHPD) exacerbates acute dextran sulfate sodium (DSS)-induced colitis while plant (pHPD) does not. The deleterious effect aHPD was...
Diet is a strong modifier of microbiome and mucosal microenvironment in the gut. Recently, components western-type diets have been associated with metabolic immune diseases. Here, we studied how high-sugar diet (HSD) consumption influences gut barrier response under steady state conditions mouse model acute colitis. We found that HSD significantly increased permeability, spleen weight, neutrophil levels spleens healthy mice. Subsequent dextran sodium sulfate administration led to severe In...
Non-infectious uveitis is considered an autoimmune disease responsible for a significant burden of blindness in developed countries and recent studies have linked its pathogenesis to dysregulation the gut microbiota. We tested immunomodulatory properties two probiotics, Escherichia coli Nissle 1917 (EcN) E. O83:K24:H31 (EcO), model experimental (EAU). To determine importance bacterial viability treatment timing, mice were orally treated with live or autoclaved bacteria both preventive...
Mucosal surfaces are colonized by highly diverse commensal microbiota. Coating with secretory IgA (SIgA) promotes the survival of bacteria while it inhibits invasion pathogens. Bacterial coating could be mediated antigen-specific SIgA recognition, polyreactivity, and/or SIgA-associated glycans. In contrast to many in vitro studies, only a few reported effect glycans vivo. Here, we used germ-free antibody-free newborn piglets model compare protective SIgA, enzymatically removed N-glycans,...
Abstract Purpose The purpose of the project was to test influence probiotic bacteria Escherichia coli Nissle (EcN) on severity intraocular inflammation in experimental autoimmune uveitis (EAU). Methods EAU induced C57BL/6J mice by interphotoreceptor retinoid binding protein complete Freund´s adjuvant and pertussis toxin. grade (0–4) assessed clinically (21st 27th day) using otoscope connected camera histologically (28th hematoxylin eosin stained tissues. Probiotic EcN were administered...
Abstract Unlike the individual's genetic makeup, gut microbiota can be influenced, and thus used in disease therapy or even prevention. We have shown that absence reduction of modifies inflammatory response murine model experimental autoimmune uveoretinitis (EAU). Our further purpose project was to test influence probiotics on intensity intraocular inflammation EAU. Uveitis induced C57BL/6J mice by interphotoreceptor retinoid binding protein complete Freund's adjuvant with pertussis toxin....