A5036132696- Ocular Diseases and Behçet’s Syndrome
- Cytomegalovirus and herpesvirus research
- Retinal and Optic Conditions
- Systemic Lupus Erythematosus Research
- CNS Lymphoma Diagnosis and Treatment
- Poisoning and overdose treatments
- Lymphoma Diagnosis and Treatment
- Drug-Induced Ocular Toxicity
- Alcoholism and Thiamine Deficiency
- Mycobacterium research and diagnosis
- Ocular Oncology and Treatments
- Vasculitis and related conditions
- Ocular Infections and Treatments
- Bone and Joint Diseases
- Animal Ecology and Behavior Studies
- Sarcoidosis and Beryllium Toxicity Research
- Spondyloarthritis Studies and Treatments
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Traumatic Brain Injury and Neurovascular Disturbances
- Syphilis Diagnosis and Treatment
- Retinal Diseases and Treatments
- Retinoids in leukemia and cellular processes
- Inflammasome and immune disorders
- Glaucoma and retinal disorders
General University Hospital in Prague
2016-2025
Charles University
2015-2025
Palacký University Olomouc
2022
University of Aberdeen
2012
Aberdeen Royal Infirmary
2012
NHS Grampian
2012
Ocní klinika
2009
The microbiota is a crucial modulator of the immune system. Here, we evaluated how its absence or reduction modifies inflammatory response in murine model experimental autoimmune uveoretinitis (EAU). We induced EAU germ-free (GF) conventionally housed (CV) mice and CV treated with combination broad-spectrum antibiotics either from day induction one week prior to disease. severity inflammation was assessed by fundus biomicroscopy histology, including immunohistology. immunophenotyping T cells...
The aetiology of non‐infectious uveitis is usually multifactorial, involving both genetic and environmental risk factors. association human leucocyte antigens (HLA) with some types well recognized, such as HLA‐A29 Birdshot chorioretinitis, or HLA‐B27 acute anterior alone ankylosing spondylitis. monogenic associations uveitis, in Blau syndrome Haploinsufficiency A20, are extremely rare due to limited experience the disease difficult treat manage if inflammation sight‐threatening nature. paper...
Background Vitreoretinal lymphomas belong to the family of central nervous system (CNS) lymphomas. The optimal approach for treatment isolated primary vitreoretinal lymphoma is unclear because lack large comparative clinical series. Combination intravitreal and systemic chemotherapy recommended in many reports. aim our retrospective study was compare survival rate prognosis patients with without CNS involvement. Methods Twenty were observed between years 2004and2016, 10 lymphoma. To rates,...
To study the dynamics and clinical determinants of chronic retinal nerve fiber layer thickness (RNFL) loss after methanol-induced optic neuropathy.Prospective cohort study.All patients underwent complete ophthalmic evaluation including spectral-domain optical coherence tomography 3 times during 4 years observation: 4.9 (±0.6), 25.0 49.9 (±0.5) months discharge.Eighty-four eyes 42 survivors methanol poisoning, mean age (standard deviation) 45.7 (±4.4) years; 82 41 controls, 44.0 (±4.2)...
Non-infectious uveitis is considered an autoimmune disease responsible for a significant burden of blindness in developed countries and recent studies have linked its pathogenesis to dysregulation the gut microbiota. We tested immunomodulatory properties two probiotics, Escherichia coli Nissle 1917 (EcN) E. O83:K24:H31 (EcO), model experimental (EAU). To determine importance bacterial viability treatment timing, mice were orally treated with live or autoclaved bacteria both preventive...
Context: Methanol poisoning induces acute optic neuropathy with possible long-term visual damage.Objective: To study the dynamics and key determinants of pathway functional changes during 4 years after methanol poisoning.Methods: A total 42 patients confirmed (mean age 45.7 ± 4.4 years) were examined 4.9 0.6, 25.0 49.9 0.5 months discharge. The following tests performed: evoked potential (VEP), retinal nerve fiber layer (RNFL) measurement, brain magnetic resonance imaging (MRI), complete...
Purpose TO assess the diagnostic yield of vitreous fluid analysis and therapeutic effect pars plana vitrectomy in patients with suspected intraocular inflammation. Methods During 2004–2008, was performed 89 (101 eyes) out 1233 uveitis. Vitreous specimens were analyzed by cytologic histopathologic examination, microbiologic culture, polymerase chain reaction, antibody determination, flow cytometry. Vitrectomy 85 eyes for both purposes; remaining 16 underwent only vitrectomy. Preoperative...
Context: The influence of co-morbid conditions on the outcome acute methanol poisoning in mass outbreaks is not known.Objective: objective this to study impact burden co-morbidities, complications, and methanol-induced brain lesions hospital, follow-up, total mortality.Methods: All patients hospitalized with during a outbreak were followed prospective cohort until death or final follow-up after 6 years. age-adjusted Charlson co-morbidity index (ACCI) score was calculated for each patient. A...
Abstract Purpose To present a possible coincidence of cytomegalovirus retinitis and intraocular lymphoma in patient with systemic non-Hodgkin’s lymphoma. Case presentation A 47-year-old woman presented decreased visual acuity associated white retinal lesions both eyes. history pneumonia unknown aetiology closely preceded the deterioration vision. Five years previously was diagnosed follicular She treated chemotherapy regimen comprised cyclophosphamide, adriamycin, vincristin, prednisone...
Acute anterior uveitis (AAU) is a relatively common extra-musculoskeletal manifestation of axial spondyloarthritis (axSpA); however, data on the prevalence active sacroiliitis in patients with AAU are limited.102 and 39 healthy subjects (HS) underwent clinical assessment sacroiliac joint MRI. Patients absence were reassessed after two years. International Spondyloarthritis Society (ASAS) classification criteria for axSpA (regardless patient's age) expert opinion definitive diagnosis...
Purpose: The effect of acute methanol poisoning on the follow-up quality life survivors in mass outbreaks is not known. objective this to study impact visual and central nervous system (CNS) sequelae long-term health-related (QoL) survivors, its clinical determinants, dynamics.Materials methods: A total 54 patients with confirmed (mean age 46.7 ± 13.4 years, 9 females) were examined consequently three times within six-year prospective cohort compared 23 controls history chronic alcohol...
Autoimmune uveitis is a serious sightthreatening disease that in many cases fails to respond conventional immunosuppressive or biological therapy. Experimental models used research allow more detailed study of pathogenesis the autoimmune process and testing new therapeutic strategies. Recent results show infection can trigger diseases, some commensal microorganisms are essential causing activity. The aim this work was assess effect broadspectrum antibiotics - combination metronidazole...
Autoimmune uveitis is a leading cause of visual impairment in developed countries patients working age. Animal models experimental autoimmune (EAU) have been established to serve as useful template for novel therapeutic approaches.Experimental induced C57BL/6 mice by subcutaneous application interphotoreceptor retinoid binding protein complete Freund's adjuvant and pertussis toxin. Clinical histological grading used assess the inflammation intensity EAU.The protocol induction EAU hides...
In human, autoimmune uveitis is a leading cause of visual disability and ranks with diabetic retinopathy as major source blind registrations in developed countries. Since most cases non-infectious are considered to be or at least immune-mediated, the management such patients has rested on appropriate immunosuppression. Some patients, however, despite maximal immunotherapy, fail respond seriously intolerant drug therapies. its establishment 20 years ago, model experimental uveoretinitis...
Context Investigate whether 123I-ioflupane SPECT (DaT SPECT) has the potential as a marker of basal ganglia damage in acute methanol poisoning.Methods Prospective, single-centre, cohort study patients with confirmed poisoning was conducted. DaT performed twice semi-quantification using DaTQUANTTM and MRI-based volumetry calculated. Specific binding ratios (SBR) striatum, caudate nucleus, putamen were correlated laboratory parameters outcome, volumetric data, retinal nerve fibres layer (RNFL)...
Abstract Purpose The research in experimental models of autoimmune uveitis helps to find new therapeutical strategies. aim this study is compare the effect cyclophosphamide (CPA), mycophenolate mofetil (MMF) and golimumab on inflammation intensity mice model (EAU). Methods EAU was induced C57BL/6 by subcutaneous application IRBP (interphotoreceptor retinoid binding protein) complete Freund’s adjuvant pertussis toxin. CPA injected intraperitoneally a single dose 100 mg/kg, MMF daily 30 50...
Some malignant diseases may masquerade in the eye as uveitis. The purpose of talk is to give an overview possibilities diagnosis and treatment recognized syndromes. Among these, most frequent intraocular lymphoma, but also other haematological malignancies such leukaemia, thrombocytopenia, macroglobulinaemia or melanoma retinoblastoma, many others, are included. Diagnosis syndromes should be considered all patients with idiopathic corticosteroid‐resistant chronic Timely case improve...
Anti‐microbial drugs are in rare cases recognized as inducers of intraocular inflammation, including occlusive vasculitis. The purpose this talk to give overview clinical features and possibilities treatment these diseases.