A5027273015- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Xenotransplantation and immune response
- Virus-based gene therapy research
- Infant Nutrition and Health
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Asthma and respiratory diseases
- Invertebrate Immune Response Mechanisms
- Animal health and immunology
- Viral Infectious Diseases and Gene Expression in Insects
- IL-33, ST2, and ILC Pathways
- Clostridium difficile and Clostridium perfringens research
- Neurobiology and Insect Physiology Research
- Cell Adhesion Molecules Research
- Animal Genetics and Reproduction
- Escherichia coli research studies
- Food Allergy and Anaphylaxis Research
- Effects of Radiation Exposure
Czech Academy of Sciences, Institute of Microbiology
2015-2024
Czech Academy of Sciences
2011-2024
Engineering Academy of the Czech Republic
2024
University of Iowa
2000-2002
Charles University
1993-1996
The intestinal microbiota is known to influence postnatal growth. We previously found that a strain of Lactiplantibacillus plantarum (strain Lp WJL ) buffers the adverse effects chronic undernutrition on growth juvenile germ-free mice. Here, we report sustains malnourished conventional animals and supports both insulin-like factor–1 (IGF-1) insulin production activity. have identified cell walls isolated from , as well muramyl dipeptide mifamurtide, sufficient cues stimulate animal despite...
Abstract Porcine γδ T cells have two levels of TCRγδ expression. Whereas TCRγδmed are mostly CD2+CD8− and CD2+CD8+, TCRγδhi highly enriched for CD2−CD8−. This distribution is independent bacterial colonization it already established in the thymus prior to export periphery. Sorting cultivation experiments revealed that CD2−CD8− unable acquire CD2 CD8, whereas CD2+ subsets can gain or loose CD8. There also differential susceptibility proliferation between CD2− cells. Although almost do not...
Background: Mucosal mast cells (MC) are key players in IgE-mediated food allergy (FA). The evidence on the interaction between gut microbiota, MC and susceptibility to FA is contradictory. Objective: We tested hypothesis that commensal bacteria essential for migration their maturation impacting FA. Methods: development severity of symptoms was studied sensitized germ-free (GF), conventional (CV), mice mono-colonized with L. plantarum WCFS1 or co-housed CV mice. were phenotypically...
Abstract Cesarean-derived piglets were reared for 5 wk under germfree conditions or monoassociated with a benign Escherichia coli (G58-1) enterohemorrhagic strain (933D) derived from O157:H7, and immunized i.p. the T-dependent (TD) Ags fluorescein-labeled (FL) keyhole limpet hemocyanin trinitrophenylated (TNP) type 2 T-independent TNP-Ficoll FL-Ficoll. Only colonized showed an increase in serum IgG, IgA, IgM had Abs to FL, TNP, colonizing bacteria. While FL TNP appeared following...
Summary In this report, we describe 12 subpopulations of porcine γδ thymocytes based on their expression CD1, CD2, CD4, CD8‐isoforms and CD45RC. Our data suggest that can be divided into two major families: (a) one large family CD4 – could further subdivided according to the CD2/CD8αα phenotype (b) a small + bearing CD8αβ possessing certain unusual features in comparison with other thymocytes. Maturation within begins proliferation CD2 CD8 CD1 CD45RC common precursor. This developmental...
Abstract Based on studies of sheep, ileal Peyer’s patches (IPP) have been regarded as a type primary lymphoid tissue similar to the bursa Fabricius in chicken. Because bursectomy results B cell deficiency, we wondered whether resection IPP piglets would effect. Comparison IPP-resected, surgical shams and untreated germ-free piglets, all which were later colonized with defined commensal flora, demonstrated that did not alter level phenotype T cells tissues blood 10 wk after surgery....
ABSTRACT Artiodactyls possess GALT that appears in fetal life and is located at the extreme end of ileum. These IPP contain mostly B cells involute early postnatal life. Rabbits have a similarly lymphoid organ, called sacculus rotundus. Studies sheep rabbits led to concept lower hindgut represents primary tissue for necessary normal cell development, analogous bursa Fabricius. This review traces history observations theories existing concerning role GALT. We then recent data from piglets...
Abstract Successive colonization of the thymus by waves thymocyte progenitors has been described in chicken-quail chimeras and suggested from studies mice. In swine, we show that first CD3ε-bearing thymocytes appear on day 40 gestation (DG40). These early were CD3εhigh belonged to γδ T cell lineage. Mature αβ observed 15 days later (DG55), their occurrence was preceded appearance CD3εlow (DG45). Thereafter, transient changes subset composition (DG56-DG74), which can be explained a gap pro-T...
Abstract Since the actual combinatorial diversity in VH repertoire fetal piglets represents <1% of potential mice and humans, we wondered whether 1) complementarity-determining region 3 (CDR3) was also restricted; 2) CDR3 changed with age; 3) to what extent contributed preimmune VDJ repertoire. spectratyping sequence analyses 213 CDR3s recovered from >30 animals different ages showed that >95% resulted junctional diversity. Unlike sheep cattle, somatic hypermutation does...
Abstract B cell lymphogenesis in mammals occurs various tissues during development but it is generally accepted that operates by the same mechanism all tissues. We show swine, frequency of in-frame (IF) VDJ rearrangements differs among yolk sac, fetal liver, spleen, early thymus, bone marrow, and late thymus. All recovered analyzed on 20th day gestation (DG20) from sac were 100% IF. Those at DG30 liver >90% IF, this predominance cells with apparently a single IF rearrangement...
Abstract Porcine respiratory and reproductive syndrome virus (PRRSV) causes an extraordinary increase in the proportion of B cells resulting lymphoid hyperplasia, hypergammaglobulinemia, autoimmunity neonatal piglets. Spectratypic analysis from isolator piglets show a non-Gaussian pattern with preferential expansion clones bearing certain H chain third complementary region (HCDR3) lengths. However, only PRRSV-infected was nearly identical spectratype observed for all tissues. This result...
Abstract A course and a site of B cell development in swine are not firmly known. In this study, we show that lymphogenesis is located the bone marrow (BM). According to expression MHC class II (MHC-II), CD2, CD21, CD25, CD45RC, CD172a, workshop cluster (identification number) (SWC) 7, μHC, porcine BM cells were resolved into seven subsets representing sequential stages development. Profile rearrangement-specific products transcripts from sorted confirmed proposed developmental pathway. The...
Lymphocyte subsets isolated from germ-free piglets experimentally infected with swine influenza virus (SIV), porcine reproductive and respiratory syndrome (PRRSV) or circovirus type 2 (PCV2) were studied the profile of these among three infections was monitored. Germ-free used since their response could be directly correlated to viral infection. Because SIV are resolved even by colostrum-deprived neonates whereas PRRSV PCV2 not, as a benchmark for an effectively caused large increase in...
Porcine reproductive and respiratory syndrome virus (PRRSV) emerged about 30 years ago continues to cause major economic losses in the pork industry. The lack of effective modified live vaccines (MLV) allows pandemic continue. We have previously shown that wild strains PRRSV affect nascent T cell repertoire thymus, deplete clones recognizing viral epitopes essential for neutralization, while triggering a chronic, robust, but ineffective antibody response. Therefore, we hypothesized current...