A5035452886- RNA modifications and cancer
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Gestational Diabetes Research and Management
- Pneumocystis jirovecii pneumonia detection and treatment
- Tuberculosis Research and Epidemiology
- Animal Behavior and Welfare Studies
- Bacteriophages and microbial interactions
- Birth, Development, and Health
- Biochemical and Molecular Research
- Adenosine and Purinergic Signaling
- Cancer-related gene regulation
- Ammonia Synthesis and Nitrogen Reduction
- Ovarian cancer diagnosis and treatment
- Reproductive System and Pregnancy
- Diabetes and associated disorders
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Hybrid Renewable Energy Systems
- Health disparities and outcomes
- Genomics and Chromatin Dynamics
- Psoriasis: Treatment and Pathogenesis
- Urban Heat Island Mitigation
Stanford University
2021-2025
Bar-Ilan University
2014-2024
Universidade de São Paulo
2005
mRNA modification regulates pluripotency When stem cells progress from an embryonic pluripotent state toward a particular lineage, molecular switches dismantle the transcription factor network that keeps cell pluripotent. Geula et al. now show N6-methyladenosine (m6A), messenger RNA (mRNA) present on transcripts of factors, drives this transition. Methylation destabilized and limited their translation efficiency, which promoted timely decay naïve pluripotency. This m6A methylation was also...
Animals in the wild are able to subsist on pathogen-infected and poisonous food show immunity various diseases. These may be due their microbiota, yet we have a poor understanding of animal microbial diversity function. We used metagenomics analyze gut microbiota more than 180 species wild, covering diverse classes, feeding behaviors, geographies, traits. Using de novo metagenome assembly, constructed functionally annotated database 5000 genomes, comprising 1209 bacterial which 75% unknown....
Objective Gestational diabetes mellitus (GDM) is a condition in which women without are diagnosed with glucose intolerance during pregnancy, typically the second or third trimester. Early diagnosis, along better understanding of its pathophysiology first trimester may be effective reducing incidence and associated short-term long-term morbidities. Design We comprehensively profiled gut microbiome, metabolome, inflammatory cytokines, nutrition clinical records 394 before GDM diagnosis. then...
Abstract Background ADAR proteins are among the most extensively studied RNA binding proteins. They bind to their target and deaminate specific adenosines inosines. activity is essential, editing of a subset targets critical for viability. Recently, huge number novel were detected by analyzing next generation sequencing data. Most these sites located in lineage-specific genomic repeats, probably result overactivity enzymes, thus masking functional sites. In this study we aim identify set...
A-to-I RNA editing is an important post-transcriptional modification, known to be altered in tumors. It targets dozens of sites within miRNAs, some which impact miRNA biogenesis and function, as well many recognition sites. However, the full extent effect on regulation by miRNAs its behavior human cancers still unknown. Here we systematically characterized 10 593 samples across 32 cancer types normal controls. We find that majority previously reported show little no evidence for this...
Psoriasis is a chronic inflammatory skin disease, whose pathogenesis involves dysregulated interplay among immune cells, keratinocytes and environmental triggers, including microbiota. Bacterial fungal dysbiosis has been recently associated with several immune-mediated diseases psoriasis. In this comprehensive study, we investigated how different sampling sites methods reflect the uncovered microbiota composition. After establishing most suitable approach, further examined correlations...
Fragile X syndrome (FXS) is the most frequent inherited form of mental retardation. The cause for this X-linked disorder silencing fragile retardation 1 (fmr1) gene and absence protein (Fmrp). RNA-binding Fmrp represses translation, particularly in synapses. In Drosophila, interacts with adenosine deaminase acting on RNA (Adar) enzymes. Adar enzymes convert to inosine (A-to-I) modify sequence transcripts. Utilizing fmr1 zebrafish mutant (fmr1-/-), we studied Fmrp-dependent neuronal circuit...
Abstract The prevalent m 6 Am mRNA cap modification was recently identified as a valid target for removal by the human obesity gene FTO along with previously established A modification. However, deposition and dynamics of in regulating are unknown. Here, we investigate liver A/m methylomes mice fed on high fat Western-diet ob/ob mice. We find that levels elevated mice, genes which lost marking under overly downregulated, including two fatty-acid-binding proteins FABP2, FABP5. Furthermore,...
In recent years, the notion that ovarian carcinoma results from ovulation-induced inflammation of fallopian tube epithelial cells (FTECs) has gained evidence. However, mechanistic pathway for this process not been revealed yet. current study, we propose mutator protein activation-induced cytidine deaminase (AID) as a link between in FTECs and genotoxic damage leading to carcinogenesis. We show AID, previously shown be functional only B lymphocytes, is expressed under physiological...
The gradual accumulation of mutations by any a number mutational processes is major driving force divergence and evolution. Here, we investigate potentially novel process that based on the activity members AID/APOBEC family deaminases. This gene has been recently shown to introduce—in multiple types cancer—enzyme-induced clusters co-occurring somatic caused cytosine deamination. Going beyond mutations, hypothesized APOBEC3—following its rapid expansion in primates—can introduce unique...
ABSTRACT Enterococcus faecalis is a resident of the human gut, though upon translocation to blood or body tissues, it can be pathogenic. Here we discover and characterize two peptide-based quorum-sensing systems that transcriptionally modulate de novo purine biosynthesis in E. . Using comparative genomic analysis, find most enterococcal species do not encode this system; moraviensis , haemoperoxidus caccae three are closely related one systems, only encodes both systems. We show these...
Abstract The human gut microbiome is a diverse ecosystem that encompasses multiple domains of life and plays vital role in health. Due to technical limitations, most studies have focused on prokaryotes, overlooking bacteriophages other viruses. common method profile viruses assemble shotgun metagenomic reads - often from virus-enriched samples identify viral genomes de novo . While valuable, this resource-intensive reference-independent has limited sensitivity. To overcome these drawbacks,...
Abstract Gestational diabetes mellitus (GDM) is a condition in which non-diabetic women are diagnosed with glucose intolerance during pregnancy, typically the second trimester. GDM can lead to wide range of obstetrical and metabolic complications for both mother neonate 1 . Early identification risk, along better understanding its pathophysiology first trimester may be effective reducing incidence, as well associated short long term morbidities 2 Here, we comprehensively profiled gut...
Summary The increasing incidence of antibiotic-resistant Mycobacterium tuberculosis infections is a global health threat necessitating the development new antibiotics. Serine hydrolases (SHs) are promising class targets because their importance for synthesis mycobacterial cell envelope. We screened library small molecules containing serine-reactive electrophiles and identified narrow spectrum inhibitors M. tuberculous growth. Using these lead molecules, we performed competitive...
Abstract In recent years, the notion that serous ovarian carcinoma results from ovulation-induced inflammation of fallopian tube epithelial cells (FTECs) has gained evidence. However, mechanistic pathway leads to mutagenesis and genomic instability not been revealed yet. The mutator enzyme activation-induced cytidine deaminase (AID), which is known be involved in process immunoglobulin somatic hypermutations, also recently implicated inflammation-induced malignancies. current study, we...