A5076668330- RNA modifications and cancer
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Pulmonary Hypertension Research and Treatments
- Extracellular vesicles in disease
- RNA regulation and disease
- Cardiac tumors and thrombi
- Epigenetics and DNA Methylation
- Viral-associated cancers and disorders
- Genomics and Rare Diseases
- HVDC Systems and Fault Protection
- Genetics and Neurodevelopmental Disorders
- Cardiovascular Effects of Exercise
- Acute Myeloid Leukemia Research
- Renal Diseases and Glomerulopathies
- Hereditary Neurological Disorders
- Cancer-related Molecular Pathways
- Dermatological and Skeletal Disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- vaccines and immunoinformatics approaches
- Chronic Lymphocytic Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
Tel Aviv University
2013-2025
Sheba Medical Center
2016-2025
University of Chicago
2014-2018
Howard Hughes Medical Institute
2014
Edmond and Lily Safra Children's Hospital
2007
mRNA modification regulates pluripotency When stem cells progress from an embryonic pluripotent state toward a particular lineage, molecular switches dismantle the transcription factor network that keeps cell pluripotent. Geula et al. now show N6-methyladenosine (m6A), messenger RNA (mRNA) present on transcripts of factors, drives this transition. Methylation destabilized and limited their translation efficiency, which promoted timely decay naïve pluripotency. This m6A methylation was also...
Abstract N 6 ‐methyladenosine (m A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles metabolism. However, methods to precisely locate the m A remain limited. We present here a photo‐crosslinking‐assisted sequencing strategy (PA‐m A‐seq) more accurately define sites with modification. Using this strategy, we obtained high‐resolution map of human transcriptome. The resembles general distribution pattern observed previously, reveals new at base resolution. Our...
Eukaryotic transfer RNAs contain on average 14 modifications. Investigations of their biological functions require the determination modification sites and dynamic variations fraction. Base methylation represents a major class tRNA modification. Although many approaches have been used to identify base methylations, including sequencing, they are generally qualitative do not report information Dynamic mRNA modifications shown play important roles; yet, extent fractions has reported...
Methylation at the N6 position of adenosine (m6A) is a highly prevalent and reversible modification within eukaryotic mRNAs that has been linked to many stages RNA processing fate. Recent studies suggest m6A deposition proteins involved in pathway play diverse set roles either restricting or modulating lifecycles select viruses. Here, we report levels are significantly increased cells infected with oncogenic human DNA virus Kaposi's sarcoma-associated herpesvirus (KSHV). Transcriptome-wide...
Abstract Hepatocellular carcinoma (HCC) accounts for the majority of primary liver cancers and is characterized by high recurrence heterogeneity, yet its mechanism not well understood. Here we show that N 1 -methyladenosine methylation (m A) in tRNA remarkably elevated hepatocellular patient tumour tissues. Moreover, m A signals are increased cancer stem cells (CSCs) negatively correlated with HCC survival. TRMT6 TRMT61A, forming methyltransferase complex, highly expressed advanced tumours...
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. However, the mechanism is complex unclear. Here, we aimed to test our hypothesis that cardiac small extracellular vesicles (sEVs), particularly mesenchymal stromal cell-derived sEVs (cMSC-sEVs), contribute link between post-MI left ventricular dysfunction (LVD)
Abstract N6-methyladenosine (m6A) is the most abundant internal RNA modification in eukaryotic mRNAs and influences many aspects of processing. miCLIP (m6A individual-nucleotide resolution UV crosslinking immunoprecipitation) an antibody-based approach to map m6A sites with single-nucleotide resolution. However, due broad antibody reactivity, reliable identification from data remains challenging. Here, we present miCLIP2 combination machine learning significantly improve detection. The...
The transcription factor forkhead box P3 (FOXP3 in humans; Foxp3 mice) controls the development and function of regulatory T cells (Treg). In mice, CD4(+)CD25(-) do not express following TCR activation. Whether FOXP3 is a common activation-induced molecule human cells--hence Treg restricted--is currently controversial issue. As can significantly modulate cells, understanding mode (and regulation) expression vital. Here we show that conventional induction activation both restricted to...
Abstract N 6 ‐methyladenosine (m A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles metabolism. However, methods to precisely locate the m A remain limited. We present here a photo‐crosslinking‐assisted sequencing strategy (PA‐m A‐seq) more accurately define sites with modification. Using this strategy, we obtained high‐resolution map of human transcriptome. The resembles general distribution pattern observed previously, reveals new at base resolution. Our...