A5002713419- RNA Research and Splicing
- Connective tissue disorders research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Autism Spectrum Disorder Research
- Dermatological and Skeletal Disorders
- Nuclear Structure and Function
- Genetics and Neurodevelopmental Disorders
- Multiple Myeloma Research and Treatments
- Bat Biology and Ecology Studies
- Cryospheric studies and observations
- Lipid metabolism and biosynthesis
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Advancements in Materials Engineering
- Bone and Dental Protein Studies
- Tribology and Wear Analysis
- Transport Systems and Technology
- Advanced biosensing and bioanalysis techniques
- Hematopoietic Stem Cell Transplantation
- Pluripotent Stem Cells Research
- Effects of Environmental Stressors on Livestock
- Phytochemical compounds biological activities
International Institute of Molecular and Cell Biology
2020-2025
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2018-2023
University of Warsaw
2015-2023
FAM46C is one of the most frequently mutated genes in multiple myeloma. Here, using a combination vitro and vivo approaches, we demonstrate that encodes an active non-canonical poly(A) polymerase which enhances mRNA stability gene expression. Reintroduction into myeloma cell lines, but not its catalytically-inactive mutant, leads to broad polyadenylation stabilization mRNAs strongly enriched with those encoding endoplasmic reticulum-targeted proteins induces death. Moreover, silencing cells...
Among numerous enzymes involved in RNA decay, processive exoribonucleases are the most prominent group responsible for degradation of entire molecules. The role mammalian cytoplasmic 3'-5' exonuclease DIS3L at organismal level remained unknown. Herein, we established knock-in and knock-out mouse models to study functions mice. mice is indeed a subunit exosome complex, disruption which leads severe embryo degeneration death soon after implantation. These changes could not be prevented by...
Abstract TENT5C is a non-canonical cytoplasmic poly(A) polymerase highly expressed by activated B cells to suppress their proliferation. Here we measure the global distribution of tail lengths in responsive using Nanopore direct RNA-sequencing approach, showing that polyadenylates immunoglobulin mRNAs regulating half-life and consequently steady-state levels. upregulated differentiating plasma innate signaling. Compared with wild-type, Tent5c −/− mice produce fewer antibodies have diminished...
Abstract Gametogenesis is a process in which dysfunctions lead to infertility, growing health and social problem worldwide. In both spermatogenesis oogenesis, post-transcriptional gene expression regulation crucial. Essentially, all mRNAs possess non-templated poly(A) tails, whose composition dynamics (elongation, shortening, modifications) determine the fate of mRNA. Moreover, gametogenesis, especially represents unique instance complexity tails metabolism, with oocyte-specific waves...
Abstract Despite the widespread use of mRNA vaccines against COVID-19, little is known about metabolism therapeutic RNAs. Here we nanopore sequencing 1–3 to analyse individual molecules, focusing on their poly(A) tails. We show that Moderna mRNA-1273 vaccine 4 has a tail around 100 nucleotides, followed by an mΨCmΨAG sequence. In cell lines, undergoes rapid degradation initiated removal, CCR4–NOT-mediated deadenylation. However, in medically relevant preclinical models, particularly...
Abstract Cytoplasmic polyadenylation plays a vital role in gametogenesis; however, the participating enzymes and substrates mammals remain unclear. Using knockout knock-in mouse models, we describe essential of four TENT5 poly(A) polymerases fertility gametogenesis. TENT5B TENT5C play crucial yet redundant roles oogenesis, with double both genes leading to oocyte degeneration. Additionally, TENT5B-GFP females display gain-of-function infertility effect, multiple chromosomal aberrations...
Transport infrastructure is a critical threat to populations of animals; wildlife crossing structures are therefore commonly applied as measure sustain animal movements across roads and railways. Research on the efficiency needed provide scientific underpinnings conservation efforts. Year-round monitoring large underpass S69 expressway in western Polish Carpathians revealed that structure was mainly used by wild mammals (68.6 %), followed domestic animals (22.1 %) people (9.3 %). Wild (14...
Abstract Though mRNA vaccines against COVID-19 have revolutionized vaccinology and been administered in billions of doses, we know incredibly little about how are metabolized vivo . Here implemented enhanced nanopore Direct RNA sequencing (eDRS), to enable the analysis single Moderna’s mRNA-1273 molecules, giving information sequence poly(A) tails. We show that mRNA-1273, with all uridines replaced by N1-methylpseudouridine (mΨ), is terminated a long tail (~100 nucleotides) followed an...
Our understanding of animal adaptations to human pressure is limited by the focus on rare taxa, despite that common species are more significant in shaping structure, function and service provision ecosystems. Thus better their ecology behavioural adjustments central for drafting conservation actions. In this study, we used radio-telemetry 21 individuals (10 females, 11 males) provide data spatial ecology, habitat selection use roosts one commonest species, whiskered bat (Myotis mystacinus),...
Summary Role of dominant DIS3 mutations in multiple myeloma (MM) remains elusive. These decrease the exoribonucleolytic activity DIS3, a key nuclear RNA-degrading enzyme. Utilizing knock-in mice with clinical Dis3 G766R variant, we demonstrate an increased frequency aberrant chromosomal translocations B-cells, leading to plasmacytoma, early-stage MM model. DIS3-dependent display characteristics activation-induced deaminase (AID) activity. In samples mutations, driver genes also show...
Summary TENT5C is a non-canonical cytoplasmic poly(A) polymerase (ncPAP) upregulated in activated B cells and suppressing their proliferation. Herein we measured the global distribution of tail lengths responsive using modified Nanopore direct RNA-sequencing approach revealed that polyadenylates immunoglobulin mRNAs regulating steady-state levels. Consequently, deficient secrete less antibodies KO mice have diminished gamma globulin concentrations despite increased number CD138 high plasma...
Abstract Osteoblasts orchestrate bone formation by secreting dense, highly cross-linked type I collagen and other proteins involved in osteogenesis. Mutations Col1α1, Col1α2, or biogenesis factors lead to the human genetic disease, osteogenesis imperfecta (OI). Herein, we show that TENT5A gene, whose mutation is responsible for poorly characterized XVIII OI, encodes an active cytoplasmic poly(A) polymerase regulating induced during osteoblast differentiation KO osteoblasts are defective...
As microRNAs have emerged to be important regulators of molecular events occurring at the synapses, new questions about their regulatory effect on behavior araised. In present study, we show for first time that dysregulated specific targeting miR132 Mmp9 mRNA in mouse brain results increased level protein, which affects synaptic plasticity and has an memory formation. Our data points importance complex precise regulation by brain.
In this work, we aimed to determine the role of activin A during crucial events mouse embryogenesis and distinguish function protein zygotic origin one secreted by maternal reproductive tract. To end, recorded progression development phenotype Inhba knockout embryos compared them with heterozygotes wild-type using time-lapse imaging detection lineage-specific markers. We revealed that deficiency does not impair course rate blastocyst stage. form functional epiblast, as evidenced their...
Multiple myeloma (MM), one of the most frequent haematological malignancies, significantly increases risk bacterial infections due to treatment-related side effects, comorbidities and cancer-induced immune deficiencies. Recently, CD71
We applied radio-telemetry on 21 individuals (10 females and 11 males) to provide data the temporal activity patterns of whiskered bat (Myotis mystacinus) in Carpathian Mountains (southern Poland). tested whether sex, season weather conditions affect their July-September. On average, bats left day roosts 18.9 minutes after sunset returned 198.1 before sunrise. The mean length was 330 (SE±25) per night. Bats were active mainly (85%) during whole night; they rarely (15% nights) interrupted...
Abstract There is increasing evidence of mitochondrial dysfunction in autism spectrum disorders (ASD), but the causal relationships are unclear. In an ASD patient whose identical twin was unaffected, we identified a postzygotic mosaic mutation p.Q639* TRAP1 gene, which encodes chaperone HSP90 family. Additional screening 176 unrelated probands revealed variant male who had inherited it from healthy mother. Notably, newly generated knock-in Trap1 p.Q641* mice display ASD-related behavioral...
Abstract Though mRNA vaccines against COVID-19 have revolutionized vaccinology and been administered in billions of doses, we know incredibly little about how are metabolized vivo. Here implemented enhanced nanopore Direct RNA sequencing (eDRS), to enable the analysis single Moderna’s mRNA-1273 molecules, giving vivo information sequence poly(A) tails. We show that mRNA-1273, with all uridines replaced by N1-methylpseudouridine (mΨ), is terminated a long tail (~100 nucleotides) followed an...
Abstract DIS3 encodes an essential ribonucleic subunit of the nuclear exosome complex, responsible for degrading RNA in nucleus. Somatic mutations drive translocations B cells, leading to multiple myeloma (MM). Clinical data analysis reveals that 42% occur at three recurrent residues (D479, D488, and R780). These mutations, deactivating exonucleolytic activity, are never homozygous, often appearing as minor subclones advanced MM. Surprisingly, mutant alleles undergo loss-of-heterozygosity,...
Summary Cytoplasmic polyadenylation plays a vital role in gametogenesis, however, the participating enzymes and substrates mammals remain unclear. Using knockout knock-in mouse models, we describe essential of 4 TENT5 poly(A) polymerases mice fertility gametogenesis. TENT5B TENT5C play crucial, but redundant roles oogenesis, with double both genes leading to oocyte degeneration. Additionally, TENT5B-GFP females display gain-of-function infertility effect multiple chromosomal aberrations...